163,606 results

1. pEF5-FRT-TagRFP-T-IFT88

   Plasmid

   #61684

   Purpose

   Mammalian expression of IFT88 fused with N terminal TagRFP.T tag

   Depositor

   Maxence Nachury

   Article

   Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

   Insert

   IFT88 (Ift88 Mouse)
   

   Use

   Tags

   TagRFP.T

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRT006.3 L1 dual-luciferase AI retrotransposition reporter

   Plasmid

   #213031

   Purpose

   Bi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integration

   Depositor

   Donna Romero, Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
   

   Use

   Luciferase

   Tags

   Firefly luciferase antisense intron (3' UTR)

   Expression

   Mammalian

   Mutation

   Promoter

   pTRE bidirectional tet-on

   Available Since

   July 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLXRN-EGFP

   Plasmid

   #99207

   Purpose

   EGFP expressing bicistronic retroviral vector

   Depositor

   Peter Pedersen

   Insert

   EGFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   A Kozak sequence was incorporated to enhance tran…

   Promoter

   LTR

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXsIP GFP-VPS33A

   Plasmid

   #67022

   Purpose

   human VPS33A ORF was inserted into pMXsIP SPGFP-GS Ci2 vector

   Depositor

   Noboru Mizushima

   Article

   Jiang et al Mol Biol Cell. 2014 Apr;25(8):1327-37. d

   Insert

   Vacuolar protein sorting 33 homolog A (S. cerevisiae) (VPS33A) (VPS33A Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pDEST47-ARL8B-GFP

   Plasmid

   #67404

   Purpose

   Mammalian expression of hArl8b with C-term GFP tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL8B (ARL8B Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. DOX_SAM_CRISPR-activation-2A-GFP-AAVS1-targeting-construct

   Plasmid

   #176836

   Purpose

   Targeting construct for intergrating a doxycycline (DOX) inducible SAM CRISPR activation complex -2A-GFP to the AAVS1 locus.

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

   Insert

   dCas9-VP64-2A-MS2-p65-HSF1-2A-GFP
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   See Depositor Comments Below

   Promoter

   Tet operator (tetracycline inducible expression)

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-Paxillin EGFP

   Plasmid

   #187688

   Purpose

   In vivo visualization of Paxillin

   Depositor

   Mary Beckerle

   Article

   Smith et al PLoS One. 2013 Aug 21;8(8):e69378. doi:

   Insert

   Paxillin (PXN Chicken)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH_SBP-EGFP-GPI

   Plasmid

   #65298

   Purpose

   synchronize trafficking of EGFP-GPI from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   GPI anchor
   

   Use

   Lentiviral

   Tags

   EGFP and IL-2 Signal Sequence and Streptavidin Bi…

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-TO-NFIA.1-SOX9 (bi-directional promoter)

   Plasmid

   #182306

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Inserts

   NFIA (NFIA Human)
   SOX9 (SOX9 Human)
   puroR-T2A-mycNLS-mTagBFP2
   

   Use

   Piggybac

   Tags

   None and T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G-Bi-directional

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. PIK3CA - Exon20 H1047R

    Plasmid

    #16639

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Science. 2004 Apr 23. 304(5670):554.

    Insert

    PIK3CA H1047R (PIK3CA Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    H1047R

    Promoter

    Available Since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. plRL58

    Plasmid

    #166886

    Purpose

    Optimized M. tuberculosis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634)

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet-On

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. MGAT-SEP-mRuby3

    Plasmid

    #200937

    Purpose

    cis/medial golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

    Depositor

    Nir Hacohen

    Article

    Liu et al Science. 2023 Aug 4;381(6657):508-514. d

    Inserts

    MGAT2 (MGAT2 Human)
    SEP-mRuby3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZ M2rtTA_CAGG TetON-Sox10

    Plasmid

    #115240

    Purpose

    RMCE donor vector for inducible expression of SOX10 constitutive expression of m2rtTA (generated from plasmid #112668)

    Depositor

    Catherine Verfaillie

    Article

    Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

    Insert

    SOX10 (SOX10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-tadDE-PcrA M6-UGI

    Plasmid

    #221236

    Purpose

    Express HACE Editor as a fusion of the engineered dual base editor TadE that can perform citosine and adenine deamination and PcrA helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    tadDE-PcrA M6-UGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiCRISPR v2-sgSLC7A11/xCT-1

    Plasmid

    #161818

    Purpose

    knock out SLC7A11/xCT in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

    Insert

    SLC7A11 solute carrier family 7 member 11 xCT (SLC7A11 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. PPRE-pNL1.3

    Plasmid

    #84394

    Purpose

    Secreted Luciferase reporter for PPARgamma activity

    Depositor

    Thierry Fournier, Severine Degrelle

    Article

    Degrelle et al PPAR Res. 2017;2017:6139107. doi: 10.115

    Insert

    PPARG response element (PPARG Human)
    

    Use

    Tags

    secNluc

    Expression

    Mammalian

    Mutation

    Promoter

    PPRE

    Available Since

    Jan. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. MiniCoopR mitfa:EGFP

    Plasmid

    #118850

    Purpose

    Expresses EGFP and zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    mitfa: EGFP (mitfa Synthetic, Zebrafish)
    

    Use

    Zebrafish expression, tol6

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)

    Plasmid

    #225755

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detection

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBABE-neo largeTcDNA

    Plasmid

    #1780

    Purpose

    Retroviral expression of Large T Antigen. Used to create immortalized cell lines.

    Depositor

    Bob Weinberg

    Article

    Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

    Insert

    large T antigen (SV40gp6 simian virus)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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20. FKBP-C-TEV 219

    Plasmid

    #100096

    Purpose

    SNIPer plasmid, C terminal TEV fused to FKBP

    Depositor

    Jim Wells

    Article

    Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

    Insert

    C-TEV
    

    Use

    Retroviral

    Tags

    FKBP and Myc

    Expression

    Mammalian

    Mutation

    C terminal domain

    Promoter

    CMV

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. LRG2.1-BFP

    Plasmid

    #120577

    Purpose

    Lentiviral plasmid to co-express a guide RNA and EBFP.

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBHM2329

    Plasmid

    #171686

    Purpose

    pRS316-PCnU6-scaffold, contains template for amplification of PCnU6 and scaffold for fusion PCR

    Depositor

    Hiten Madhani

    Article

    Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

    Insert

    PCnU6-sgRNA_scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    C. neoformans U6

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHAGE-IRES-puro-NLS-dPspCas13b-3xEGFP-NLS-3xFlag

    Plasmid

    #132398

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPspCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    H133A; H1059A

    Promoter

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pmCherry-C1 mCherry-NLS

    Plasmid

    #58476

    Purpose

    Expresses a nuclear-targeted mCherry control construct, on a CMV reporter

    Depositor

    Dyche Mullins

    Article

    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

    Insert

    3X NLS with 3X stop codons
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. human p53-(82-393)

    Plasmid

    #24867

    Depositor

    Cheryl Arrowsmith

    Article

    Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 82-393

    Promoter

    Available Since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

26. pAAV-mDlx-GFP-Fishell-1

    Plasmid

    #83900

    Purpose

    GFP expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

    Depositor

    Gordon Fishell

    Article

    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    mDlx

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pKM468-EGFP

    Plasmid

    #108434

    Purpose

    ORBIT integrating plasmid for C-terminal tagging with EGFP.

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Insert

    attB-eGFP-4xGly-TEV-Flag-6xHis
    

    Use

    Orbit integrating plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. lentiCRISPR v2-sgSLC7A11/xCT-2

    Plasmid

    #161819

    Purpose

    knock out SLC7A11/xCT in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

    Insert

    solute carrier family 7 member 11 (SLC7A11 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLV-hCDC6p-Venus-puro

    Plasmid

    #212666

    Purpose

    Express Venus under CDC6 promoter

    Depositor

    Yumi Konagaya, Tobias Meyer

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    hCDC6p-Venus
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hCDC6p

    Available Since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMVHA hEZH2

    Plasmid

    #24230

    Purpose

    Mammalian expression of human EZH2 with HA tag

    Depositor

    Kristian Helin

    Article

    Bracken et al EMBO J. 2003 Oct 15. 22(20):5323-35.

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge