159,258 results

1. pCMV-Bxb1

   Plasmid

   #182142

   Purpose

   Expression of human codon optimized Bxb1

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   human codon optimized Bxb1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-EglN1-pcDNA3

   Plasmid

   #18963

   Depositor

   William Kaelin

   Article

   Ivan et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

   Insert

   Egg laying Nine - 1 (EGLN1 Human)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   wt cDNA

   Promoter

   Available since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. mito-V5-APEX2

   Plasmid

   #72480

   Purpose

   Expresses APEX2 in mitochondria

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   APEX2
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-tMCK-ER-TurboID

   Plasmid

   #149412

   Purpose

   Muscle-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

   Depositor

   Jonathan Long

   Article

   Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

   Insert

   ER-TurboID
   

   Use

   AAV

   Tags

   HA and V5

   Expression

   Mutation

   Promoter

   tMCK

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. lenti-EF1a-dCas9-VPR-Puro

   Plasmid

   #99373

   Purpose

   3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Insert

   (Sp)dCas9-VPR-P2A-Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-TetOn-mCherry-OAZ1_FS-YFP

   Plasmid

   #232356

   Purpose

   Mammalian expression of dox-inducible polyamine sensor (polyamine levels = YFP/Cherry)

   Depositor

   Ankur Jain

   Article

   Sharma et al bioRxiv [Preprint]. 2024 Nov 17:2024.08.

   Insert

   OAZ1 derived polyamine sensing module (OAZ1 Human)
   

   Use

   Lentiviral

   Tags

   eYFP and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   March 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWPXL

   Plasmid

   #12257

   Purpose

   2nd generation lentiviral transfer plasmid

   Depositor

   Didier Trono

   Type

   Empty backbone

   Use

   Cre/Lox and Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-C1-PLCdelta-PH

   Plasmid

   #21179

   Purpose

   encodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipids

   Depositor

   Tobias Meyer

   Article

   Stauffer et al Curr Biol. 1998 Mar 12. 8(6):343-6.

   Insert

   PLCdelta (PLCD1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   PH domain of human PLC delta, amino acids Met1–Il…

   Promoter

   Available since

   July 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. EZ-Tet-pLKO-Blast

   Plasmid

   #85973

   Purpose

   Tet/Dox inducible shRNA lentivirus with blasticidin selection

   Depositor

   Cindy Miranti

   Article

   Frank et al BMC Biotechnol. 2017 Feb 28;17(1):24. do

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   H1 / TetO

   Available since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP SNAP23

    Plasmid

    #101914

    Purpose

    Expresses SNAP23 in mammalian cells

    Depositor

    Thierry Galli

    Article

    Kuster et al J Biol Chem. 2015 Nov 20;290(47):28056-6

    Insert

    SNAP 23 (SNAP23 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSEVA451-Cpf1

    Plasmid

    #192362

    Purpose

    CRISPR-based vector for genome editing

    Depositor

    Juana María Navarro Llorens

    Article

    Baldanta et al Microb Cell Fact. 2022 May 28;21(1):103.

    Insert

    Cas12a, gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAVS1-MAFB-IRF8-CEBPA

    Plasmid

    #190720

    Purpose

    For microglia differentiation from iPSC

    Depositor

    Li Gan

    Article

    Drager et al Nat Neurosci. 2022 Aug 11. pii: 10.1038/

    Insert

    MAFB-IRF8-CEBPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. SpyCatcher003-mi3

    Plasmid

    #159995

    Purpose

    Expresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccination

    Depositor

    Mark Howarth

    Article

    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

    Insert

    SpyCatcher003-mi3
    

    Use

    Tags

    C-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. 1227 pcDNA3 Myr HA Akt2

    Plasmid

    #9016

    Depositor

    William Sellers

    Insert

    Akt2 (AKT2 Human)
    

    Use

    Tags

    HA and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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15. Plenti-CMV-MCS-RFP-SV-puro

    Plasmid

    #109377

    Purpose

    This plasmid expresses dsRed2 and puromycin resistance

    Depositor

    Jonathan Garlick, Behzad Gerami-Naini

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    dsRed2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFR WT

    Plasmid

    #11011

    Purpose

    Retroviral construct for expressing human EGFR in human cells

    Depositor

    Matthew Meyerson

    Article

    Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. mNeonGreen-mTurquoise2

    Plasmid

    #98886

    Purpose

    Produces a fusion between mTurquoise2 and mNeonGreen. It can be used as a positive control for FRET

    Depositor

    Dorus Gadella

    Article

    Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

    Insert

    mNeonGreen-mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid

    #138147

    Purpose

    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    CasRx
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLCAR-CD19-CD3z

    Plasmid

    #135993

    Purpose

    Modular CD3z alone CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Scott McComb

    Article

    Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

    Insert

    FMC63-3z-P2A-EGFP (CD247, CD28 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3 APEX2-NES

    Plasmid

    #49386

    Purpose

    Expresses APEX2 in the cytosol of mammalian cells

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    Flag - APEX2 - NES
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K14D, W41F, E112K, A134P on soybean APX; improved…

    Promoter

    CMV

    Available since

    Nov. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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