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pGlnA-Ypet

Plasmid

#98278

Purpose

mammalian expression of GlnA-Ypet

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

GlnA

Tags

Ypet

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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5HRE/GFP

Plasmid

#46926

Purpose

hypoxia-responsive enhanced green fluorescent protein (EGFP)-based system

Depositor

Martin Brown, Thomas Foster

Article

Vordermark et al Neoplasia. 2001 Nov-Dec;3(6):527-34.

Insert

5X HRE of VEGF (VEGFA Human)

Tags

d2EGFP

Expression

Mammalian

Mutation

five copies of a 35-bp fragment from the hypoxia-…

Promoter

EF-1α promoter

Available Since

Aug. 30, 2013

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab7A

Plasmid

#28047

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rab7A (RAB7A Human)

Tags

2xHA and EGFP

Expression

Mammalian

Available Since

May 27, 2011

Availability

Academic Institutions and Nonprofits only

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pMUH_unc84_tdTFlag

Plasmid

#46024

Depositor

Sean Eddy

Article

Henry et al Nucleic Acids Res. 2012 Oct;40(19):9691-

Insert

UNC-84 (unc-84 )

Use

10xuas-phi31-expression vector

Tags

tdTomato

Mutation

Two amino acid mutations (F279L and L500P) compar…

Promoter

10XUAS

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pOG231

Plasmid

#17736

Depositor

Geoff Wahl

Article

O'Gorman et al Proc Natl Acad Sci U S A. 1997 Dec 23;94

Insert

NLS-Cre recombinase

Use

Cre/Lox

Expression

Bacterial

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
Lentiviral-TOP-dGFP-reporter

Plasmid

#14715

Purpose

Beta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).

Depositor

Tannishtha Reya

Article

Reya et al Nature. 2003 May 22. 423(6938):409-14.

Insert

LEF-1/TCF-responsive promoter (Ctnnb1 )

Use

Lentiviral

Tags

d2 eGFP

Expression

Mammalian

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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LM670

Plasmid

#191552

Purpose

LM670_MSG-AGGA-promoter-ccdb-TTCC-GShis

Depositor

David Baker

Article

Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

Insert

ccdb

Tags

HIS-tag

Expression

Bacterial

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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TNFa-GFP

Plasmid

#163660

Purpose

Expresses GFP tagged TNFa in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

tumor necrosis factor (TNF Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.nucGAP

Plasmid

#78736

Purpose

Expression of Ca2+ sensor GAP in the nucleus

Depositor

Teresa Alonso

Article

Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Insert

nucGAP

Tags

nucleoplasmin (Xenopus)

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
eIF2a 2

Plasmid

#21808

Depositor

David Ron

Insert

eIF2a (EIF2S1 Human)

Expression

Mammalian

Mutation

S52A

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pJYDN2

Plasmid

#162454

Purpose

Yeast display plasmid intended for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA) and expression of N-terminal fusions with Aga2p yeast agglutinin.

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Type

Empty backbone

Use

Shuttle vector bacteria/yeast

Tags

DnbALFA nanobody and Ha and myc

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLY095-EFS-ERBB2 HER2-Blast-WPRE

Plasmid

#192196

Purpose

Lenti-HER2-OE-Blast

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Lenti-HER2-OE-Blast (ERBB2 Synthetic)

Use

Lentiviral; Mammalian expression

Mutation

NA

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-LMP2A-IRES-GFP

Plasmid

#220352

Purpose

Expresses EBV latent gene LMP2A in mammalian cells

Depositor

Klaus Rajewsky

Article

Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

Insert

LMP2A (LMP-2A Epstein-Barr Virus (EBV) strain B95-8)

Use

Retroviral

Expression

Mammalian

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO SV40 small + Large T (w612-1)

Plasmid

#22298

Depositor

Eric Campeau

Insert

SV40 small + Large T antigen

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 15, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-FLAG-mRuby2

Plasmid

#175294

Purpose

Track mature and nascent FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with mRuby2

Tags

mRuby2

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDUAL CLDN (GFP)

Plasmid

#86981

Purpose

Lentiviral expression construct encoding Claudin-1 and GFP from separate promoters

Depositor

Joe Grove

Insert

CLDN1 (CLDN1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV/hPGK

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-ATF6

Plasmid

#11975

Purpose

For mammalian expression of human ATF6 with a 3x FLAG tag

Depositor

Ron Prywes

Article

Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

Insert

ATF6 (ATF6 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only

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pTK299

Plasmid

#59568

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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mEmerald-Occludin-C-14

Plasmid

#54211

Purpose

Localization: Tight Junctions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Occludin (OCLN Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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Human ICAM-1

Plasmid

#8632

Depositor

Timothy Springer

Article

Staunton et al Cell 1988 Mar 25;52(6):925-33.

Insert

ICAM-1 (ICAM1 Human)

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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