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pBB540

Plasmid

#27393

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

grpE, clpB

Expression

Bacterial

Available Since

April 26, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Peredox-mCherry-NLS

Plasmid

#32384

Depositor

Gary Yellen

Article

Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

Insert

Peredox-mCherry-NLS

Tags

Nuclear Localization Seq

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pLKO-PTEN-shRNA-1320

Plasmid

#25638

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

Insert

PTEN-shRNA-1320 (PTEN Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-300(HAT-)

Plasmid

#23254

Depositor

Warner Greene

Article

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

p300 (EP300 Human)

Expression

Mammalian

Mutation

H1415A E1423A Y1424A L1428S Y1430A H1434A

Available Since

March 15, 2010

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP30

Plasmid

#22578

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP30 (USP30 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO Puro empty (w175-1)

Plasmid

#17482

Purpose

3rd gen lentiviral empty control vector, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pSIN4-EF2-N2L

Plasmid

#21163

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Nanog, Lin28 (NANOG, LIN28A Human)

Use

Lentiviral; Reprogramming

Expression

Mammalian

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-rev-ChR2(H134R)-mCherry

Plasmid

#18916

Purpose

Cre-dependent viral expression of ChR2(H134R)-mCherry for neuronal excitation

Depositor

Scott Sternson

Article

Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

Insert

Channelrhodopsin 2-mCherry

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

mCherry

Mutation

H134R

Promoter

CAG

Available Since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only

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pJL44 - Phsp16.48::MosTase::glh-2utr

Plasmid

#19333

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Phsp-16::Mos1 transposase

Expression

Worm

Available Since

Oct. 29, 2008

Availability

Academic Institutions and Nonprofits only

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CBFRE-EGFP

Plasmid

#17705

Depositor

Nicholas Gaiano

Article

Mizutani et al Nature. 2007 Sep 20. 449(7160):351-5.

Insert

CBFRE

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 beta-catenin WT

Plasmid

#16071

Depositor

Erin Schuman

Article

Murase et al Neuron. 2002 Jul 3. 35(1):91-105.

Insert

beta-catenin (CTNNB1 Chicken)

Tags

EGFP

Expression

Mammalian

Mutation

*

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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3xIRS luciferase

Plasmid

#13511

Depositor

Kunliang Guan

Article

Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

Insert

3xIRS (IGFBP1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Dec. 11, 2006

Availability

Academic Institutions and Nonprofits only

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p3113 GST-p53

Plasmid

#10852

Depositor

Peter Howley

Article

Huibregtse et al EMBO J. 1991 Dec . 10(13):4129-35.

Insert

p53 (TP53 Human)

Tags

GST

Expression

Bacterial

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pcdna3.1 hciap1

Plasmid

#8311

Depositor

Jon Ashwell

Article

Li et al Nature 2002 Mar 21;416(6878):345-7.

Insert

cIAP1 (BIRC2 Human)

Tags

myc

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pDY0423 Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)

Plasmid

#179116

Purpose

Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

Cargo EGFP with AttP Bxb1 site for in-frame fusion

Use

CRISPR

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G

Plasmid

#167935

Purpose

Inducible CRISPRi expression

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

KRAB-dCas9-DHFR

Use

Lentiviral

Promoter

tetON

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pT3TS-RfxCas13d-HA

Plasmid

#141320

Purpose

Plasmid to carry out IVT of RfxCas13d (human codon-optimized)

Depositor

Ariel Bazzini, Miguel Angel Moreno-Mateos

Article

Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

Insert

Rfx-Cas13d

Use

CRISPR

Tags

HA

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLENTI_hACE2_PURO

Plasmid

#155295

Purpose

Lentiviral vector to generate hACE2 stable expressing cell line

Depositor

Raffaele De Francesco

Insert

hACE2 (ACE2 Human)

Use

Lentiviral

Tags

C9

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-EF1alpha-SARS-CoV-2-nsp12-2xStrep-IRES-Puro

Plasmid

#141378

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp12 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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LentiGuide Puro-P2A-EGFP

Plasmid

#137729

Purpose

For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).

Depositor

Fredrik Wermeling

Article

Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

Type

Empty backbone

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

EGFP

Expression

Bacterial and Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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