We narrowed to 11,551 results for: NSI

1. pAS_FLAG-Mma PylRS

   Plasmid

   #154762

   Purpose

   plasmid with Mma PylRS for amber suppression in transient or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2 CA-LRP6-GFP

   Plasmid

   #29682

   Depositor

   Edward De Robertis

   Article

   Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

   Insert

   LDL-receptor related protein 6 (LRP6 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   extracellular region deleted

   Promoter

   Available Since

   July 7, 2011

   Availability

   Academic Institutions and Nonprofits only

3. MAC_C_PTH2

   Plasmid

   #111674

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   PTH2 (PTRH2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TRS6E1b-luc(-732/-721) WT

   Plasmid

   #45383

   Depositor

   Thomas Gelehrter

   Article

   Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

   Insert

   6 copies of hPAI-1 promoter -732/-721
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 20, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pDR-GFPuniv

   Plasmid

   #46085

   Purpose

   In vivo recombination assay fluorescent reporter

   Depositor

   Raymond Monnat

   Article

   Li et al Nucleic Acids Res. 2012 Mar;40(6):2587-9

   Insert

   DR-GFPuniv reporter
   

   Use

   Recombination reporter plasmid

   Tags

   Expression

   Mammalian

   Mutation

   E5G and K163R mutations in EGFP relative to wild-…

   Promoter

   Available Since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-CALPAIN-7-mCherry

   Plasmid

   #180652

   Purpose

   Lentiviral expression vector for CALPAIN-7. Used for generating cell lines. Has C-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-50.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CALPAIN-7
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   siRNA resistant to: GCACCCAUACCUUUACAUU

   Promoter

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHis-hMTMR1

   Plasmid

   #100723

   Purpose

   Expression of human MTMR1 PH-PTP domain

   Depositor

   Byung Il Lee

   Article

   Bong et al PLoS One. 2016 Mar 28;11(3):e0152611. do

   Insert

   MTMR1 (MTMR1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains amino acids 95-607

   Promoter

   T7

   Available Since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. TDP43 NOTAG2

   Plasmid

   #28207

   Depositor

   Zuoshang Xu

   Article

   Yang et al PLoS One. 2010 . 5(12):e15878.

   Insert

   Tar DNA binding protein (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   aa86-414 only

   Promoter

   Available Since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

9. BAS286 C terminal SF-GFP hygBR

   Plasmid

   #74081

   Purpose

   S.pombe recoded superfolder GFP in universal C-terminal tagging plasmid, hygB resistanc

   Depositor

   Bassem Al-Sady

   Article

   Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

   Insert

   super-folder GFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

10. GFP-OSH2-2xPH

    Plasmid

    #161987

    Purpose

    Mammalian expression vector, contains two tandem repeats of OSH2 PH domain (PI(4)P biosensor). Fused to eGFP

    Depositor

    Tamas Balla, David Bryant

    Article

    Balla et al Mol Biol Cell. 2008 Feb;19(2):711-21. do

    Insert

    OSH2 (OSH2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRK5-mFzd2

    Plasmid

    #42254

    Depositor

    Chris Garcia, Jeremy Nathans

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 2 (Fzd2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPBpuro-miRFP670-HRasCT

    Plasmid

    #176030

    Purpose

    Encoding membrane-targeted miRFP670

    Depositor

    Michiyuki Matsuda

    Article

    Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

    Insert

    Membrane-targeted miRFP670
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTY07

    Plasmid

    #83285

    Purpose

    human CENP-A N-terminally labeled with LSSmOrange

    Depositor

    Daniel Needleman

    Article

    Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

    Insert

    centromere protein A (CENPA Human)
    

    Use

    Retroviral

    Tags

    LSSmOrange

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-RAB-EKARev(T/A)

    Plasmid

    #118475

    Purpose

    Negative-control mutant for RAB-EKARev biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    RAB-EKARev(T/A)
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV7.1_3xFLAG-WWC3

    Plasmid

    #172996

    Purpose

    constitutive expression of FLAG-tagged WWC3

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-WWC3 (WWC3 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-UAS-mIFNγ

    Plasmid

    #230004

    Purpose

    Gal4-dependent expression of secreted mouse IFNγ

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    UAS-mIFNγ (Ifng Synthetic)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UAS

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-ExRai-AktAR(T/A)

    Plasmid

    #118463

    Purpose

    Negative-control mutant for ExRai-AktAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AktAR(T/A)
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRK5-mFzd5-1D4

    Plasmid

    #42267

    Depositor

    Chris Garcia, Jeremy Nathans

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 5 (Fzd5 Mouse)
    

    Use

    Tags

    1D4

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYFAC-CH2

    Plasmid

    #168978

    Purpose

    AMA1 based expression in filamentous fungi pYFAC-pyrG carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA

    Depositor

    Yit Heng Chooi

    Article

    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

    Type

    Empty backbone

    Use

    Synthetic Biology; Heterologous expression in asp…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGB 35S:DsRed:Tnos (GB0361)

    Plasmid

    #68220

    Purpose

    Transcriptional unit for DsRed plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    DsRed
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. pYSD099

    Plasmid

    #212923

    Purpose

    A MoClo Yeast Toolkit LEU2, CEN/ARS empty vector (GFP dropout). Contains ConL1, part234 GFP dropout, ConR2, LEU2, CEN6/ARS4, AmpR-ColE1.

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPerimCh

    Plasmid

    #227013

    Purpose

    Expresses periplasmic mCherry

    Depositor

    Suzan Rooijakkers

    Article

    Heesterbeek et al Sci Rep. 2019 Feb 28;9(1):3074. doi: 10.

    Insert

    PelB-mCherry, arabinose inducible cytosolic GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rpsM and pBAD

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSNAP-CLC-EYFP

    Plasmid

    #38011

    Depositor

    Xiaowei Zhuang

    Article

    Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

    Insert

    Clathrin, light polypeptide (LCa) (Clta Mouse)
    

    Use

    Tags

    EYFP and SNAP26m

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pYDE007

    Plasmid

    #194152

    Purpose

    sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, CarbR. Contains mrfp nontargeting control guide sequence.

    Depositor

    Edward Geisinger

    Article

    Bai et al J Bacteriol. 2021 May 20;203(12):e005652

    Insert

    sgRNA with mrfp control guide sequence
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti-CaMKIIa-Archer1-EGFP

    Plasmid

    #60423

    Purpose

    Lentiviral vector with CaMKIIa promoter and upstream CMV promoter expressing Archer1-EGFP fusion. Archer1's red fluorescence monitors changes in membrane voltage.

    Depositor

    Viviana Gradinaru

    Article

    Flytzanis et al Nat Commun. 2014 Sep 15;5:4894. doi: 10.

    Insert

    Archer1-EGFP
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pYFAC-CH4

    Plasmid

    #168980

    Purpose

    AMA1 based expression in filamentous fungi.pYFAC-pyroA carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA

    Depositor

    Yit Heng Chooi

    Article

    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

    Type

    Empty backbone

    Use

    Synthetic Biology; Heterologous expression in asp…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCSIIbsr-4082NES(ForAMPK)

    Plasmid

    #170310

    Purpose

    The lentiviral vector for AMPK FRET-biosensor

    Depositor

    Michiyuki Matsuda

    Article

    Konagaya et al Cell Rep. 2017 Nov 28;21(9):2628-2638. d

    Insert

    4082NES (FRET biosensor for AMPK activity)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3-Nuc-ExRai-AMPKAR

    Plasmid

    #192450

    Purpose

    ExRai-AMPKAR targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR-NLS
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. N1-mSin1.2-GFP

    Plasmid

    #72908

    Purpose

    GFP-tagged mSin1 (isoform 2)

    Depositor

    Ivan Yudushkin

    Article

    Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

    Insert

    Target of rapamycin complex 2 subunit MAPKAP1, isoform 2 (MAPKAP1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGEX4T-HEPN

    Plasmid

    #75296

    Purpose

    Expresses Sacsin HEPN domain (4441-4579) in fusion with GST (bacterial)

    Depositor

    Kalle Gehring

    Article

    Kozlov et al J Biol Chem. 2011 Jun 10;286(23):20407-1

    Insert

    Human Sacsin 4441-4579
    

    Use

    Tags

    GST-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAB076i5

    Plasmid

    #79199

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1+2+3) gIII, luxAB; Ppro1 434cI-SH2ABL1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (OR1+2+3); Ppro1

    Available Since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHAGE-UBC-TagRFP-T-ps

    Plasmid

    #178656

    Purpose

    Expression of palmitoylated TagRFP-T for live imaging of plasma membrane (protrusions, tunneing nanotubes), in mammalian cells

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al Proc Natl Acad Sci U S A. 2017 Nov 14;11

    Insert

    TagRFP-T with palmitoylation signal at N-terminus
    

    Use

    Lentiviral

    Tags

    Palmitoylation signal

    Expression

    Mutation

    Promoter

    Ubc

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA FLAG TORC3

    Plasmid

    #22976

    Depositor

    Marc Montminy

    Article

    Screaton et al Cell. 2004 Oct 1. 119(1):61-74.

    Insert

    TORC3 (CRTC3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

34. pHAGE-IRES-puro-NLS-NLS-dPba3Cas13b-3xGFP-NLS

    Plasmid

    #191369

    Purpose

    Overexpression of NLS-NLS-dPba3Cas13b-3xGFP-NLS in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

    Insert

    NLS-NLS-dPba3Cas13b-3xGFP-NLS
    

    Use

    Tags

    3xGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAB078d8

    Plasmid

    #79206

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1+2) luxAB; Ppro1 434cI-SH2ABL1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (OR1+2); Ppro1

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLicC-MBP-APETx2

    Plasmid

    #72668

    Purpose

    Periplasmic expression of APETx2 in E.coli

    Depositor

    Glenn King

    Article

    Anangi et al Mar Drugs. 2012 Jul;10(7):1605-18. doi:

    Insert

    APETx2
    

    Use

    Tags

    His tag, MBP, MalE signal sequence for periplasmi…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDV02_linear_entry_TagBFP-2A-MCS-2A-mNeonGreen

    Plasmid

    #200401

    Purpose

    Entry vector for open-loop ADAR-based RNA sensors. Expresses TagBFP constitutively. Expresses mNeonGreen upon target detection

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    Open-loop DART VADAR sensor entry cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pYFP-NGAT

    Plasmid

    #11383

    Depositor

    Joel Swanson

    Article

    Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

    Insert

    golgi associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pUWR-Cad-Venus

    Plasmid

    #58328

    Purpose

    Destination vector for inserting ubi-Cad-Venus to Drosophila melanogaster genome

    Depositor

    Denise Montell

    Article

    Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

    Insert

    Cad-Venus
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    poly-ubiquitin

    Available Since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

40. pLenti CMVie-IRES-BlastR alt MCS

    Plasmid

    #120862

    Purpose

    3rd generation lentiviral expression under a CMVie promoter with Blasticidin selection

    Depositor

    Ghassan Mouneimne

    Article

    Puleo et al J Cell Biol. 2019 Oct 8. pii: jcb.201902

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV immediate early

    Available Since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge