Addgene

We narrowed to 21,570 results for: ESC

Showing: 6751 - 6800 of 21570 results

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pCWori-A13AMO-aaCPRct

Plasmid

#20117

Depositor

Jay Keasling

Article

Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

Insert

Amorphadiene oxidase and p450 reductase

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pRF-BetI

Plasmid

#49357

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

BetI repressor

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGL0_3 [RSF1010 ORI]

Plasmid

#198920

Purpose

Level 0 part

Depositor

Charlie Gilbert, Cultivarium Tools, Nili Ostrov

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

RSF1010 ORI

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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3xmNeonGreen_UtrCH

Plasmid

#129607

Purpose

In vivo visualization of F-actin (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

Utrophin

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pMUT2-pCadBA-mCherry

Plasmid

#192865

Purpose

pMUT2 based vector for sensing pH in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

pH sensitive promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTubulin-miRFP670-2

Plasmid

#197238

Purpose

Expresses the fusion protein of Tubulin-miRFP670-2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Tubulin-miRFP670-2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLSSmKate1-N1

Plasmid

#31866

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

LSS-mKate1

Use

Tags

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Promoter

CMV

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
mNeonGreen-peroxi

Plasmid

#137806

Purpose

Visualization of peroxisomes

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

SKL

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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mOrange2-C1

Plasmid

#54650

Purpose

Localization: C1 Cloning Vector, Excitation: 549, Emission: 565

Depositor

Michael Davidson, Roger Tsien

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Type

Empty backbone

Use

Tags

mOrange2

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pH2B-miRFP713-N1

Plasmid

#197230

Purpose

Expresses the fusion protein of H2B-miRFP713 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

H2B-miRFP713

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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GPI-mEGFP(2x)

Plasmid

#197911

Purpose

Homo-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-mEGFP-mEGFP

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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pHC79

Plasmid

#167551

Purpose

Backbone plasmid to pPM1001, pPM1002, pPM1003, pPM2212 and pPM2213

Depositor

Renato Morona

Article

Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

Type

Empty backbone

Use

Cosmid

Tags

Expression

Mutation

Promoter

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pEB1-SCFP3A

Plasmid

#103970

Purpose

Plasmid encoding SCFP3A

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

SCFP3A

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, S65T, …

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pSFFV_sfCherry2_TagBFP

Plasmid

#83031

Purpose

Expresses full length sfCherry2 and TagBFP fusion in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry+full length_TagBFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

3 mutations in sfCherry full length: E118Q, T128I…

Promoter

SFFV

Available since

Jan. 16, 2017

Availability

Academic Institutions and Nonprofits only

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DAGR

Plasmid

#14865

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

DAG reporter (Prkcb Rat)

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP E17TAG

Plasmid

#68295

Purpose

E17TAG GFP reporter

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

E17TAG GFP

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-iRFP670

Plasmid

#45453

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP670

Use

Tags

Expression

Bacterial

Mutation

T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

Promoter

araBAD

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pJRK138

Plasmid

#173746

Purpose

CeLINC plasmid

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Prps-0::CRY2(olig)::VHH(GFP)::unc-54 3' UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Prps-0

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pET-gSTEP1

Plasmid

#159376

Purpose

Expresses gSTEP1 in E. coli

Depositor

Roberto Chica

Article

Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

Insert

gSTEP1

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
mRFP-RBD(R59A)-mRFP

Plasmid

#18664

Depositor

Karel Svoboda

Article

Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

Insert

Ras binding domain of Raf

Use

Tags

mRFP

Expression

Mammalian

Mutation

R59A mutation in RBD

Promoter

Available since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB1C3-J23110-B0034-mRFP1_Violet

Plasmid

#160447

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Violet chromoprotein in E. coli

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Violet

Use

Synthetic Biology

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pNH051

Plasmid

#42157

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

GFP-RT-GFP

Use

Tags

Expression

Mutation

Promoter

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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p2lox-cAMPr Y205A

Plasmid

#102438

Purpose

cAMPr with a point mutation Y205A which should render PKA-C catalytically dead

Depositor

Justin Blau

Article

Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

Insert

cAMPr

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

PKA-C Y205A

Promoter

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTubulin-miRFP720

Plasmid

#197241

Purpose

Expresses the fusion protein of Tubulin-miRFP720 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Tubulin-miRFP720

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
C122-E11: eGFP

Plasmid

#162893

Purpose

Standard Gateway entry clone encoding eGFP fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

enhanced Green fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-HisD-miRFP680-HO1

Plasmid

#197253

Purpose

Expresses the protein of miRFP680 and HO1 in bacteria

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP680

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPEPZ-Plac

Plasmid

#122635

Purpose

BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae

Depositor

Jan-Willem Veening

Article

Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTn7-SCOUT11.B

Plasmid

#201005

Purpose

pLv1-pUC18R6KT-miniTn7-Gm-TagBFP, GmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

TagBFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23104

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-sgCDKN1B#2 BFP

Plasmid

#60905

Purpose

A lentiviral plasmid that encodes both a sgRNA targeting CDKN1B and BFP

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

BFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD/HisB-iRFP720

Plasmid

#45460

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP720

Use

Tags

Expression

Bacterial

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

Promoter

araBAD

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-TRE-mTurquoise2-WPRE

Plasmid

#104110

Purpose

AAV-mediated expression of mTurquoise2 under the TRE promoter.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

mTurquoise2

Use

AAV

Tags

Expression

Mutation

Promoter

TRE

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pPAmKate-N1

Plasmid

#34587

Depositor

Vladislav Verkhusha

Article

Gunewardene et al Biophys J. 2011 Sep 21;101(6):1522-8. Ep

Insert

PAmKate

Use

Tags

Expression

Mammalian

Mutation

F84W/S148N/S165G compared to mKate (but numbering…

Promoter

CMV

Available since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
EMTB-mScarletI

Plasmid

#137801

Purpose

Visualization of microtubules

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

Ensconsin microtubule binding domain

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBbi-BN

Plasmid

#60518

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and neomycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Tags

Expression

Mammalian

Mutation

Promoter

EF1a/RPBSA

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-HisD-miRFP720-HO1

Plasmid

#197257

Purpose

Expresses the protein of miRFP720 and HO1 in bacteria

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP720

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJDC182

Plasmid

#60854

Purpose

Expresses firefly luciferase

Depositor

Jeffrey Cirillo

Article

Chang et al PLoS One. 2014 Sep 29;9(9):e108341. doi:

Insert

Firefly luciferase (optimized for Mycobacteria)

Use

Tags

Expression

Bacterial

Mutation

codon optimized for Mycobacteria

Promoter

hsp60

Available since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-UXPS

Plasmid

#172136

Purpose

mammalian expression plasmid for a UDP/UTP sensor

Depositor

Huiwang Ai

Article

Li et al ACS Cent Sci. 2021 Oct 27;7(10):1763-177

Insert

UXPS

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pASR103

Plasmid

#122637

Purpose

BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae (pPEPZ Backbone)

Depositor

Jan-Willem Veening

Article

Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAH-pnGFP-Ultra

Plasmid

#157924

Purpose

Peroxynitrite sensor pnGFP-Ultra in mammalian cells (use with pMAH-POLY-eRF1(E55D))

Depositor

Huiwang Ai

Article

Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-

Insert

pnGFP-Ultra

Use

Tags

Expression

Mammalian

Mutation

Promoter

cmv

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
ITPKA_3xmNeonGreen

Plasmid

#129606

Purpose

In vivo visualization of F-actin (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

F-tractin

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-slpGFP

Plasmid

#27171

Depositor

Michela Lizier

Article

Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

Insert

surface (S)-layer protein promoter region fused with modified green fluorescent protein 5

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pGC4593

Plasmid

#110206

Purpose

Accessory plasmid with inducible TEV protease and unnatural tRNA system (AcF)

Depositor

Adam Arkin, Guillaume Cambray

Article

Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

Inserts

TEV protease
aaRS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pbad and Ptet

Available since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pH2B-LSSmOrange

Plasmid

#37133

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

Insert

LSSmOrange

Use

Tags

histone 2B

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-VSFP Butterfly 1.2

Plasmid

#47978

Purpose

Genetically encoded voltage indicator

Depositor

Thomas Knopfel

Article

Akemann et al J Neurophysiol. 2012 Oct;108(8):2323-37.

Insert

VSFP Butterfly 1.2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
GW1-ARSeNL

Plasmid

#207873

Purpose

Mammalian expression of the BRET ATP sensor

Depositor

Mathew Tantama

Article

Min et al Sensors (Basel). 2019 Aug 10;19(16). pii

Insert

ARSeNL

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pActinin-LSSmOrange

Plasmid

#37139

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

Insert

LSSmOrange

Use

Tags

human alpha-actinin

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO_Lifeact_HaloTag7

Plasmid

#175535

Purpose

Expression of HaloTag7 at the actin filaments of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Lifeact-HaloTag7

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPaxillin-PSmOrange

Plasmid

#31923

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Use

Tags

Paxillin

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-his6-HA3-SNAPf

Plasmid

#188941

Purpose

T7 expression vector for making his6- and HA3-tagged SNAP protein

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

his6-HA3-SNAPf

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSC101-GFPmut2(ΔmScarlet)

Plasmid

#208185

Purpose

GFP positive, mScarlet-I negative control

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Insert

GFPmut2

Use

Reporter

Tags

Expression

Bacterial

Mutation

Promoter

Ptet+dnaK P1

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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