2,226 results

1. Shank3-HA/pRK5

   Plasmid

   #197335

   Purpose

   Plasmid expressing an antigen targeting Shank3

   Depositor

   Morgan Sheng

   Insert

   Shank3 (Shank3 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5-FLAG-S6K1

   Plasmid

   #100509

   Purpose

   Expresses FLAG tagged S6K1 in mammalian cells

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   S6K1 (Rps6kb1 Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBiFC-bJunVN155(I152L)

   Plasmid

   #27098

   Depositor

   Chang-Deng Hu

   Article

   Kodama et al Biotechniques. 2010 Nov . 49(5):793-805.

   Insert

   bJunVN155(I152L) (Jun Rat, and Aequorea Victoria)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   I152L in Venus(1-154)

   Promoter

   Available since

   Jan. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. FU- dio PSD95-mCherry- W

   Plasmid

   #73919

   Purpose

   Expresses PSD-95 tagged with mCherry. Expression is Cre dependent. Plasmid can also be used to make lentivirus.

   Depositor

   Elly Nedivi

   Article

   Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

   Insert

   PSD-95-mCherry
   

   Use

   Lentiviral

   Tags

   mCherry and myc

   Expression

   Mammalian

   Mutation

   Promoter

   Ubi-C

   Available since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-XI-Neo-GST- AMPK- Kinase Dead (K to R)

   Plasmid

   #139844

   Purpose

   Lentiviral expression of GST-tagged rat AMPK- Kinase Dead (K to R)

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   AMPK-Kinase-dead (Prkaa2 Rat)
   

   Use

   Lentiviral

   Tags

   GST

   Expression

   Mutation

   K47R

   Promoter

   Available since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. G protein alpha-s-GFP

   Plasmid

   #66083

   Purpose

   G protein alpha-s internally tagged with EGFP and EE epitope

   Depositor

   Catherine Berlot

   Article

   Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

   Insert

   G-alpha-s-EE-EGFP (Gnas Rat)
   

   Use

   Tags

   EGFP flanked by SGGGS on each side was inserted i…

   Expression

   Mammalian

   Mutation

   BamHI sites in the alpha-s cDNA were removed and …

   Promoter

   CMV

   Available since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-fDIO-GCaMP6s

   Plasmid

   #105714

   Purpose

   Can be used to express GCaMP6s in the presence of Flp recombinase. Can be used to create adeno-associated virus to express Flp-dependent GCaMP6s.

   Depositor

   Rylan Larsen

   Has Service

   AAV8

   Insert

   GCaMP6s
   

   Use

   AAV; Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a, Human elongation factor-1 alpha

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-mEGFP-CaMKIIa (T286A)

   Plasmid

   #127390

   Purpose

   Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286A)

   Depositor

   Ryohei Yasuda

   Article

   Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

   Insert

   calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   changed Threonine 286 to Alanine

   Promoter

   pCAG

   Available since

   Jan. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHFS:SEP-rGluA2 TKIT donor

   Plasmid

   #169440

   Purpose

   Donor DNA for TKIT knockin of SEP to GluA2 in rat

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   Super ecliptic pHluorin (SEP)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-CMV-jGCaMP7c variant 1513

    Plasmid

    #105320

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Insert

    jGCaMP7c variant 1513
    

    Use

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. miniCGBE1-SpRY (pBM1571)

    Plasmid

    #170105

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1 and SpRY-nCas9 (D10A/A61R/L1111R…

    Promoter

    CMV

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.CAG.iGluSnFR3.v857.PDGFR

    Plasmid

    #178333

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV CAG iGluSnFR3 v857.PDGFR
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and PDGFR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-NR1a

    Plasmid

    #17926

    Depositor

    Stefano Vicini

    Article

    Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

    Insert

    glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A (Grinl1a Rat)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. mApple-Lysosomes-20

    Plasmid

    #54921

    Purpose

    Localization: Lysosome Membrane, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    LAMP1
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pRK7-HA-S6K1-KR

    Plasmid

    #8985

    Depositor

    John Blenis

    Article

    Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

    Insert

    S6K1 (Rps6kb1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K100R, kinase-inactive

    Promoter

    Available since

    Jan. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pClneoMyc rat S-SCAM alpha

    Plasmid

    #40213

    Depositor

    Yutaka Hata, Yoshimi Takai

    Article

    Hirao et al J Biol Chem. 1998 Aug 14;273(33):21105-1

    Insert

    S-SCAM (Magi2 Rat)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pBactin-AcGFP-128-425-Akap9

    Plasmid

    #196871

    Purpose

    Expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to AcGFP. Used to displace endogenous Akap9 from Golgi

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    AcGFP-Akap9 (128-425) (Akap9 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGP-AAV-syn-jGCaMP7c variant 1513-WPRE

    Plasmid

    #105321

    Purpose

    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Has Service

    AAV1

    Insert

    jGCaMP7c variant 1513
    

    Use

    AAV

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    Jan. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGP-CMV-GCaMP6-150

    Plasmid

    #91777

    Purpose

    Mammalian expression of low affinity calcium-sensing fluorescent protein

    Depositor

    GENIE Project, Douglas Kim

    Article

    de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

    Insert

    GCaMP6-150
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-rNFM

    Plasmid

    #32907

    Purpose

    Mammalian expression construct driving the expression of a fluorescent fusion protein consisting of EGFP linked to the N-terminus of rat neurofilament protein M

    Depositor

    Anthony Brown

    Article

    Wang et al Nat Cell Biol. 2000 Mar;2(3):137-41.

    Insert

    EGFP-rat NFM fusion (Nefm Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-synp-F-synaptophysin-GCaMP6f

    Plasmid

    #102996

    Purpose

    Expresses synaptophysin fused GCaMP6f under the synapsin promoter

    Depositor

    David Cox

    Insert

    Synaptophysin-GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBK-VQR-BE3

    Plasmid

    #85171

    Purpose

    Expresses VQR-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    VQR-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. cacnb2a (rat) in pMT2 vector

    Plasmid

    #107424

    Purpose

    Expresses Calcium channel beta2a auxillary subunit in mammalian cells

    Depositor

    Annette Dolphin

    Article

    Wyatt et al J Physiol. 1998 Jul 15;510 ( Pt 2):347-6

    Insert

    calcium channel beta2a auxillary subunit (Cacnb2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ad MLP

    Available since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-syn-synaptoPAC-minWPRE

    Plasmid

    #153085

    Purpose

    Expresses synaptoPAC, a fusion of synaptophysin, mScarlet, and bPAC in neurons

    Depositor

    Dietmar Schmitz

    Article

    Oldani et al J Neurochem. 2021 Feb;156(3):324-336. do

    Inserts

    bPAC(WT)
    Synaptophysin (Syp Rat)
    

    Use

    AAV

    Tags

    His tag, mScarlet, and myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PGK mScarlet-LC3B

    Plasmid

    #200083

    Purpose

    Mammalian expression of fluorescently-tagged marker for autophagic vesicles

    Depositor

    Erika Holzbaur

    Article

    Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

    Insert

    Map1lc3b (Map1lc3b Rat)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. pT2AL200R150G-EGFP-LC3B-RFP-LC3BΔG

    Plasmid

    #168998

    Purpose

    Expresses GFP-LC3-RFP-LC3ΔG in zebrafish to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Unspecified

    Tags

    EGFP and mRFP1

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAVTREGCaMP6f

    Plasmid

    #97410

    Purpose

    Amplified expression of GCaMP6f in combination with TET driver

    Depositor

    Tetsuo Yamamori

    Article

    Sadakane et al Cell Rep. 2015 Dec 1;13(9):1989-99. doi:

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3

    Available since

    Jan. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AAV-Syn-DIO-TRPV1-P2A-mCherry

    Plasmid

    #200831

    Purpose

    In the presence of Cre, drives neuron-specific expression of TRPV1 with fluorescence reporter mCherry

    Depositor

    Hong Chen

    Article

    Yang et al Brain Stimul. 2021 Jul-Aug;14(4):790-800

    Insert

    TRPV1-P2A-mCherry (Trpv1 Rat)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Green-Camuialpha

    Plasmid

    #26933

    Depositor

    Ryohei Yasuda

    Article

    Lee et al Nature. 2009 Mar 19. 458(7236):299-304.

    Insert

    REACh-CaMKIIa-mEGFP
    

    Use

    Tags

    REACh and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28b-BE1

    Plasmid

    #73018

    Purpose

    Expresses BE1 with N-terminal His6 tag in E. Coli

    Depositor

    David Liu

    Article

    Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

    Insert

    BE1
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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