We narrowed to 7,923 results for: AMPH

1. Cas12c-sgRNA_BbsI_CAM

   Plasmid

   #183221

   Purpose

   Cas12c sgRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   Cas12c single guide RNA containing BbsI sites in spacer sequence
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSB_init_Sb92

   Plasmid

   #159424

   Purpose

   Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb92

   Depositor

   Dianfan Li

   Article

   Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

   Insert

   Sb92
   

   Tags

   c-Myc and poly-His

   Expression

   Bacterial

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST1K18ms

   Plasmid

   #72646

   Purpose

   Gateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringae

   Depositor

   Brian Kvitko

   Article

   Mijatovic et al Phytopathology. 2021 Dec;111(12):2176-21

   Type

   Empty backbone

   Use

   Sucrose counter-selection allelic exchange vector

   Available Since

   March 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pKS1

   Plasmid

   #24636

   Depositor

   Jay Keasling

   Article

   Steen et al Nature. 2010 Jan 28;463(7280):559-62.

   Insert

   'tesA
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 14, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pSB_init_Sb68

   Plasmid

   #159423

   Purpose

   Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb68

   Depositor

   Dianfan Li

   Article

   Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

   Insert

   Sb68
   

   Tags

   c-Myc and poly-His

   Expression

   Bacterial

   Available Since

   Jan. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDRFLIP42

   Plasmid

   #65518

   Purpose

   FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

   Depositor

   Wolf Frommer

   Article

   Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

   Type

   Empty backbone

   Tags

   6His-Cit-attR1 and attR2-mCer-cMyc

   Expression

   Yeast

   Promoter

   pPMA1

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDRFLIP35

   Plasmid

   #65513

   Purpose

   FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

   Depositor

   Wolf Frommer

   Article

   Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

   Type

   Empty backbone

   Tags

   6His-AFPt9-attR1 and attR2-TFPt9-cMyc

   Expression

   Yeast

   Promoter

   pPMA1

   Available Since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDRFLIP32

   Plasmid

   #65511

   Purpose

   FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

   Depositor

   Wolf Frommer

   Article

   Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

   Type

   Empty backbone

   Tags

   6His-AFPt9-attR1 and attR2-t7CFPt9-cMyc

   Expression

   Yeast

   Promoter

   pPMA1

   Available Since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJC225

   Plasmid

   #176883

   Purpose

   MoClo Level1 plasmid, allows transition into Cermak, et al., 2017 GG cloning system

   Depositor

   Daniel Voytas

   Article

   Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Cloning

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pACYC_HA-ZmSAE1_ZmSAE2-FLAG

    Plasmid

    #91944

    Purpose

    Bacterial expression of maize SAE1 and SAE2

    Depositor

    Richard Vierstra

    Article

    Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

    Inserts

    HA-ZmSAE1
    ZmSAE2-FLAG
    

    Tags

    FLAG and HA

    Expression

    Bacterial

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPTK003-2-pENO1

    Plasmid

    #84962

    Purpose

    Type 2, pENO1, Enolase 1 promoter

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    Enolase 1 promoter
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Mutation

    BsaI site removed (2350 g to c) and BsmBI site re…

    Promoter

    Non

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR-P5P4

    Plasmid

    #141064

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFA-GFP

    Plasmid

    #133009

    Purpose

    pFA carrying a sfGFP gene

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Insert

    sfGFP
    

    Tags

    6xhis

    Expression

    Bacterial

    Promoter

    tetR

    Available Since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKS011

    Plasmid

    #65464

    Purpose

    pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) MBP (Maltose Binding Protein), Ntag, and mCherry

    Depositor

    Keith Tyo

    Article

    Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

    Insert

    MBP-Ntag-mCherry
    

    Tags

    FLAG and MBP

    Expression

    Bacterial

    Promoter

    pl-TetO

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. CTAG_mScarlet-I

    Plasmid

    #136118

    Purpose

    FP for C-term fusion with CDS12

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CTAG-mScarletI(noATG)
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pmMaple2-CAM

    Plasmid

    #101149

    Purpose

    Fluorescent protein for superresolution imaging

    Depositor

    Xiaowei Zhuang

    Article

    Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

    Insert

    E. coli codon optimized mMaple2
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCJH065_proD_Cas12c_DualRNA-GFP+HDVrz_CAM

    Plasmid

    #183077

    Purpose

    Plasmid expressing Cas12c tracrRNA and GFP-targeting crRNA separately, with the crRNA ending with a HDV ribozyme sequence.

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c tracrRNA and crRNA ending with a HDV ribozyme
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. LoriT

    Plasmid

    #163691

    Purpose

    Modified L4440 containing now CamR and oriT for conjugation

    Depositor

    Baris Tursun

    Article

    Kazmierczak et al Nucleic Acids Res. 2021 Feb 26;49(4):e22

    Insert

    oriT
    

    Expression

    Bacterial

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pReporter_7

    Plasmid

    #60567

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_7
    

    Expression

    Bacterial

    Promoter

    BBa_J23119

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSBDEST.N

    Plasmid

    #79468

    Purpose

    Promoterless, Sleeping Beauty-compatible Gateway destination vector, Neomycin

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Type

    Empty backbone

    Use

    Promoterless gateway destination vector

    Promoter

    none

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pGL4.23-SCP1-ccdB

    Plasmid

    #131628

    Purpose

    Modified STARR-seq plasmid to measure enhancer activity (SCP1 promoter; ccdb gene at cloning site)

    Depositor

    Kevin White

    Article

    Liu et al Genome Biol. 2017 Nov 20;18(1):219. doi:

    Type

    Empty backbone

    Use

    Luciferase

    Expression

    Bacterial and Mammalian

    Promoter

    SCP1

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. MTK4a_020

    Plasmid

    #123833

    Purpose

    Encodes the d4 PEST tag as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    d4PEST tag
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pJB089

    Plasmid

    #98914

    Purpose

    Express Dronpa tagged ZapA and pAmCherry1 tagged FtsZ in E coli

    Depositor

    Jie Xiao

    Article

    Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

    Inserts

    FtsZ
    ZapA
    

    Tags

    Dronpa and pAmCherry1

    Expression

    Bacterial

    Promoter

    T5-lac

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. pTU2-a-RFP

    Plasmid

    #72958

    Purpose

    Level 2 Destination vector - Position A - Assembly of 2 TU's from Level 1-AB

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 2 - a site
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

25. pMM0530

    Plasmid

    #128987

    Purpose

    Part plasmid for Zif268DBD-CRY2PHR, Type 3 (coding sequence) part

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    SV40NLS-ZiF268DBD-CRY2PHR
    

    Use

    Synthetic Biology

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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26. MTK3a_007

    Plasmid

    #123760

    Purpose

    Encodes Peroxisome Targeting Sequence as a Type 3a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PTS1
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAC-EsaR-V220A

    Plasmid

    #47645

    Purpose

    pAC-EsaR with a c659t mutation in esaR

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

    Insert

    esaR-V220A
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Changed Valine 220 to Alanine

    Promoter

    Plac

    Available Since

    Sept. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

28. pGBW-m4046865

    Plasmid

    #153930

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 3'-to-5' exonuclease

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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29. pSBDEST.H

    Plasmid

    #79464

    Purpose

    Promoterless, Sleeping Beauty-compatible Gateway destination vector, Hygromycin selection

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Type

    Empty backbone

    Use

    Promoterless gateway destination vector

    Promoter

    none

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 3918-37

    Plasmid

    #176495

    Purpose

    pTRE-EGFP-T2A-mCherry-polyA

    Depositor

    Albert La Spada

    Article

    Hsu et al Nat Commun. 2018 Mar 5;9(1):942. doi: 10

    Insert

    Tetracyline Responsive Element driving EGFP-T2A-mCherry
    

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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