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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 12,555 results for: LIC

Showing: 6601 - 6650 of 12555 results

  6. pET PI-SceI C-His

    Plasmid
    #195610
    Purpose
    Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter with a carboxyl-terminal histidine tag
    Depositor
    Frederick Gimble
    Article
    He et al J Biol Chem. 1998 Feb 20;273(8):4607-15.
    Insert
    PI-SceI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    T825C, C900G, A901C, A903C, T1131G, T1137C, T1227…
    Promoter
    T7
    Available since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    195610-plasmid-map-sequence-id-383258

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  9. Linked mRuby3 VLP

    Plasmid
    #182430
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-GFP-mRuby3 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-GFP-mRuby3
    deltaVP1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182430-plasmid-map-sequence-id-360112

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  10. Coexpressed mRuby3 VLP

    Plasmid
    #182431
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-GFP (GAL10 promoter), and VP2C-mRuby3 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-GFP
    deltaVP1
    VP2C-mRuby3
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1, GAL10, and GAL7
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182431-plasmid-map-sequence-id-360113

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  16. pILGFP4D41

    Plasmid
    #185841
    Purpose
    Testing the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1-PCYC1+[Z268]>yEGFP>TPGK1-TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    185841-plasmid-map-sequence-id-369628

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  18. pILGFP8EFA

    Plasmid
    #185854
    Purpose
    Testing a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    DHFR P66L
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185854-plasmid-map-sequence-id-370766

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  21. pRSF-G1pCNPRS

    Plasmid
    #174719
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-5-pyridyl)alanine (pCNP), into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Article
    Abdelkader et al Angew Chem Int Ed Engl. 2022 Jan 31. doi
    Insert
    tRNA synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    174719-plasmid-map-sequence-id-346622

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  36. pMC6-spacer

    Plasmid
    #176178
    Purpose
    yeast MoClo level-0 part plasmid type 6 containing non-coding DNA spacer for construction of MoClo plasmids without yeast marker
    Depositor
    Florian David
    Article
    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-
    Insert
    41 bp non-coding DNA spacer from pYTK048
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    176178-plasmid-map-sequence-id-353641

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  38. F9F17F-GFP

    Plasmid
    #163679
    Purpose
    Expresses a TFR mutant with truncated cytosolic tail and TMD in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    transferrin receptor mutant (TFRC Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This chimera contains 9 amino acids from the cyto…
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163679-plasmid-map-sequence-id-322819

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  39. pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis

    Plasmid
    #176575
    Purpose
    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.
    Depositor
    Mark Nitz
    Article
    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:
    Insert
    Dispersin B
    Use
    Tags
    Avitag, Hexahistidine tag, and mEGFP
    Expression
    Bacterial
    Mutation
    E184Q W330Y
    Promoter
    T7
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    176575-plasmid-map-sequence-id-348273

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Showing: 6601 - 6650 of 12555 results