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Showing: 6351 - 6400 of 13576 results

  6. pCOA-Leu-FvPPT1-fsr1

    Plasmid
    #179868
    Purpose
    Constitutive expression vector for Saccharomyces cerevisiae comprising the polyketide synthase fsr1 from Fusarium vanettenii and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1
    Depositor
    Jens Laurids Sørensen
    Article
    Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)
    Inserts
    PPT1
    fsr1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Codon optimized for expression in Saccharomyces c…
    Promoter
    Yarrowia lipolytica EXP1 and Yarrowia lipolytica …
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    179868-plasmid-map-sequence-id-352936

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  10. LeGO-iBSD-5LO-d4

    Plasmid
    #182743
    Purpose
    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform delta 4) in mamallian cells with blasticidin S resistance (bsd).
    Depositor
    Dieter Steinhilber
    Article
    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d
    Insert
    5-Lipoxygenase isoform delta 4 (ALOX5 Human)
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon optimized for expression in homo sapiens
    Promoter
    SFFV
    Available since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182743-plasmid-map-sequence-id-367810

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  11. LeGO-iBSD-5LO-d13

    Plasmid
    #182745
    Purpose
    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform delta 13) in mamallian cells with blasticidin S resistance (bsd).
    Depositor
    Dieter Steinhilber
    Article
    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d
    Insert
    5-Lipoxygenase isoform delta 13 (ALOX5 Human)
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon optimized for expression in homo sapiens
    Promoter
    Available since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182745-plasmid-map-sequence-id-367797

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  12. pGFP-HsSyx3

    Plasmid
    #179289
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + GFP + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Use
    Tags
    His6 + GFP + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    residues 393-792 from accession number NP_0010361…
    Promoter
    T7
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    179289-plasmid-map-sequence-id-354922

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  13. pB80-KIF1A(1-365)-GCN4-GFP-SSPB(micro)

    Plasmid
    #174632
    Purpose
    Highly processive KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-GCN4-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)
    Use
    Tags
    GFP-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; EGFP: Met1Del; SSPB:…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174632-plasmid-map-sequence-id-346976

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  14. pDONR221-SLC35A4_STOP

    Plasmid
    #161289
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    Giulio Superti-Furga, RESOLUTE Consortium
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC35A4 (SLC35A4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161289-plasmid-map-sequence-id-337938

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  15. pDONR221-SLC26A9_STOP

    Plasmid
    #161306
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    Giulio Superti-Furga, RESOLUTE Consortium
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC26A9 (SLC26A9 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161306-plasmid-map-sequence-id-312546

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  16. pDONR221-SLC35A5_STOP

    Plasmid
    #161277
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    Giulio Superti-Furga, RESOLUTE Consortium
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC35A5 (SLC35A5 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161277-plasmid-map-sequence-id-312488

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  17. pDONR221-SLC22A31_STOP

    Plasmid
    #161202
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    Giulio Superti-Furga, RESOLUTE Consortium
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC22A31 (SLC22A31 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161202-plasmid-map-sequence-id-312338

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  18. pDONR221-SLC25A18_STOP

    Plasmid
    #161169
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    Giulio Superti-Furga, RESOLUTE Consortium
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC25A18 (SLC25A18 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161169-plasmid-map-sequence-id-312272

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  19. pDONR221-SLC24A3_STOP

    Plasmid
    #161144
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    Giulio Superti-Furga, RESOLUTE Consortium
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC24A3 (SLC24A3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161144-plasmid-map-sequence-id-312222

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  20. pUC18 Apln5-HA-FlpO-pA-LoxP-PGK-Neo-pA-LoxP-Apln3 (SO89)

    Plasmid
    #159222
    Purpose
    Can be used to target the mouse Apln locus in mouse eggs or ES cells, in order to drive the mosaic expression of the protein HA-NLS-FlpO
    Depositor
    Rui Benedito
    Article
    Luo et al Nature. 2021 Jan;589(7842):437-441. doi:
    Insert
    Apln 5'-FlpO-WPRE-Sv40pA-LoxP-PGK-Neo-pA-LoxP-Apln
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Apln
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    159222-plasmid-map-sequence-id-315768

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  21. pMX-mPGK-CD3-P2A-CD90.2

    Plasmid
    #163337
    Purpose
    Retroviral vector to overexpress the murine CD3 (TCR) components CD3 gamma, CD delta, CD epsilon and TCR zeta and surface marker CD90.2, separated by T2; F2A; E2A; P2A respectively, under control of the mPGK promoter
    Depositor
    Teunis Geijtenbeek, Louis Ates
    Article
    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.
    Insert
    CD3
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mPGK
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    163337-plasmid-map-sequence-id-322033

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  22. pMX-hFTH1-CD3-P2A-CD90.2

    Plasmid
    #163335
    Purpose
    Retroviral vector to overexpress the murine CD3 (TCR) components CD3 gamma, CD delta, CD epsilon and TCR zeta and surface marker CD90.2, separated by T2; F2A; E2A; P2A respectively, under control of the hFTH1 promoter
    Depositor
    Teunis Geijtenbeek, Louis Ates
    Article
    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.
    Insert
    CD3
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hFTH1
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    163335-plasmid-map-sequence-id-322031

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  24. pcDNA3-sACE2v5

    Plasmid
    #145152
    Purpose
    Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 5
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145152-plasmid-map-sequence-id-292590

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  25. pcDNA3-sACE2-T92Q

    Plasmid
    #145156
    Purpose
    Mammalian expression plasmid for soluble ACE2 (protease domain) T92Q glycosylation mutant
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145156-plasmid-map-sequence-id-292616

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  26. 289aa ELL2

    Plasmid
    #127266
    Purpose
    For in vitro translation of shorter human ELL2 from Met1. Also contains Met133I, M1381I, M186I mutations.
    Depositor
    Christine Milcarek
    Article
    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi
    Insert
    NH2-ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)
    Use
    In vitro translation
    Tags
    Expression
    Mutation
    Three Mets (133, 138, 186) to Ileu. Contains 289…
    Promoter
    T7
    Available since
    July 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    127266-plasmid-map-sequence-id-250216

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  28. HA tagged Sph ELL2 Met133, 138, 186 to Ileu

    Plasmid
    #127271
    Purpose
    For in vitro translation of human ELL2 with Met133, 138, 186 to Ileu with HA tag after 8th Met
    Depositor
    Christine Milcarek
    Article
    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi
    Insert
    ELL2 Met133, 138, 186 to Ileu (ELL2 Human)
    Use
    In vitro translation
    Tags
    Expression
    Mutation
    Met133, 138, 186 to Ileu with HA tag after 8th Met
    Promoter
    T7
    Available since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    127271-plasmid-map-sequence-id-250013

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  29. HA tagged mid ELL2 M133, 138, 186ILeu

    Plasmid
    #127276
    Purpose
    Expresses human ELL2 with M133, 138, 186ILeu to prevent internal Met initiation peptides and contains middle HA tag that doesn't disrupt function
    Depositor
    Christine Milcarek
    Article
    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi
    Insert
    ELL2 (ELL2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    M133, 138, 186 to Ileu, HA tag at XbaI after Met …
    Promoter
    pEF1a
    Available since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    127276-plasmid-map-sequence-id-249998

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  30. pUC19-Q03JI6

    Plasmid
    #103123
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    Q03JI6 (Cas9 coding gene from Campylobacter jejuni)
    Use
    CRISPR; Cloning vector
    Tags
    SV40NLS
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103123-plasmid-map-sequence-id-201885

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  32. p426_Cas9_gRNA-R-ARS805a

    Plasmid
    #87408
    Purpose
    p426_Cas9_gRNA-ARS805a without ribozyme All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS805a sequence TTATTTGAATGATATTTAGT in yeast chromosome 8.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS805a
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87408-plasmid-map-sequence-id-193450

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Showing: 6351 - 6400 of 13576 results