Addgene

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ptdEos-CLC

Plasmid

#38009

Depositor

Xiaowei Zhuang

Article

Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

Inserts

Clathrin, light polypeptide (LCa) (Clta Mouse)
Eos FP, tandem dimer

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant

Plasmid

#87350

Purpose

S867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET construct

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Use

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/S355C/C574S/S867C

Promoter

T7

Available since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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SPDK3860

Plasmid

#199245

Purpose

Modified Tobacco rattle virus (TRV) RNA2 vector for editing of Nicotiana benthamiana Phytoene desaturase (PDS) gene

Depositor

Savithramma Dinesh-Kumar

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence

Use

T-dna vector

Tags

Expression

Mutation

Promoter

pPEBV

Available since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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pKMD106h - intein-Cas9(C574)

Plasmid

#64191

Purpose

Expresses intein-Cas9(C574) in mammalian cells

Depositor

David Liu

Article

Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

Insert

mammalian codon-optimized S. pyogenes intein-Cas9(C574) - NLS - 3x FLAG

Use

CRISPR

Tags

NLS - 3x FLAG (C terminal on insert)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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Nano-lantern-plasma membrane/pcDNA3

Plasmid

#51975

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to the plasma membrane

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Use

Tags

HIS and Lyn

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pJT11

Plasmid

#98308

Purpose

Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Citrus limon limonene synthase LIS1 under the control of GAL1/GAL2 promoter

Depositor

Claudia Vickers

Article

Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

Insert

TRPL3ClLIS1>TRPL41B

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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TB:6xAP-1_RE:MiniP

Plasmid

#133884

Purpose

6 copies of the AP-1 binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

6 copies of the AP-1 binding motif

Use

Luciferase and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pP19 (GB0038)

Plasmid

#68195

Purpose

Provides the CDS of the Tomato bushy stunt virus (TBSV) silencing suppressor P19 as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

P19 coding region

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCfB3034(X-3 MarkerFree)

Plasmid

#73272

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-3 (Chr X: 223616..224744)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR221-PGK.Pro

Plasmid

#79489

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human PGK promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

PGK Promoter (PGK1 Human, Synthetic)

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

PGK

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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Orange Nano-lantern/pRSETB

Plasmid

#67659

Purpose

orange luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Orange Nano-lantern

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pU6-26 (GB1001)

Plasmid

#68208

Purpose

Provides the A. thaliana U6-26 RNA polIII promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtU6-26 promoter

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pYES260-sc.eEF2

Plasmid

#85119

Purpose

yeast expression of EEF2

Depositor

Pal Falnes

Article

Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

Insert

EFT1/EFT2 (EFT2, EFT1 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pEGB Tnos:NptII:Pnos (GB0226)

Plasmid

#68212

Purpose

Transcriptional unit for kanamycin resistance gene plant expression

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

NptII

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos

Available since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

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GOMO-GFP-LUC

Plasmid

#60976

Purpose

Rapid selection of recombinant marker-free bioluminescent rodent malaria parasites

Depositor

Olivier Silvie

Article

Manzoni et al Sci Rep. 2014 Apr 23;4:4760. doi: 10.103

Inserts

GFP-Luciferase fusion
mCherry
hDHFR-yFCU

Use

Tags

Expression

Mutation

Promoter

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pHsp18.2 (GB0153)

Plasmid

#68171

Purpose

Provides the Arabidopsis thaliana heat shock protein 18.2 promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtHsp18.2 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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Cyan Nano-lantern/pRSETB

Plasmid

#67658

Purpose

cyan luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Cyan Nano-lantern

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pHygroR-GMRWhite

Plasmid

#165886

Purpose

Hygromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Transgenesis Ready Selection Vector

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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Cyan Nano-lantern(Ca2+)/pcDNA3

Plasmid

#71344

Purpose

Express Cyan Nano-lantern-based luminescent Ca2+ indicator in mammalian cell

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Cyan Nano-lantern(Ca2+)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only
pCfB2899(X-2 MarkerFree)

Plasmid

#73271

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-2 (Chr X: 194944..195980)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pHsp70B (GB0155)

Plasmid

#68172

Purpose

Provides the Arabidopsis thaliana heat shock protein 70B promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtHsp70B promoter

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pDelila (GB0079)

Plasmid

#68200

Purpose

Provides the CDS of the Antirrhinum majus Delila transcription factor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Delila coding region

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pRosea (GB0026)

Plasmid

#68194

Purpose

Provides the CDS of the Antirrhinum majus Rosea transcription factor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

A. majus Rosea coding region

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-ΔD-box-CycA-GFP (525)

Plasmid

#39856

Depositor

Jonathon Pines

Article

den Elzen et al J Cell Biol. 2001 Apr 2;153(1):121-36.

Insert

Cyclin A2 (CCNA2 Human)

Use

Tags

GFP and Myc

Expression

Mammalian

Mutation

L2V; Δ destruction box = deletion of aa47-55

Promoter

Available since

Jan. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pRenilla (GB0056)

Plasmid

#68198

Purpose

Provides the CDS of the Renilla protein as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Renilla coding region

Use

Luciferase and Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-GEM

Plasmid

#111191

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Piddini et al EMBO J. 2001 Aug 1. 20(15):4076-87.

Insert

GEM (GEM Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

T2A

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pBI101.1/ProGOXL1:GUS

Plasmid

#175557

Purpose

Binary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genes

Depositor

George Haughn

Article

Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

Insert

ProGOXL1

Use

Tags

GUS

Expression

Plant

Mutation

Promoter

GOXL1

Available since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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CLM RP HTR

Plasmid

#24430

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Use

Tags

6XHis

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S/C421A

Promoter

Available since

June 30, 2010

Availability

Academic Institutions and Nonprofits only
pEYFP-Pds1Δdestruction box(383)

Plasmid

#39848

Depositor

Jonathon Pines

Article

Hagting et al J Cell Biol. 2002 Jun 24;157(7):1125-37.

Insert

Pds1 (PTTG1 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Δdestruction box = ΔR61-N68

Promoter

Available since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only

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MC177VSG221RNAi

Plasmid

#59730

Purpose

For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.

Depositor

Gloria Rudenko

Article

Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10

Insert

VSG221 RNAi fragment

Use

RNAi

Tags

Expression

Mutation

Promoter

opposing T7 promoters

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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