We narrowed to 11,551 results for: NSI

1. pGL-p21UTR

   Plasmid

   #20014

   Depositor

   Robert Blelloch

   Article

   Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

   Insert

   p21 3'UTR (Cdkn1a Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

2. Integrin beta1-tev-BHCys

   Plasmid

   #27314

   Depositor

   Timothy Springer

   Article

   Takagi et al Nat Struct Biol. 2001 May . 8(5):412-6.

   Insert

   integrin beta1 (ITGB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   integrin beta1(1-708)+GG+TEV+GGKN+BHCys

   Promoter

   Available Since

   April 8, 2011

   Availability

   Academic Institutions and Nonprofits only

3. MCP-ADAR2-DD(E488Q)

   Plasmid

   #170125

   Purpose

   Lentiviral vector carrying the MCP-ADAR2(E488Q) with a nuclear export signal

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   MCP-ADAR2-DD(E488Q)-NES
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a core promoter

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCB163

   Plasmid

   #37388

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

   Insert

   Dynactin 2 (DCTN2 Human)
   

   Use

   Retroviral

   Tags

   GFP, S-tag, and TEV

   Expression

   Mammalian

   Mutation

   A7P

   Promoter

   Available Since

   July 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. LCV2 control

   Plasmid

   #217443

   Purpose

   Lentiviral vector expressing Cas9 without a targeting sgRNA

   Depositor

   Jarrod Johnson

   Article

   Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SStart-36xMashTag-BFP-3xStop-24xPP7 (Sunstart-MashTag reporter)

   Plasmid

   #128608

   Purpose

   Expresses 36xMashTag with SunTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

   Depositor

   Marvin Tanenbaum

   Article

   Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

   Insert

   36xMashTag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Pcmv

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAVS1-Nst-CAG-DEST

   Plasmid

   #80489

   Purpose

   Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Type

   Empty backbone

   Use

   Donor vector (human)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET_RGG-GFP-RGG

   Plasmid

   #124948

   Purpose

   Expression of RGG-GFP-RGG

   Depositor

   Benjamin Schuster, Daniel Hammer, Matthew Good

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
   

   Use

   Tags

   EGFP and His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLentiCRISPR_zeo_sgNT135

   Plasmid

   #183181

   Purpose

   pLentiCRISPR that is selectable with zeocin with a non-targeting sgRNA

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   NT135
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYPQAT27

    Plasmid

    #223399

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by 2x35s and the sgRNA was driven by AtU3; Kanamycin for plant select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYPQAT13

    Plasmid

    #223385

    Purpose

    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    AtUBQ10-hA3A-Y130F-SpCas9-D10A-UGI-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTRIPZ-EGFP:Ajuba

    Plasmid

    #108229

    Purpose

    Lentiviral vector for Tet-inducible Ajuba expression

    Depositor

    Kenneth Irvine

    Article

    Ibar et al J Cell Sci. 2018 Feb 13. pii: jcs.214700

    Insert

    AJUBA (AJUBA Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Tet/ON Minimal CMV promoter

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pARF1(Q71L)-CFP

    Plasmid

    #11385

    Depositor

    Joel Swanson

    Article

    Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

    Insert

    ADP Ribosylation Factor 1 (ARF1 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Changed Glutamine 71 to Leucine.

    Promoter

    Available Since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pARF1(T31N)-CFP

    Plasmid

    #11384

    Depositor

    Joel Swanson

    Article

    Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

    Insert

    ADP Ribosylation Factor 1 (ARF1 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Changed Threonine 31 to Asparagine.

    Promoter

    Available Since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pARF1-CFP

    Plasmid

    #11381

    Depositor

    Joel Swanson

    Article

    Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

    Insert

    ADP Ribosylation Factor 1 (ARF1 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGG-cTP-TurboID

    Plasmid

    #209394

    Purpose

    Transiently expressing TurboID fused with a chloroplast transit peptide (cTP) in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    cTP-3XHA-TurboID
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available Since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCS2 GFP-GSK3-MAPK

    Plasmid

    #29689

    Purpose

    GFP reporter protein fused to three GSK3 phosphorylation sites

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    GFP glycogen synthase kinase 3 recognition site primed by MAPK
    

    Use

    Tags

    Flag and Flag-EGFP

    Expression

    Mammalian

    Mutation

    recognition sites only, and E3 ligase site

    Promoter

    SP6

    Available Since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPML1 (GB0045)

    Plasmid

    #68164

    Purpose

    Provides the Arabidopsis thaliana Meristem Layer 1 promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtML1 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJE53

    Plasmid

    #194153

    Purpose

    sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, KmR. Contains mrfp nontargeting control guide sequence.

    Depositor

    Edward Geisinger

    Article

    Bai et al J Bacteriol. 2021 May 20;203(12):e005652

    Insert

    sgRNA with mrfp control guide sequence
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. raACR-TS-Kv-P2A-mCerulean3-pAAV-CaMKII

    Plasmid

    #204971

    Purpose

    Soma-targeted neuronal expression of the MsACR1 mutant raACR and mCerulean3 separated by a P2A site.

    Depositor

    Peter Hegemann

    Article

    Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

    Insert

    raACR
    

    Use

    AAV

    Tags

    mCerulean3

    Expression

    Mutation

    Promoter

    CaMKII

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEF.DEST51-mScarlet

    Plasmid

    #207960

    Purpose

    Vector for overexpression of the mScarlet fluorescent protein

    Depositor

    Johanna Ivaska

    Article

    Conway et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

    Insert

    mScarlet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pYPQAT25

    Plasmid

    #223397

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by AtUBQ10 and sgRNA was driven by AtU3; Hygromycin for plants select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    AtUBQ10-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-PB2-B/BRISBANE/60/2008

    Plasmid

    #187917

    Purpose

    Expresses PB2 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    polymerase basic protein 2
    

    Use

    Tags

    UNTAGGED

    Expression

    Mutation

    Promoter

    CMV Promoter

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEGB 35S:Luciferase:Tnos (GB0110)

    Plasmid

    #68216

    Purpose

    Transcriptional unit for Firefly Luciferase Protein plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Luc
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAGGS_cAMPFIRE-L-R279E

    Plasmid

    #182286

    Purpose

    Mammalian expression of cAMPFIRE-L-R279E (non-cAMP-binding) under a CAG promotor w/ WPRE translation enhancer.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCDNA3_cAMPFIRE-L-R279E

    Plasmid

    #182282

    Purpose

    Mammalian expression of cAMPFIRE-L with R279E (non-cAMP-binding) mutation under a CMV promotor.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRS416 Gal TDP43 WT

    Plasmid

    #27458

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

28. pCTCON2 Aga2P-HRP

    Plasmid

    #73152

    Purpose

    Full-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    Aga2P-HRP
    

    Use

    Tags

    HA tag and myc tag

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MsACR1-TS-Kv-P2A-mCerulean3-pAAV-CaMKII

    Plasmid

    #204970

    Purpose

    Soma-targeted neuronal expression of MsACR1 and mCerulean3 separated by a P2A site.

    Depositor

    Peter Hegemann

    Article

    Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

    Insert

    MsACR1
    

    Use

    AAV

    Tags

    mCerulean3

    Expression

    Mutation

    Promoter

    CaMKII

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLKO.1 puro_shTBK1_3185

    Plasmid

    #167294

    Purpose

    Lentiviral plKO.1 construct coding for a short-hairpin RNA targeting the human gene TBK1. Contains a puro resistance cassette.

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    shRNA TBK1 (TBK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3-sapphireCKAR

    Plasmid

    #118468

    Purpose

    cp-T-sapphire-based single-color biosensor for monitoring Protein Kinase C activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireCKAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV-TRE-DIO-ChR2-TdTomato (Cre-OFF)

    Plasmid

    #166610

    Purpose

    Encodes Cre-inactivated ChR2-TdTomato under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    ChR2-TdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    H134R

    Promoter

    tetracycline response element

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3-RAB-AKARev

    Plasmid

    #118478

    Purpose

    ddRFP-based single-color biosensor for monitoring PKA (Protein Kinase A) activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    RAB-AKARev
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. FUGW-Golgi-TurboID

    Plasmid

    #200964

    Purpose

    3rd gen lentiviral plasmid for expressing golgi localized TurboID with EGFP

    Depositor

    Keriann Backus

    Article

    Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

    Insert

    Golgi-TurboID
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. 3xFLAG-SENP7

    Plasmid

    #42886

    Depositor

    Edward Yeh

    Article

    Bawa-Khalfe et al Proc Natl Acad Sci U S A. 2012 Oct 23;10

    Insert

    SENP7 (SENP7 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    T102A, R969C, L996S

    Promoter

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. UPB-stomach (UPB1)

    Plasmid

    #180784

    Purpose

    Unique Projection Barcode (UPB1)-Stomach for Projection-seq

    Depositor

    Rui Chang

    Article

    Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

    Insert

    humanized ChR2-H134R (partial)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV-nef-CIAO2-TVA-mCherry

    Plasmid

    #149297

    Purpose

    Cre-dependent expression of TVA:mCherry fusion protein for rabies tracing.

    Depositor

    Edward Callaway

    Article

    Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

    Insert

    TVA:mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Start codon removed.

    Promoter

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSMAL

    Plasmid

    #161785

    Purpose

    Gateway destination vector with bidirectional CMV-SFFV promoter for high expression of gene of interest and GFP.

    Depositor

    Peter van Galen, John Dick

    Article

    van Galen et al Cell Stem Cell. 2014 Jan 2;14(1):94-106.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Bidirectional minhCMV and SFFV

    Available Since

    Dec. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. 2Bc-T MBP_BoMoC(ed)_F753A_6xH

    Plasmid

    #185710

    Purpose

    Recombinant B. mori truncated R2 RT expression

    Depositor

    Kathleen Collins

    Article

    Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

    Insert

    B. mori truncated R2 RT
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Changed F753 to A

    Promoter

    T7

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. UPB-lung (UPB2)

    Plasmid

    #180788

    Purpose

    Unique Projection Barcode (UPB2)-Lung for Projection-seq

    Depositor

    Rui Chang

    Article

    Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

    Insert

    humanized ChR2-H134R (partial)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge