We narrowed to 23,108 results for: crispr

1. pYPQ134B

   Plasmid

   #179216

   Purpose

   Golden Gate entry vector; 4th gRNA under AtU3 promoter

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   AtU3

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCfB4906

   Plasmid

   #106150

   Purpose

   EasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying hygromycin resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. qTAG-N-Zeo-moxGFP

   Plasmid

   #207699

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-moxGFP cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pJJB302

   Plasmid

   #107694

   Purpose

   OsActin Promoter-LwaCas13a-HSP Terminator

   Depositor

   Daniel Voytas

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   Cas13a
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   OsActin

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pYPQ134A

   Plasmid

   #69276

   Purpose

   Golden Gate entry vector; 4th gRNA under AtU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. qTAG-N-Puro-mNeon

   Plasmid

   #207687

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mNeon cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. qTAG-N-Puro-3xFLAG

   Plasmid

   #207695

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-3xFLAG cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. p221z-CAS9p-TagRFP-t35s

   Plasmid

   #118386

   Purpose

   Entry clone containing CAS9p-TagRFP and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

   Insert

   CAS9p-TagRFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSF280

   Plasmid

   #167675

   Purpose

   Intermediate vector for six gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. qTAG-C-mNeon-Puro

    Plasmid

    #207720

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLBH559_Tet-HisCas14a1Locus

    Plasmid

    #112502

    Purpose

    Expresses 10xHis-Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14a1
    

    Use

    CRISPR

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CL7-LbCas12a

    Plasmid

    #164658

    Purpose

    Bacterial expression of LbCas12a using CL7/Im7

    Depositor

    Piyush Jain

    Article

    Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

    Insert

    LbCas12a
    

    Use

    Tags

    Thioredoxin, CL7, SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. qTAG-N-Blast-dTAG

    Plasmid

    #207683

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-dTAG cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pAAV-SCP1-dSa-VPR mini.

    Plasmid

    #99685

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. px458_2A_GFP_sgRNA_TIA1

    Plasmid

    #106097

    Purpose

    CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting TIA1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    gRNA TIA1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. qTAG-N-Blast-3xFLAG

    Plasmid

    #207684

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xFLAG cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pYPQ132-tRNA

    Plasmid

    #179211

    Purpose

    Golden Gate entry vector; 2nd gRNA with tRNA

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    no promoter

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. qTAG-N-Blast-miniTurbo

    Plasmid

    #207681

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-miniTurbo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. p2CT-His-MBP-Lba_Cas13a_WT

    Plasmid

    #91867

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    LbaCas13a (LbaC2c2) WT
    

    Use

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. qTAG-C-sTagRFP-Puro

    Plasmid

    #207723

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. Level 1 P4 TaU6 guide acceptor

    Plasmid

    #165600

    Purpose

    GoldenGate (MoClo) Level 1 Position 4 TaU6 guide acceptor plasmid

    Depositor

    John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

    Article

    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

    Insert

    TaU6 promoter::LacZ::sgRNA scaffold
    

    Use

    CRISPR and Synthetic Biology; Sgrna acceptor with…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Level 1 P3 TaU6 guide acceptor

    Plasmid

    #165599

    Purpose

    GoldenGate (MoClo) Level 1 Position 3 TaU6 guide acceptor plasmid

    Depositor

    John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

    Article

    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

    Insert

    TaU6 promoter::LacZ::sgRNA scaffold
    

    Use

    CRISPR and Synthetic Biology; Sgrna acceptor with…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Spacer Switch Plasmid

    Plasmid

    #215346

    Purpose

    Facilitates the change in spacer sequence to Neisseria gonorrhoeae encoding the Nla CRISPRi system.

    Depositor

    Hank Seifert

    Article

    Geslewitz et al mBio. 2023 Dec 21:e0302523. doi: 10.1128

    Insert

    OpaD spacer; Chloramphenicol Resistance Cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pWalium20-10XUAS-3XFLAG-dCas9-VPR

    Plasmid

    #78897

    Purpose

    Expresses 3XFLAG-dCas9-VPR (human codon) under 10XUAS control, for in vivo CRISPRa

    Depositor

    Norbert Perrimon

    Article

    Lin et al Genetics. 2015 Oct;201(2):433-42. doi: 1

    Insert

    dCas9-VPR
    

    Use

    Tags

    3xFLAG

    Expression

    Insect

    Mutation

    Promoter

    10XUAS

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. qTAG-C-3xHA-Blast

    Plasmid

    #207718

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. qTAG-N-Blast-V5

    Plasmid

    #207686

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-V5 cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. qTAG-N-Puro-miniTurbo

    Plasmid

    #207692

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-miniTurbo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pLBH545_Tet-Cas14a1_Locus

    Plasmid

    #112501

    Purpose

    Expresses Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14a1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. qTAG-N-Zeo-3xFLAG

    Plasmid

    #207706

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xFLAG cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. qTAG-N-Blast-3xHA

    Plasmid

    #207685

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xHA cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pSF279

    Plasmid

    #167674

    Purpose

    Intermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMS79 (eft-3p::Cas9 + sgRNA)

    Plasmid

    #154839

    Purpose

    Cas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGG

    Depositor

    Patrick Phillips

    Article

    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. qTAG-C-mNeon-Blast

    Plasmid

    #207709

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Brp-T2A-QF2w-Hsp70-3XP3-dsRed

    Plasmid

    #141094

    Purpose

    Template plasmid for inserting QF2w into the Brp gene of Aedes aegypti with CRISPR/Cas9, to generate a pan-neuronal driver line.

    Depositor

    Carolyn McBride

    Article

    Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

    Insert

    bruchpilot
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    coding sequence downstream of CRISPR cut site, sy…

    Promoter

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. qTAG-N-Puro-ultraID

    Plasmid

    #207693

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-ultraID cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pYPQ133D

    Plasmid

    #69289

    Purpose

    Golden Gate entry vector; 1st gRNA under OsU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pN-PITCh-GFP

    Plasmid

    #127888

    Purpose

    Provides the repair template with PSMD2 microhomology on each end for PSMD2 N-ter GFP tagging

    Depositor

    Peter Kaiser

    Article

    Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

    Insert

    Puro-GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAC-U63-gRNA2.1

    Plasmid

    #170512

    Purpose

    gRNA cloning vector containing a U6:3 promoter and a gRNA2.1 scaffold.

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6:3

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. qTAG-C-miniTurbo-Blast

    Plasmid

    #207714

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. qTAG-C-moxGFP-Puro

    Plasmid

    #207721

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

41. pPPC009.106

    Plasmid

    #171142

    Purpose

    Expression of J106 scRNA on pBBR1-KmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Insert

    J106 scRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pME-Cas9

    Plasmid

    #64237

    Purpose

    expresses codon optimized Cas9 in zebrafish

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Insert

    codon optimized Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pMBP-AaCas12b

    Plasmid

    #113433

    Purpose

    Expresses Expresses 10xHis-MBP fused to AaCas12b

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    AaCas12b
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. qTAG-C-ultraID-Zeo

    Plasmid

    #207737

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

45. pZ149-pcDNA3-BhCas12b_v4

    Plasmid

    #122446

    Purpose

    Expresses BhCas12b_v4 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    CMV-NLS-BhCas12b_v4-NLS-3xHA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #112924

    Purpose

    piggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant marker

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
    

    Use

    Piggybac transposon vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pRU325

    Plasmid

    #167676

    Purpose

    Intermediate vector for eight gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. eGFP L202 gRNA

    Plasmid

    #119132

    Purpose

    gRNA that guides Cas9 and APOBEC complexes to L202 in eGFP.

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    eGFP L202 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    None

    Promoter

    U6

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. U6-(BbsI)sgRNA_CAG-Venus-bpA

    Plasmid

    #86985

    Purpose

    For cloning and expression of sgRNA together with Venus expression

    Depositor

    Ralf Kuehn

    Article

    Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

    Insert

    Venus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Venus GFP variant

    Promoter

    CAG

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. qTAG-N-Blast-moxGFP

    Plasmid

    #207677

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-moxGFP cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge