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We narrowed to 7,780 results for: amph

Showing: 6501 - 6550 of 7780 results

  3. pALS099 (pGEX-2TP-cTevHis-DEST)

    Plasmid
    #139787
    Purpose
    Gateway destination vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHis
    Depositor
    Jeannie Lee, Arneet Saltzman
    Article
    Saltzman et al Genetics. 2018 Nov;210(3):907-923. doi:
    Type
    Empty backbone
    Use
    Tags
    6xHis (cleavable by TEV protease) and GST (cleava…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    139787-plasmid-map-sequence-id-325052

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  6. pMpGWB114

    Plasmid
    #68568
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Use
    Tags
    Glucocorticoid receptor (GR)
    Expression
    Plant
    Mutation
    Promoter
    Cauliflower mosaic virus 35S promoter
    Available since
    July 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    68568-plasmid-map-sequence-id-121730

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  8. pFLAG_attP

    Plasmid
    #110095
    Purpose
    Vector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Use
    Tags
    3xFLAG
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110095-plasmid-map-sequence-id-317064

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  10. pGBW-m4046757

    Plasmid
    #145657
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145657-plasmid-map-sequence-id-298331

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  16. pLEG(R1-R3) #BGV149

    Plasmid
    #48956
    Purpose
    Lentiviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).
    Depositor
    David Dankort
    Article
    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. pMK-Cas9-gate

    Plasmid
    #113743
    Purpose
    Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving aminoglycoside phosphotransferase for G418 selection in plants.
    Depositor
    Magdalena Bezanilla
    Article
    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d
    Insert
    Cas9
    Use
    CRISPR; Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Z. Mays ubiquitin promoter, 35S promoter
    Available since
    Sept. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    113743-plasmid-map-sequence-id-220965

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  21. pJK511

    Plasmid
    #155384
    Purpose
    dCas12a oscillator, reverse direction crRNAs. dCas12a (D917A) + PA4-mVenus
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Promoter
    Available since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    155384-plasmid-map-sequence-id-309004

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  23. pLPP5

    Plasmid
    #209967
    Purpose
    Contains Level 0 Part: inducible Promoter (PsppQ) with two regulatory genes sppK and sppR for the construction of Level 1 plasmids
    Depositor
    Thorsten Mascher
    Article
    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2
    Insert
    Promoter (P_sppQ)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 13, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    209967-plasmid-map-sequence-id-419962

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  27. pDGE657

    Plasmid
    #153255
    Purpose
    M2E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
    Depositor
    Johannes Stuttmann
    Article
    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    153255-plasmid-map-sequence-id-305906

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  32. pGLTR-X-PURO

    Plasmid
    #58246
    Purpose
    GATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and Puromycin selection
    Depositor
    Stephan Geley
    Article
    Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:
    Insert
    Puro(R)
    Use
    Lentiviral and RNAi; Gateway destionation vector
    Tags
    TetR-NLS-T2A
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58246-plasmid-map-sequence-id-89671

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  36. M13cp-dg1 hp

    Plasmid
    #218093
    Purpose
    Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pI
    Depositor
    Kevin Esvelt
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    218093-plasmid-map-sequence-id-432465

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  37. pCB-CBEv2

    Plasmid
    #221139
    Purpose
    Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 2 without sgRNA construct. Expresses 3xF-BE4-PpAPOBEC1(H122A).
    Depositor
    Craig Roy
    Article
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    3xF-PpAPOBEC1(H122A)-Cas9n-UGI-UGI (BE4-PpAPOBEC1(H122A))
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Bacterial
    Mutation
    Promoter
    lacIq-Ptac
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221139-plasmid-map-sequence-id-437031

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  39. pACM4-ACA

    Plasmid
    #49809
    Purpose
    Encodes fabD, accA, accB, accC, accD in pseudo-operon configuration.
    Depositor
    Mattheos Koffas
    Article
    Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc
    Inserts
    ACP transacylase
    Acetyl-CoA carboxylase
    acetyl-CoA carboxylase
    Acetyl-CoA carboxylase
    Acetyl-CoA carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Silent site-directed mutagenesis to remove SalI s…
    Promoter
    T7
    Available since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    49809-plasmid-map-sequence-id-74624

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  40. PROM_MpUBE2

    Plasmid
    #136109
    Purpose
    PROM for cloning with 5UTR_MpUBE2. PROM+5UTR_MpUBE2 has ubiquitous expression in early satges of gemmae development.
    Depositor
    Jim Haseloff
    Article
    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102
    Insert
    PROM_MpUBE2 Mapoly0106s0019
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI/ SapI domesticated
    Promoter
    Available since
    Nov. 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    136109-plasmid-map-sequence-id-275127

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  41. 5UTR_MpUBE2

    Plasmid
    #136110
    Purpose
    5UTR for cloning with PROM_MpUEB2. PROM+5UTR_MpUBE2 has ubiquitous expression in early stages of gemmae development.
    Depositor
    Jim Haseloff
    Article
    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102
    Insert
    5UTR_MpUBE2 Mapoly0106s0019
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI/ SapI domesticated
    Promoter
    Available since
    Nov. 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    136110-plasmid-map-sequence-id-275128

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  42. pDGE479

    Plasmid
    #153270
    Purpose
    M4E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
    Depositor
    Johannes Stuttmann
    Article
    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    153270-plasmid-map-sequence-id-307108

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  44. pLEG(R1-R4) #BGV057

    Plasmid
    #48957
    Purpose
    Lentiviral Destination vector containing AttR1-AttR4 site. Use with 4-way LR reaction with cDNA in entry vector, marker in pBEG R2-L3 plasmids and shRNA in pBEG R3-L4 plasmid (module 2,3,and 4)
    Depositor
    David Dankort
    Article
    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  46. pASKt15C+SrtA7

    Plasmid
    #64986
    Purpose
    Expresses the P94R/E105K/E108A/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/
    Insert
    sortase A
    Use
    Tags
    His6 tag and S tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-59, P94R, E105K, E108A, D16…
    Promoter
    tetracycline promoter/operator
    Available since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    64986-plasmid-map-sequence-id-109956

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  50. pJAK080

    Plasmid
    #167279
    Purpose
    CodA-based vector for insertion of DNA by recombination downstream of the pyrE gene in C. difficile
    Depositor
    Robert Fagan
    Article
    Fuchs et al Proc Natl Acad Sci U S A. 2021 Jun 22;11
    Type
    Empty backbone
    Use
    E. coli - c. difficile shuttle vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    167279-plasmid-map-sequence-id-330742

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Showing: 6501 - 6550 of 7780 results