We narrowed to 16,109 results for: form

1. (F) pADH2 (G)_P2 (SBE113)

   Plasmid

   #187628

   Purpose

   promoter of the gene alcohol dehydrogenase 2 from R. toruloides fro the P2 position (F/G)

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   (F) pADH2 (G)
   

   Expression

   Bacterial

   Available Since

   Dec. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. (F) pXYL (G) P1 (SBE216)

   Plasmid

   #195017

   Purpose

   promoter of the gene xylose reductase from R. toruloides for the position P2 (F/G)

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   (F) pXYL (G)
   

   Expression

   Bacterial

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. (R) pXYL (D) P1 (SBE215)

   Plasmid

   #195016

   Purpose

   promoter of the gene xylose reductase from R. toruloides for the position P1 (R/D)

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   (R) pXYL (D)
   

   Expression

   Bacterial

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. (R)pADH2(D)_P1(SBE214)

   Plasmid

   #195015

   Purpose

   promoter of the gene alcohol dehydrogenase 2 from R. toruloides for the position P1

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   (B) pADH2 (R)
   

   Expression

   Bacterial

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. (J) crtYB(K) G3 (SBE117)

   Plasmid

   #194996

   Purpose

   Rhodotorula toruloides gene phytoene synthase/lycopene cyclase (crtYB, RHTO_04605) for G3

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   (J) crtYB(K)
   

   Expression

   Bacterial

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. (G) crtI (H) G2 (SBE116)

   Plasmid

   #194995

   Purpose

   Rhodotorula toruloides gene phytoene dehydrogenase (crtI, RHTO_04602) for G2

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   (G) crtI (H)
   

   Expression

   Bacterial

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. (D) crtE (E)_G1 (SBE115)

   Plasmid

   #194994

   Purpose

   Rhodotorula toruloides gene geranylgeranyl diphosphate synthase (crtE, RHTO_02504) for G1

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   (D) crtE (E)
   

   Expression

   Bacterial

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. (I) pGPD (J)_P3 (SBE111)

   Plasmid

   #194990

   Purpose

   promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P3 position (I/J)

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   (I) pGPD (J)
   

   Expression

   Bacterial

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. (S) insD_ku70 (Q) (SBE139)

   Plasmid

   #187627

   Purpose

   the insertional region downstream for the deletion of the KU70 gene in R. toruloides. Overhangs S/Q

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   (S) insD KU70 (Q)
   

   Expression

   Bacterial

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. (P) insUP_ku70 (SBE138) (B)

    Plasmid

    #187626

    Purpose

    the insertional region upstream for the deletion of the KU70 gene in R. toruloides. Overhangs P/B

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (P) insUP KU70 (B)
    

    Expression

    Bacterial

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-hsyn-dMASS_1_WPRE

    Plasmid

    #209765

    Purpose

    AAV virus production for neuronal expression of dMASS_1 under hSyn promoter

    Depositor

    Andre Berndt

    Article

    Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

    Insert

    dMASS_1
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYea5.1_Notch1_sfGFP

    Plasmid

    #207801

    Purpose

    Yeast 2µ vector for expression of Suc2SP-Notch1_100-sfGFP-GAL4

    Depositor

    Dieter Langosch

    Article

    Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

    Insert

    Suc2SP-Notch1_100-sfGFP-GAL4 fusion protein
    

    Tags

    Strep-TagII

    Expression

    Bacterial and Yeast

    Promoter

    TEF

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMx/ATP11C-c-HA

    Plasmid

    #209239

    Purpose

    Mammalian expression of ATP11C isoform c

    Depositor

    Hye-Won Shin

    Article

    Inoue et al J Cell Sci. 2021 Oct 15;134(20):jcs25852

    Insert

    ATP11C isoform c (ATP11C Human)
    

    Use

    Retroviral

    Tags

    HA

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMx/ATP11C-b-HA

    Plasmid

    #209238

    Purpose

    Mammalian expression of ATP11C isoform b

    Depositor

    Hye-Won Shin

    Article

    Inoue et al J Cell Sci. 2021 Oct 15;134(20):jcs25852

    Insert

    ATP11C isoform b (ATP11C Human)
    

    Use

    Retroviral

    Tags

    HA

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBAD-HisD-BR1

    Plasmid

    #192816

    Purpose

    Expresses BR1 in bacteria

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    BR1 (BRI1 Mustard Weed)
    

    Expression

    Bacterial

    Promoter

    araBAD promoter

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJAT58

    Plasmid

    #204304

    Purpose

    Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable 3XP3-DsRed cassette expressed in eyes.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    3XP3-DsRed
    

    Use

    CRISPR

    Promoter

    3XP3

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC57-min5UTR-hRluc-A60

    Plasmid

    #182638

    Purpose

    Transcription template plasmid for a minimal 5'UTR followed by Renilla luciferase open reading frame and polyA sequence

    Depositor

    Stephen Floor

    Article

    Calviello et al Nucleic Acids Res. 2021 May 21;49(9):533

    Insert

    Renilla luciferase,
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHcRed-CLCa

    Plasmid

    #196911

    Purpose

    visualization of clathrin-coated vesicle in living mammalian cells

    Depositor

    Kenji Sugimoto

    Article

    Sakaushi et al Biosci Biotechnol Biochem. 2007 Jul;71(7

    Insert

    Clathrin-Coated light chain
    

    Tags

    HcRed

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NeuA.pJL1

    Plasmid

    #199115

    Purpose

    In vitro expression of N-acylneuraminate cytodiylyltransfease (NeuA), which conjugates sialic acid and CTP to make activated sugar donor, CMP-sialic acid (see CSTI.PJL1 and PdST6.PJL1)

    Depositor

    Michael Jewett

    Article

    Thames et al ACS Synth Biol. 2023 Apr 11. doi: 10.102

    Insert

    N-acylneuraminate cytodiylyltransfease (NeuA)
    

    Tags

    Strep-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA.3-V5-SPOP (R221C)

    Plasmid

    #197022

    Purpose

    Mammalian expression vecor for V5-tagged SPOP

    Depositor

    Tanja Mittag

    Article

    Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

    Insert

    SPOP (Spop Mouse)
    

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R221C

    Available Since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCDNA.3-V5-SPOP (R354H)

    Plasmid

    #197023

    Purpose

    Mammalian expression vecor for V5 tagged SPOP

    Depositor

    Tanja Mittag

    Article

    Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

    Insert

    SPOP (Spop Mouse)
    

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R354H

    Available Since

    April 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pWzl_CRKL-W160L+W275L

    Plasmid

    #196021

    Purpose

    Expresses CRKL W160L + W275L

    Depositor

    William Hahn

    Article

    Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

    Insert

    CRKL
    

    Use

    Retroviral

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLenti6.3_CRKL-silent-V5

    Plasmid

    #196015

    Purpose

    Expresses CRKL-silent, V5 tagged

    Depositor

    William Hahn

    Article

    Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

    Insert

    CRKL
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLenti6.3_CRKL-W160L-V5

    Plasmid

    #196017

    Purpose

    Expresses CRKL W160L, V5 tagged

    Depositor

    William Hahn

    Article

    Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

    Insert

    CRKL
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. plx304_CRKL-open-V5

    Plasmid

    #196023

    Purpose

    Expresses CRKL open, V5 tagged

    Depositor

    William Hahn

    Article

    Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

    Insert

    CRKL
    

    Use

    Lentiviral

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pWzl_CRKL-W160L+Y207F

    Plasmid

    #196020

    Purpose

    Expresses Expresses CRKL W160L + Y207F

    Depositor

    William Hahn

    Article

    Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

    Insert

    CRKL
    

    Use

    Retroviral

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. His SUMO TEV R221C SPOP 28-359

    Plasmid

    #194116

    Purpose

    R221C His SUMO SPOP expression plasmid

    Depositor

    Tanja Mittag

    Article

    Thomasen et al Elife. 2023 Mar 1;12:e84147. doi: 10.755

    Insert

    SPOP R221C (SPOP Human)
    

    Tags

    6His-SUMO-TEV

    Expression

    Bacterial

    Mutation

    R221C

    Available Since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. FUiLMO2W

    Plasmid

    #129480

    Purpose

    3rd-generation lentiviral vector for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical

    Depositor

    Robert Gross

    Article

    Tung et al Sci Rep. 2015 Sep 24;5:14366. doi: 10.10

    Insert

    iLMO2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    human ubiquitin C

    Available Since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3-iLMO2

    Plasmid

    #129474

    Purpose

    Mammalian expression vector for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical

    Depositor

    Robert Gross

    Article

    Tung et al Sci Rep. 2015 Sep 24;5:14366. doi: 10.10

    Insert

    iLMO2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PH-miRFP-FRBx2

    Plasmid

    #193569

    Purpose

    Expresses PH domain from PLC delta1 tagged with miRFP703 and two FRB mutants (T2098L) in mammalian cells.

    Depositor

    Justin Taraska

    Article

    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

    Insert

    PH domain from PLC delta1 (Plcd1 Rat)
    

    Tags

    FRB (T2098L) and miRFP703

    Expression

    Mammalian

    Mutation

    PH domain

    Promoter

    CMV

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. PH-mCherry-FRBx2

    Plasmid

    #193570

    Purpose

    Expresses PH domain from PLC delta1 tagged with mCherry and two FRB mutants (T2098L) in mammalian cells.

    Depositor

    Justin Taraska

    Article

    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

    Insert

    PH domain from PLC delta1 (Plcd1 Rat)
    

    Tags

    FRB (T2098L) and mCherry

    Expression

    Mammalian

    Mutation

    PH domain

    Promoter

    CMV

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Rtn2b-NTD136-271-mNeon

    Plasmid

    #186604

    Purpose

    Encodes human Reticulon 2 isoform B N-terminal domain aa 136 to 271 fused to mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

    Insert

    Human Reticulon 2 isoform B amino acids 136 to 271 (RTN2 Human)
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET28-6xhis-sumo-CeMIGA(106-496)

    Plasmid

    #192832

    Purpose

    Express C.elegans MIGA fragment 106-496 in bacteria

    Depositor

    Karin Reinisch

    Article

    Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

    Insert

    Mitoguardin homologue (miga-1 Nematode)
    

    Tags

    6xhis sumo

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. LLP754_L2pV1_NOT_FRT_Rluc_1IF_lacZ

    Plasmid

    #192399

    Purpose

    Off state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-Rluc-FRT
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. LLP753_L2pV1_NOT_FRT_Rluc_0I_lacZ

    Plasmid

    #192398

    Purpose

    On state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-Rluc-FRT
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. LLP730_L2_pV01_0I_FRT-OCS-TB-rLUC_TCTP-fLUC

    Plasmid

    #192373

    Purpose

    To test for the repressive potential of the OCS and Transcription Block (TB) fusion with the FRT sites around it.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-TB-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. LLP733_L2_pV01_0I_FRT-uORF-rLUC_TCTP-fLUC

    Plasmid

    #192376

    Purpose

    To test for the repressive potential of the conserved peptide uORF from AT1G36730 with the FRT sites around it.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-uORF-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. LLP727_L2_pV01_0I_TMV-rLUC_TCTP-fLUC

    Plasmid

    #192370

    Purpose

    To control for variation introduced to the gene circuits, Rluc expression not affected by the introduction of terminators or recombination target sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. LLP732_L2_pV01_0I_FRT-35Ster-Rluc_lacZ

    Plasmid

    #192375

    Purpose

    To test for the repressive potential of the 35S terminator from CaMV with the FRT sites around it.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-35Ster-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. LLP731_L2_pV01_0I_FRT-OCStrunc-Rluc_lacZ

    Plasmid

    #192374

    Purpose

    To test for the repressive potential of the OCS variant (truncation) with the FRT sites around it.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCStrunc-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. LLP741_L2pV1_CR_B3RT-Rluc_TCTP-FlpO_lacZ

    Plasmid

    #192385

    Purpose

    To test for if Flp is able to de-repress a circuit with B3RT, to rule out cross-reactivity between these recombinases.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::B3RT-OCS-B3RT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. LLP742_L2pV1_CR_FRT-Rluc_TCTP-B3o_lacZ

    Plasmid

    #192386

    Purpose

    To test for if B3 is able to de-repress a circuit with FRT, to rule out cross-reactivity between these recombinases.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. LLP757_L2pV3_NOT_B3RT_Rluc_0I_lacZ

    Plasmid

    #192404

    Purpose

    On state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    B3RT-Act2-B3RT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. LLP756_L2pV1_NOT_B3RT_Rluc_1IB_lacZ

    Plasmid

    #192401

    Purpose

    Off state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::B3RT-Rluc-B3RT
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. LLP755_L2pV1_NOT_B3RT_Rluc_0I_lacZ

    Plasmid

    #192400

    Purpose

    On state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::B3RT-Rluc-B3RT
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. LLP714_L2pV3_NOT_FRT_Rluc_0I_lacZ

    Plasmid

    #192402

    Purpose

    On state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    FRT-Act2-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. LLP758_L2pV3_NOT_B3RT_Rluc_1IB_lacZ

    Plasmid

    #192405

    Purpose

    Off state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    B3RT-Act2-B3RT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. LLP713_L2pV3_NOT_FRT_Rluc_1IF_lacZ

    Plasmid

    #192403

    Purpose

    Off state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    FRT-Act2-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. LLP738_L2pV1_1I_Act2_FRT_OCS_s35S_Flp_lacZ

    Plasmid

    #192381

    Purpose

    To test if the 35S promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. LLP736_L2pV1_1I_Act2_FRT_OCS_TCTP_Flp_lacZ

    Plasmid

    #192379

    Purpose

    To test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output (despite the presence of the lacZ endlinker).

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge