We narrowed to 13,298 results for: EGFP

1. pHIV-EGFP.Flag-EZH2-mt2*

   Plasmid

   #220246

   Purpose

   Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   EZH2 mt 2* (EZH2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   PRKKKR494-499NAAIRS

   Promoter

   EF-1α

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7-EGFP-C1-HsDCP2-E148Q_U

   Plasmid

   #147650

   Purpose

   Mammalian Expression of HsDCP2-E148Q

   Depositor

   Elisa Izaurralde

   Article

   Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

   Insert

   HsDCP2-E148Q (DCP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   two silent mutations compared to the sequence giv…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET22b-mEB2-eGFP-6xHis

   Plasmid

   #130982

   Purpose

   Bacterial expression of mouse EB2-GFP-6xHis for protein purification.

   Depositor

   Anne Straube

   Article

   Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

   Insert

   EB2-GFP-6xHis (Mapre2 Mouse)
   

   Use

   Tags

   eGFP-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP C3-Lats1-PY1+2 mutant

   Plasmid

   #19054

   Depositor

   Marius Sudol

   Article

   Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

   Insert

   Large tumor suppressor kinase 1, N mutant (LATS1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   PY1 mutant, (Tyrosine 376 changed to Alanine) and…

   Promoter

   Available since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pEGFP-C1-Cep123 (CCDC123/Cep89)

   Plasmid

   #67787

   Purpose

   This plasmid encodes EGFP fused to the N-terminus of isoform 1 of Cep123 (CCDC123/Cep89)

   Depositor

   Michel Bornens

   Article

   Sillibourne et al Biol Open. 2013 Apr 9;2(6):535-45. doi:

   Insert

   Cep123 (CEP89 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEZYeGFP-SARS-CoV-2E GGG

   Plasmid

   #224582

   Purpose

   To express a GFP tagged version of the Envelope protein of SARS-CoV-2 with a mutated PBM in mammalian cells

   Depositor

   Isabel Mérida

   Article

   Avila-Flores et al Int J Mol Sci. 2023 Aug 14;24(16):12793.

   Insert

   SARS-CoV-2E GGG (E SARS-CoV-2)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   PBM (LLV) mutated to GGG

   Promoter

   CMV

   Available since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMAM-RecG-ST2TaV-EGFP

   Plasmid

   #31275

   Depositor

   Andrew Pierce

   Article

   Killen et al BMC Mol Biol. 2012 Oct 30;13:33. doi: 10

   Insert

   RecG-ST2-TaV-EGFP
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAM-AAV-CAG-EGFP

   Plasmid

   #153186

   Purpose

   CAG promoter-mediates gene expression in retinal neurons with fluorescent reporter

   Depositor

   Yang Hu

   Article

   Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-NMHC-IIA-3xA

   Plasmid

   #101041

   Purpose

   Expresses GFP-MYH9 construct (human myosin IIA) carrying S1943A mutation and S1916A, S1915A mutation, driven by CMV promoter. Thus inhibits CKII and PKC phosphorylation of the tail.

   Depositor

   Tom Egelhoff

   Article

   Rai et al J Biol Chem. 2017 Feb 24;292(8):3099-311

   Insert

   Non-muscle myosin IIA heavy chain (MYH9 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   S1915A, S1916A, S1943A

   Promoter

   CMV

   Available since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. LifeAct-EGFP-APEX2 in pCSDEST2

    Plasmid

    #170523

    Purpose

    Vector for expression of LifeAct APEX2 in mammalian cells. Parton lab clone FTE

    Depositor

    Rob Parton

    Article

    Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

    Insert

    Cavin4 (Cavin4 Mouse)
    

    Use

    Tags

    APEX2-P2A-mKate2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDGB3_alpha1_35S-eGFP-T35S (GB4279)

    Plasmid

    #225434

    Purpose

    Transcriptional unit for constitutive expression of the enhanced GFP.

    Depositor

    Diego Orzaez

    Insert

    35S:eGFP:T35S
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AICSDP-144:CTCF-mEGFP

    Plasmid

    #168799

    Purpose

    Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human CTCF

    Depositor

    Allen Institute for Cell Science

    Insert

    CTCF (CTCF Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    June 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pGL4.10-VEGFprom(-1000 to -500)

    Plasmid

    #66129

    Purpose

    Luciferase-based reporter for VEGF promoter region (-1000 to -500 bp)

    Depositor

    David Mu

    Article

    Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

    Insert

    Vascular Endothelial Growth Factor -A promoter region (VEGFA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    VEGF -500 to -1

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT7-EGFP-C1-HsXRN1_1-1173_K

    Plasmid

    #146785

    Purpose

    Mammalian Expression of HsXRN1_1-1173

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

    Insert

    HsXRN1_1-1173 (XRN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    Nov. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-eGFP-SynaptoZip

    Plasmid

    #122525

    Purpose

    AAV expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by human Synapsin promoter

    Depositor

    Antonio Malgaroli

    Article

    Ferro et al Nat Commun. 2017 Oct 31;8(1):1229. doi:

    Insert

    eGFP-SynaptoZip (Vamp2 Rat)
    

    Use

    AAV

    Tags

    Myc and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEMHE-mEGFP-mTrim21ΔRING-Box

    Plasmid

    #105520

    Purpose

    T7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted RING-box mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    TRIM21 (Trim21 Mouse)
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Expression

    Mutation

    deletion of RING domain (aa124-462)

    Promoter

    T7

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS-CIBN-EGFP-KRasCT

    Plasmid

    #178527

    Purpose

    Expresses CIBN-EGFP-KRasCT in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    CIBN-EGFP-KRasCT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. S9_attL1-EGFP-C-term-attL2

    Plasmid

    #128473

    Purpose

    EGFP entry clone

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    EGFP entry clone
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR-L3-Csy4-Cas9n-EGFP-L2

    Plasmid

    #141046

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Csy4-2A-Cas9n-2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTYF-mCMV/GfaABC1D-EGFP

    Plasmid

    #19976

    Depositor

    Sergey Kasparov

    Article

    Liu et al BMC Biotechnol. 2008 May 16;8:49. doi: 1

    Insert

    mCMVGfaABC1D-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

21. pcDNA5-eGFP-PC1-3HA

    Plasmid

    #108406

    Purpose

    Can be used to generate stable Flp-In cells with tetracycline-inducible human PC1 expression.

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Human)
    

    Use

    Tags

    3HA and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPRISM-TagRFP-gcry-eGFP

    Plasmid

    #117767

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    TagRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pT7-EGFP-C1-HsXRN1_1174-1706_K

    Plasmid

    #146788

    Purpose

    Mammalian Expression of HsXRN1_1174-1706

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

    Insert

    HsXRN1_1174-1706 (XRN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBS-f1(-)-GAPDH-IRES-EGFP

    Plasmid

    #115918

    Purpose

    donor to insert IRES-EGFP at the GAPDH locus in human cells

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    IRES-EGFP
    

    Use

    Donor template

    Tags

    dTomato

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAG-SRGAP1FBAR(516a.a.)-EGFP

    Plasmid

    #168505

    Purpose

    Expression of F-BAR domain (aa1-516) of SRGAP1 with a C-terminal EGFP fusion.

    Depositor

    Franck Polleux

    Article

    Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

    Insert

    SRGAP1-FBAR (SRGAP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 1-516

    Promoter

    CAG

    Available since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pME-EGFP-P2A-APEX2-NS

    Plasmid

    #108895

    Purpose

    Multisite gateway vector for 5' tagging with APEX2 (includes cytoplasmic GFP reporter)

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    EGFP-P2A-APEX2
    

    Use

    Multisite gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEGFP-C1 beta-catenin Y654E

    Plasmid

    #16073

    Depositor

    Erin Schuman

    Article

    Murase et al Neuron. 2002 Jul 3. 35(1):91-105.

    Insert

    beta-catenin (CTNNB1 Chicken)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Y654E, *(see below)

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Actin-53a-ires-EGFP

    Plasmid

    #25460

    Depositor

    Jerry Crabtree

    Article

    Wu et al Neuron. 2007 Oct 4. 56(1):94-108.

    Insert

    BAF53a (Actl6a Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSICO-CPSF6-358-eGFP

    Plasmid

    #110693

    Purpose

    Expresses a truncated form of CPSF6 (CPSF6-358 originally described in Lee et al. Cell Host Microbe 2010) fused with eGFP

    Depositor

    Zandrea Ambrose

    Article

    Ning et al J Virol. 2018 Apr 11. pii: JVI.00368-18.

    Insert

    CPSF6-eGFP (CPSF6 )
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Truncation of CPSF6 isoform 2 at amino acid 358

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSG81-pLas-tetO-deGFPssrA

    Plasmid

    #102454

    Purpose

    Expresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pLas-tetO combinatorial promoter.

    Depositor

    Richard Murray

    Article

    Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

    Insert

    deGFP
    

    Use

    Tags

    ssrA

    Expression

    Bacterial

    Mutation

    Promoter

    pLas-tetO

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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