We narrowed to 16,109 results for: form

1. pLib6.4B-Cquin_596

   Plasmid

   #176669

   Purpose

   Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039596) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Expression

   Insect

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. C. difficile toxin B mRNA toehold switch sensor

   Plasmid

   #110715

   Purpose

   Toehold switch sensor to detect the tcdB mRNA from toxigenic C. difficile with GFP output

   Depositor

   James Collins

   Article

   Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

   Insert

   C. difficile toxin B toehold switch sensor
   

   Use

   Synthetic Biology

   Promoter

   T7

   Available Since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGWB427

   Plasmid

   #74821

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with T7 (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGWB426

   Plasmid

   #74820

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with T7 (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBGSA c-myc-FPR1

   Plasmid

   #62601

   Purpose

   Constitutive expression of c-myc-FPR1 in mammalian cells

   Depositor

   Heini Miettinen

   Article

   Gripentrog et al J Immunol. 2003 Sep 15;171(6):3187-93.

   Insert

   FPR1 (FPR1 Human)
   

   Tags

   c-myc

   Expression

   Mammalian

   Promoter

   SRalpha

   Available Since

   Feb. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Flag-ATXN1_L588D

   Plasmid

   #48194

   Purpose

   Expresses L588D mutated Ataxin1

   Depositor

   Huda Zoghbi

   Article

   Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

   Insert

   Ataxin1 (ATXN1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Flag-ATXN1_L588A

   Plasmid

   #48193

   Purpose

   Expresses L588A mutated Ataxin1

   Depositor

   Huda Zoghbi

   Article

   Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

   Insert

   Ataxin1 (ATXN1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Flag-ATXN1_R665A

   Plasmid

   #48195

   Purpose

   Expresses R665A mutated Ataxin1

   Depositor

   Huda Zoghbi

   Article

   Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

   Insert

   Ataxin1 (ATXN1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJEC626

   Plasmid

   #174367

   Purpose

   Activation domain for modular CRISPRa. AD-SYNZIP. Expresses alphaNTD from the E coli RNAP with a SYNZIP17 domain fused to the N terminus.

   Depositor

   James Chappell

   Article

   Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

   Insert

   Alpha N-terminal domain from the E. coli RNA polymerase fused to SYNZIP17
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23106

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. hNedd4-2 CS

    Plasmid

    #86041

    Purpose

    full length NeddL3 with C to S mutation with His Xpress tag

    Depositor

    Christie Thomas

    Article

    Raikwar et al Am J Physiol Renal Physiol. 2010 Aug;299

    Insert

    hNeddL3 with C to S mutation in HECT domain (NEDD4 Human)
    

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    C942S mutation in HECT domain

    Promoter

    CMV

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. A337C

    Plasmid

    #24432

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S/C421A, A337C

    Available Since

    June 14, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pCMV-MA-VN

    Plasmid

    #123283

    Purpose

    Mammalian expression plasmid for matrix domain of HIV-1 Gag fused to the N-terminal half of split fluorescent Venus (VN)

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    Matrix
    

    Tags

    VN: N-terminus of split Venus (a.a. V1–A154) I152…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGSTag Flag p38 gamma

    Plasmid

    #20818

    Depositor

    Roger Davis

    Article

    Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

    Insert

    pGSTag Flag p38 gamma (Mapk12 Rat)
    

    Tags

    Flag

    Expression

    Bacterial

    Available Since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pCellFree_G03 FYN

    Plasmid

    #67106

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    FYN (FYN Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

15. p63RhoGEF580-mVenus-N1

    Plasmid

    #84338

    Purpose

    Expresses p63 (580 a.a.) tagged with mVenus

    Depositor

    Dorus Gadella

    Article

    van Unen et al Sci Rep. 2016 Nov 11;6:36825. doi: 10.10

    Insert

    p63RhoGEF (580 aa) (ARHGEF25 Human)
    

    Tags

    mVenus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLyGo-Kp-2

    Plasmid

    #163144

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-amylase signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. XE158 GFP-delta LZ-XDpr-CS2+

    Plasmid

    #16764

    Depositor

    Randall Moon

    Article

    Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

    Insert

    delta LZ-XDpr (dact1-b Frog)
    

    Use

    Xenopus expression

    Tags

    gfp

    Mutation

    XDpr is truncated to delete the first 129 amino a…

    Available Since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pProvector_AAV2_586_PLGLAR

    Plasmid

    #129024

    Purpose

    Recombinant AAV2 rep/cap plasmid modified to include an 18 amino acid peptide at cap position 586.

    Depositor

    Junghae Suh

    Article

    Tong et al J Control Release. 2019 Jun 25. pii: S01

    Insert

    Rep2/Cap2 with PLGLAR lock at 586
    

    Use

    AAV

    Mutation

    inserted peptide motif (PLGLARGDDDDGPLGLAR) at po…

    Available Since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGWB422

    Plasmid

    #74816

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with GST (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. actc1b-mKate2-LactC2

    Plasmid

    #109499

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to the C-terminal domain from cow (Bos taurus) milk fat globue EGF-factor 8 protein (MFGE8). Binds phosphatidylserine (PS)

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    actc1b-mKate2-LactC2
    

    Use

    Zebrafish plasmids

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGWB428

    Plasmid

    #74822

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with TAP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-CaMKII-Mac-GFP

    Plasmid

    #58849

    Purpose

    AAV expression of Mac-GFP under the CaMKII promoter

    Depositor

    Edward Boyden

    Article

    Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

    Insert

    Mac-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CaMKII

    Available Since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mSTIM1_1-310-CFP

    Plasmid

    #69551

    Purpose

    Expresses a STIM1_1-310 fragment mammalian cells

    Depositor

    Yubin Zhou

    Article

    Ma et al Nat Commun. 2015 Jul 17;6:7826. doi: 10.

    Insert

    mSTIM1_1-310
    

    Tags

    Cyan Fluorescent Protein (CFP)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pLenti CRISPR sgSHMT1_2

    Plasmid

    #106312

    Purpose

    Express Cas9 and sgRNA targeting SHMT1

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting SHMT1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. actc1b-mKate2-rab7a

    Plasmid

    #109655

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to rab7a

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab7a (rab7a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. actc1b-mKate2-rab7b

    Plasmid

    #109656

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to rab7b

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab7b (rab7b Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCellFree_G03 CREBZF

    Plasmid

    #67070

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CREBZF (CREBZF Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

28. LC402: pMVP (L3-L2) eGFP + polyA

    Plasmid

    #121763

    Purpose

    pMVP L3-L2 entry plasmid, contains eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eGFP fusion to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    eGFP + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. KG802: pMVP (L3-L2) V5 epitope tag + polyA

    Plasmid

    #121755

    Purpose

    pMVP L3-L2 entry plasmid, contains V5 epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    V5 epitope tag + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGex-3x Flag MKK3

    Plasmid

    #14814

    Depositor

    Roger Davis

    Article

    Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

    Insert

    MKK3 (MAP2K3 Human)
    

    Tags

    Flag

    Expression

    Bacterial

    Available Since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. rGSKmyc-R85-pXT7

    Plasmid

    #15493

    Depositor

    Sergei Sokol

    Article

    Dominguez et al Proc Natl Acad Sci U S A. 1995 Aug 29. 9

    Insert

    GSK-3 beta R85 (Gsk3b Rat)
    

    Use

    Xenopus rna expression

    Tags

    myc

    Mutation

    R85: K85R, dominant negative

    Available Since

    Aug. 1, 2007

    Availability

    Academic Institutions and Nonprofits only

32. actc1b-mKate2-rab8a

    Plasmid

    #109657

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to rab8a

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab8a (rab8a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLib6.4-Aalb_726

    Plasmid

    #176661

    Purpose

    Expression of sgRNA under Ae. albopictus U6 (AALF029726) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLib6.4-Aaeg_763

    Plasmid

    #176658

    Purpose

    Expression of sgRNA under Ae. Aegypti U6 (AAEL017763) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. 5xtetO (tet3) Gene Desert

    Plasmid

    #131339

    Purpose

    gRNA to insert 5xTet operator sequence into a gene desert on chromosome 5.

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    Desert-gRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pUPD_OsU3 (GB1184)

    Plasmid

    #68209

    Purpose

    Provides the O. sativa U3 RNA polIII promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    OsU3 promoter
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSG5-Myr-FLAG-p110β-DM

    Plasmid

    #55719

    Purpose

    eukaryotic expression of myristoylation tag (Myr-FLAG) p110b RBD double mutant S205D, K224A

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    p110 beta
    

    Tags

    myristoylation tag (Myr-FLAG)

    Expression

    Mammalian

    Mutation

    S205D, K224A

    Promoter

    T7

    Available Since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

38. JN402: pMVP (R4-R3) Firefly Luciferase

    Plasmid

    #121741

    Purpose

    pMVP R4-R3 entry plasmid, contains Firefly Luciferase for 3- or 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Firefly Luciferase
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCLC3sFlag

    Plasmid

    #52422

    Purpose

    mammalian expression of flag tagged rat CLC3 short transcript

    Depositor

    Steven Weinman

    Article

    Shimada et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    CLC3 (Clcn3 Rat)
    

    Tags

    flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pPAtUbq10 (GB0223)

    Plasmid

    #68174

    Purpose

    Provides the Arabidopsis thaliana polyubiquitin 10 promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtUbq10 promoter
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. KW701: pMVP (L5-L4) AID

    Plasmid

    #121723

    Purpose

    pMVP L5-L4 entry plasmid, contains AID for 4-component MultiSite Gateway Pro assembly. Allows fusion of minimal N-term Auxin Induced Degron (AID) domain (activated by auxin) to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    AID (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA-RELΔ424-490

    Plasmid

    #27267

    Depositor

    Thomas Gilmore

    Article

    Starczynowski et al Oncogene. 2007 Apr 26. 26(19):2685-94.

    Insert

    REL (REL Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of transactivation domain I (aa 425-490)

    Available Since

    Feb. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

43. II901: pMVP (L5-L4) FKBP DD

    Plasmid

    #121720

    Purpose

    pMVP L5-L4 entry plasmid, contains FKBP DD for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term FKBP degron domain (stabilized by SHLD1) to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    FKBP Degron Domain (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. UAS-Xbp1-GFP

    Plasmid

    #80627

    Purpose

    expression of Xbp1-GFP (RA isoform) in Drosophila

    Depositor

    Pedro Domingos, Herman Steller

    Article

    Ryoo et al EMBO J. 2007 Jan 10;26(1):242-52. Epub 2

    Insert

    Xbp1 (Xbp1 Fly)
    

    Tags

    EGFP

    Expression

    Insect

    Mutation

    RA isoform

    Promoter

    UAS

    Available Since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

45. pCellFree_G03 CSNK2A1

    Plasmid

    #67085

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CSNK2A1 (CSNK2A1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

46. pGSTag Flag Jnk3a1(2nd methione)

    Plasmid

    #15746

    Depositor

    Roger Davis

    Article

    Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

    Insert

    Jnk3a1-2nd M (MAPK10 Human)
    

    Expression

    Bacterial

    Available Since

    March 6, 2008

    Availability

    Academic Institutions and Nonprofits only

47. pEM01

    Plasmid

    #135414

    Purpose

    Expression of Hygromycin resistance from CMV promoter

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    CMVpr hph ADH1t
    

    Expression

    Bacterial and Yeast

    Promoter

    CMVpr

    Available Since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pET29b-AviTag-eGFP-dspB(E184Q)-6xHis

    Plasmid

    #175784

    Purpose

    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

    Insert

    Dispersin B
    

    Tags

    AviTag, Hexahistidine tag, and eGFP

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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49. C. difficile 16S toehold switch sensor

    Plasmid

    #110704

    Purpose

    Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

    Depositor

    James Collins

    Article

    Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

    Insert

    C. difficile 16S toehold switch sensor
    

    Use

    Synthetic Biology

    Promoter

    T7

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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50. JARID2-KO-gRNA-1

    Plasmid

    #131328

    Purpose

    gRNA to knockout JARID2. Use with JARID2-KO-gRNA-2

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    JARID2-KO-gRNA-1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only