We narrowed to 29,718 results for: REP

1. pX458_miR-290-295KO-gRNA1

   Plasmid

   #172709

   Purpose

   Cas9 with 5' gRNA for paired CRISPR KO of miR-290-295 cluster

   Depositor

   Constance Ciaudo

   Article

   Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

   Insert

   miR-290-295
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX458_miR-290-295KO-gRNA2

   Plasmid

   #172710

   Purpose

   Cas9 with 3' gRNA for paired CRISPR KO of miR-290-295 cluster

   Depositor

   Constance Ciaudo

   Article

   Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

   Insert

   miR-290-295
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. sgLacZs

   Plasmid

   #171185

   Purpose

   Expresses sgRNA

   Depositor

   Han Kyoung Choe

   Article

   Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

   Insert

   hU6-sgLacZ1-hU6-sgLacZ2
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   U6, hSyn

   Available Since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. PXPR007 sgGFP-2

   Plasmid

   #74961

   Purpose

   Cas9 + sgGFP-2 with blasticidin selection

   Depositor

   William Hahn

   Article

   Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

   Insert

   GFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-Parkin-A92mKO2

   Plasmid

   #213548

   Purpose

   Transient mammalian expresssion of Parkin, internally tagged with mKO2

   Depositor

   Lani Wu, Steven Altschuler

   Article

   Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

   Insert

   Parkin (PRKN Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. hsp70:Stathmin1-mKate2

   Plasmid

   #105969

   Purpose

   heat shock inducible transgenesis, stathmin stmn1 orig. from addgene #31326

   Depositor

   Caren Norden

   Article

   Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

   Insert

   Stathmin1 (STMN1 Human)
   

   Use

   Tags

   mKate2

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. CNNM3-CBS-YPet

   Plasmid

   #149686

   Purpose

   For the FRET-based binding assay between human CNNM3 CBS domain and PRL2.

   Depositor

   Motoyuki Hattori

   Article

   Cai et al Sci Rep. 2020 Jul 30;10(1):12879. doi: 1

   Insert

   CNNM3-CBS-YPet (CNNM3 Human, Synthetic)
   

   Use

   Tags

   8 histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV-puro-TTF-1-HDD

   Plasmid

   #74991

   Purpose

   Retroviral vector holding human TTF-1 (NKX2-1) with the homeodomain deleted

   Depositor

   David Mu

   Article

   Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

   Insert

   TTF-1 HDD mutant (NKX2-1 Human)
   

   Use

   Retroviral

   Tags

   None

   Expression

   Mammalian

   Mutation

   HDD = homeodomain deleted

   Promoter

   Available Since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET32a-hAREG

   Plasmid

   #199229

   Purpose

   Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

   Depositor

   Nilson Zanchin

   Article

   Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

   Insert

   Amphiregulin preproprotein (AREG Human)
   

   Use

   Tags

   TrxA-6xHis-S-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Bxb1_jRCaMP1b

    Plasmid

    #183758

    Purpose

    Bxb1-specific donor plasmid for intergrating a transcription unit for expression of jRCaMP1b calcium reporter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    jRCaMP1b
    

    Use

    Tags

    NES

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-N1-2x

    Plasmid

    #172282

    Purpose

    Expressing EGFP mRNA fused with 2 tandem repeats of a 50-base sequence

    Depositor

    Antony K Chen

    Article

    Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

    Insert

    EGFP-N1-2x
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-N1-4x

    Plasmid

    #172283

    Purpose

    Expressing EGFP mRNA fused with 4 tandem repeats of a 50-base sequence

    Depositor

    Antony K Chen

    Article

    Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

    Insert

    EGFP-N1-4x
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti CMV TRE3G BCL2L11 G156E Neo

    Plasmid

    #140748

    Purpose

    Inducibly expresses G156E mutant BIM in mammalian cells

    Depositor

    Scott Dixon

    Article

    Inde et al Cell Rep. 2020 Jul 7;32(1):107845. doi:

    Insert

    BCL2L11 (BCL2L11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed Glycine 156 to Glutamate

    Promoter

    CMV TRE3G

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-CaMaKIIalpha-mKO2

    Plasmid

    #86156

    Purpose

    Lentiviral fluorescent reporter using the neuronal cell type-specific promoter of the CaM Kinase II alpha gene.

    Depositor

    James Ellis

    Article

    Rodrigues et al Cell Rep. 2016 Oct 11;17(3):720-734. doi

    Insert

    mKO2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. vsv-CenpB 1-166-Sgo1-GFP

    Plasmid

    #108495

    Purpose

    expression of vsv-CenpB-1-166-Sgo1-EGFP

    Depositor

    Susanne Lens

    Article

    Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

    Insert

    CenpB-Shugoshin 1 (SGO1 Human)
    

    Use

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    CenpB 1-166 fusion before Sgo1

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSF4 TetCMV intron 4xGCN4 Renilla FKBP STOP 24xMS2v5 SV40 CTE polyA

    Plasmid

    #104984

    Purpose

    Expresses ST-Renilla reporter in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

    Insert

    Renilla luciferase
    

    Use

    Tags

    24xMS2v5 stem loops in 3'UTR, 4xGCN4 repeats…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TetCMV

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CAGGS-Flex-Turbo/GFP

    Plasmid

    #197886

    Purpose

    Can be used to generate AAV virus that will express Turbo/GFP in the presence of Cre

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    Turbo/GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    CAG

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBlastR-MCS-QF-MiniWhite

    Plasmid

    #165906

    Purpose

    Blasticidin resistant QF driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable Empty Enhancer Driver
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NG1237 pCI-TPI-PTC160-4H

    Plasmid

    #65804

    Purpose

    NMD reporter mRNA

    Depositor

    Niels Gehring

    Article

    Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

    Insert

    TPI (TPI1 Human)
    

    Use

    Tags

    4H (4 copies of short beta-globin 3'UTR for …

    Expression

    Mammalian

    Mutation

    premature stop codon at 160

    Promoter

    CMV

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSN69

    Plasmid

    #100125

    Purpose

    expresses putative cMYC DBD with a HA tag

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCerulean-P2A-putative cMYC DBD with a HA tag (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Insert corresponds to the DBD of cMYC

    Promoter

    Available Since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMB1052:miniTol2-U6-(empty)SaCas9_gRNA-CAG-Cre-P2A-NeoR-WPRE

    Plasmid

    #168108

    Purpose

    Tol2 transposon vector of empty U6-S.aureus Cas9 gRNA cassette and CAG-driven Cre-NeoR

    Depositor

    Erich Jarvis

    Article

    Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 3xFN-Tel-TAL/pMXs-puro

    Plasmid

    #63590

    Purpose

    Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat. A retroviral expression vector with the puromycin resistance gene.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038

    Insert

    3xFVN-Tel-TAL
    

    Use

    Retroviral and TALEN

    Tags

    3xFLAG-tag, V5-tag, and nuclear localization sign…

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSHDY*in_PpetE:BCD2:mVenus

    Plasmid

    #133970

    Purpose

    Heterologous, copper-inducible expression of the reporter fluorophor mVenus in Synechocystis sp. PCC 6803. Used as backbone for cloning of GOI downstream of the PpetE:BCD2 expression module.

    Depositor

    Pia Lindberg

    Article

    Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.

    Insert

    mVenus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    inserted gene is codon optimized for Synechocyst…

    Promoter

    PpetE:BCD2

    Available Since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pT2-cryR;dll4CE2-P1Egfp

    Plasmid

    #90147

    Purpose

    contains dll4 enhancer 2 sequence, and dll4 specific gene promoter driving expression of eGFP

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    dll4E2P1Egfp (dll4 Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDNA3.1(-) + SLNCR1 _701-902

    Plasmid

    #86821

    Purpose

    mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    LINC00673 (LINC00673 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pG418R-MCS-QF-MiniWhite

    Plasmid

    #165902

    Purpose

    G418 resistant QF driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable Empty Enhancer Driver
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUCHR-CD5Flag2-P2A-smAID-mClover

    Plasmid

    #110657

    Purpose

    Lentiviral vector expressing cell surface Flag-tag and monomeric GFP N-terminaly fused with auxin inducible degron

    Depositor

    Dmitriy Mazurov

    Article

    Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

    Insert

    CD5Flag2-P2A-AID-mClover
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPN461

    Plasmid

    #137877

    Purpose

    PiggyBac vector for expression of 1x gRNA targeting POGZ for CRISPRi; mRFP-T2A-BlasticidinR

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    POGZ (POGZ Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1-MCS-BirA(R118G)-HA-MACROD1

    Plasmid

    #172581

    Purpose

    For transient expression of C-terminal BirA-tagged MACROD1

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    MACROD1 (MACROD1 Human)
    

    Use

    Tags

    BirA(R118G)-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAG 442TAA

    Plasmid

    #154779

    Purpose

    plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAG 277TAA, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    LaG17-SynNotch
    

    Use

    Tags

    LaG17

    Expression

    Mammalian

    Mutation

    204TAG 442TAA in LaG17-SynNotch, hybrid PylT with…

    Promoter

    EF1

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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