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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 6,963 results for: RAP

Showing: 6601 - 6650 of 6963 results

  3. MZB139

    Plasmid
    #217838
    Purpose
    Empty 10xHis-SUMO (Smt3) vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Article
    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    SMT3 (SMT3 Budding Yeast)
    Use
    Tags
    10x His and SUMO (SMT3)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    217838-plasmid-map-sequence-id-432993

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  4. MZB164

    Plasmid
    #217842
    Purpose
    10xHis-SUMO (Smt3) fused, HRV-3C cleavable, Halotag for rapid cloning/DNA assembly of pulldown targets via SwaI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Article
    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    HaloTag
    Use
    Tags
    10x His, HaloTag, and SUMO (SMT3)
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    217842-plasmid-map-sequence-id-433000

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  8. CD44I-c011

    Plasmid
    #175449
    Purpose
    Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N178. Used for crystallography.
    Depositor
    Opher Gileadi
    Insert
    CD44 (CD44 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175449-plasmid-map-sequence-id-353150

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  15. pCLASP_mScarlet_CLASP_RGS2membrane

    Plasmid
    #133086
    Purpose
    Plasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to bl
    Depositor
    Hana El-Samad
    Article
    Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2
    Insert
    mScarlet-CLASP
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    133086-plasmid-map-sequence-id-296450

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  19. pLentiCRISPRv2-sgAAVS1-HepmTurquoise2

    Plasmid
    #192828
    Purpose
    Expresses sgRNA targeting AAVS1 (non-targeting control sgRNA for mouse) and mTurquoise2 from hepatocyte-specific promoter
    Depositor
    Kristin Knouse
    Article
    Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do
    Insert
    mTurquoise2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 for sgRNA and hepatocyte-specific promoter (HS…
    Available since
    Nov. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    192828-plasmid-map-sequence-id-378004

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  28. p46Cpf1-OP2

    Plasmid
    #98592
    Purpose
    Produces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.
    Depositor
    Qiong Wu
    Article
    Ao et al Front Microbiol. 2018 Oct 9;9:2307. doi:
    Insert
    FnCpf1
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Codon optimized for E. coli
    Promoter
    araBAD
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    98592-plasmid-map-sequence-id-201329

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  31. pHPHS232

    Plasmid
    #228356
    Purpose
    Landing pad with Bxb1 attP_wildtype site, Dox-inducible BFP-iCasp9-Blasticidin, CMV-driven rtTA3, T2A-neomycin for HDR into AAVS1 locus
    Depositor
    Arvind Rasi Subramaniam
    Article
    Nugent et al bioRxiv [Preprint]. 2024 Jul 26:2024.07.
    Inserts
    mTagBFP2
    Reverse tetracycline transactivator 3
    neo
    Use
    Tags
    Blasticidin S deaminase and iCasp9
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3GV and gene trap
    Available since
    Jan. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228356-plasmid-map-sequence-id-455149

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  36. FRB_LD 0_CS G_tTA-BFP

    Plasmid
    #58876
    Purpose
    MESA target chain with Rapamycin FRB ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA target chain with FRB ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    Use
    AAV
    Tags
    EBFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    58876-plasmid-map-sequence-id-99612

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  43. FRB_nSuMMVp

    Plasmid
    #118970
    Purpose
    Expresses N-terminus of sunflower mild mosaic virus protease (SuMMVp) fused to the FKBP-rapamycin binding (FRB) domain
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split nSuMMVp in fusion with FRB
    Use
    Tags
    Myc tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    118970-plasmid-map-sequence-id-231812

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Showing: 6601 - 6650 of 6963 results