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Showing: 601 - 650 of 664 results

  5. pZ P (cHS4)X4 OCT4p eGFP SV40pA v2

    Plasmid
    #115518
    Purpose
    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows OCT4 dependent expression of GFP, and contains cHS4 insulators.
    Depositor
    Catherine Verfaillie
    Article
    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-
    Insert
    eGFP
    Use
    AAV; Donor plasmid for recombinase-mediated casse…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    115518-plasmid-map-sequence-id-229325

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  9. pZ P TetON-3XFLAG-tdT CAGG-m2rtTA v2

    Plasmid
    #112668
    Purpose
    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression of your protein of interest.
    Depositor
    Catherine Verfaillie
    Article
    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-
    Insert
    tdT
    Use
    AAV; Donor plasmid for recombinase-mediated casse…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    112668-plasmid-map-sequence-id-229324

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  12. pYeDP60-hATP13A2-E343A

    Plasmid
    #171378
    Purpose
    A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2 E343A mutant, followed by a thrombin cleavage site and a C-term BAD tag. DOI: 10.21769/BioProtoc.3888
    Depositor
    Peter Vangheluwe
    Article
    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:
    Insert
    human ATP13A2 variant 2 wild-type
    Use
    Tags
    BAD tag
    Expression
    Yeast
    Mutation
    E343A
    Promoter
    URA3
    Available since
    Sept. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    171378-plasmid-map-sequence-id-342930

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  13. pZ:F3-P CAGGS tdTPH-F

    Plasmid
    #112667
    Purpose
    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows constitutive expression of your protein of interest.
    Depositor
    Catherine Verfaillie
    Article
    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-
    Insert
    tdT
    Use
    AAV; Donor plasmid for recombinase-mediated casse…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    112667-plasmid-map-sequence-id-229330

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  17. pABD1031

    Plasmid
    #68004
    Purpose
    Protein co-localization of PIF1 (PIL5) via fluorescent fusion after transient expression in planta
    Depositor
    Bruce Downie
    Article
    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11
    Insert
    PHY-INTERACTING FACTOR 1 (PIF1) (PIL5 )
    Use
    Tags
    eYFP
    Expression
    Plant
    Mutation
    Promoter
    2X Cauliflower Mosaic Virus 35S promoter
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  18. pABD1275

    Plasmid
    #68017
    Purpose
    PIF1 protein overproduction for purification from bacterial cells
    Depositor
    Bruce Downie
    Article
    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11
    Insert
    PHY-INTERACTING FACTOR 1 (PIF1) (PIL5 Mustard Weed, AT2G20180)
    Use
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression (GenScri…
    Promoter
    T7
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  20. pET11d-FHis-DND1-Y102A

    Plasmid
    #70082
    Purpose
    Bacterial expression of FLAG-and 6xHis-tagged human DND1 protein with defective RRM (Y102A). Codons are optimized for bacteria expression.
    Depositor
    Thomas Tuschl
    Article
    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d
    Insert
    Dnd1 (DND1 Human)
    Use
    Tags
    6xHIS and FLAG
    Expression
    Bacterial
    Mutation
    Y102A. Codon optimized for bacterial expression
    Promoter
    Available since
    Nov. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    70082-plasmid-map-sequence-id-124753

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  29. pZ P (cHS4)X4 TetON-3XFLAG-tdT CAGG-m2rtTA v2

    Plasmid
    #112669
    Purpose
    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression and contains cHS4 insulators.
    Depositor
    Catherine Verfaillie
    Article
    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-
    Insert
    tdT
    Use
    AAV; Donor plasmid for recombinase-mediated casse…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112669-plasmid-map-sequence-id-229038

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  31. pCS-TRE-FHDnd1-Ubc-rtTA-I2G

    Plasmid
    #70076
    Purpose
    2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 in mammalian cells
    Depositor
    Thomas Tuschl
    Article
    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d
    Insert
    Dnd1 (Dnd1 Mouse)
    Use
    Lentiviral
    Tags
    FLAG and HA
    Expression
    Mammalian
    Mutation
    Silent mutations are introduced to escape from RN…
    Promoter
    Tet responsible promoter
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    70076-plasmid-map-sequence-id-129464

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  32. pCS-TRE-FHDnd1-Y102A-Ubc-rtTA-I2G

    Plasmid
    #70075
    Purpose
    2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 (RRM mutant) in mammalian cells
    Depositor
    Thomas Tuschl
    Article
    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d
    Insert
    Dnd1 (Dnd1 Mouse)
    Use
    Lentiviral
    Tags
    FLAG and HA
    Expression
    Mammalian
    Mutation
    Y102A. Silent mutations are introduced to escape …
    Promoter
    Tet responsible promoter
    Available since
    Nov. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    70075-plasmid-map-sequence-id-129458

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  38. pIK86

    Plasmid
    #47933
    Purpose
    C. elegans codon-optimized Cas9 with 2xSV40 NLS sequences. It has a T7 promoter for in vitro transcription, germline-compatible 5'UTR, tbb-2 3'UTR and a polyA sequence.
    Depositor
    Iskra Katic
    Article
    Katic et al Genetics. 2013 Aug 26.
    Insert
    Cas9
    Use
    CRISPR and Unspecified
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Oct. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    47933-plasmid-map-sequence-id-68745

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  46. SP6-hCas9-Ce-mRNA

    Plasmid
    #47911
    Purpose
    In vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.
    Depositor
    Paul Sternberg
    Article
    Chiu et al Genetics. 2013 Aug 26.
    Insert
    hCas9 with C. elegans 5' and 3' UTRs
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    SP6
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    47911-plasmid-map-sequence-id-68528

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  47. pCaSpeR3/ncd-gfp #10

    Plasmid
    #66082
    Purpose
    stable germline transformation of Drosophila to produce Ncd-GFP expression
    Depositor
    Sharyn Endow
    Article
    Endow et al J Cell Biol. 1997 Jun 16;137(6):1321-36.
    Insert
    Ncd kinesin-14 motor protein (ncd Fly)
    Use
    Tags
    GFP
    Expression
    Insect
    Mutation
    D699L
    Promoter
    ncd promoter (3.1kb upstream region of ncd start …
    Available since
    June 19, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 601 - 650 of 664 results