We narrowed to 6,963 results for: RAP

1. L2379-FKBP-GPA(V84R)-20-11S

   Plasmid

   #222901

   Purpose

   Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 11S

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FKBP-GPA(V84R)-20GS-NanoLuc11S
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   V84R in GPA

   Promoter

   CMV

   Available since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. L2380-FKBP-GPA(V84R)-20-114

   Plasmid

   #222900

   Purpose

   Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 114

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FKBP-GPA(V84R)-20GS-NanoLuc114
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   V84R in GPA

   Promoter

   CMV

   Available since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. L2378-FRB-GPA(V84R)-20-11S

   Plasmid

   #222899

   Purpose

   Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA(V84R)-20GS-NanoLuc11S
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   V84R in GPA

   Promoter

   CMV

   Available since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. L2377-FRB-GPA(V84R)-20-114

   Plasmid

   #222898

   Purpose

   Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 114.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA(V84R)-20GS-NanoLuc114
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   V84R in GPA

   Promoter

   CMV

   Available since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. L2085-WT-GPA-NLuc-Biosensor-Retrovirus-TD135

   Plasmid

   #222875

   Purpose

   Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). WT Glycophorin A (GPA) scaffold.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA(WT)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV LTR

   Available since

   Aug. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. L2132-FKBP-GPA-114-5-CyOFP1

   Plasmid

   #222891

   Purpose

   Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 5 GS linker, CyOFP1 protein.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FKBP-GPA-20GS-NanoLuc114-5GS-CyOFP1
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. L2127-FRB-GPA-114-20-CyOFP1

   Plasmid

   #222886

   Purpose

   Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 20 GS linker, CyOFP1 protein.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-20GS-NanoLuc114-20GS-CyOFP1
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. L2129-FRB-GPA-114-5-CyOFP1

   Plasmid

   #222888

   Purpose

   Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 5 GS linker, CyOFP1 protein.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-20GS-NanoLuc114-5GS-CyOFP1
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. L2128-FRB-GPA-114-10-CyOFP1

   Plasmid

   #222887

   Purpose

   Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-20GS-NanoLuc114-10GS-CyOFP1
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. L2131-FKBP-GPA-114-10-CyOFP1

    Plasmid

    #222890

    Purpose

    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FKBP-GPA-20GS-NanoLuc114-10GS-CyOFP1
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. L2130-FKBP-GPA-114-20-CyOFP1

    Plasmid

    #222889

    Purpose

    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 20 GS linker, CyOFP1 protein.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FKBP-GPA-20GS-NanoLuc114-20GS-CyOFP1
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. APCDD1-P2A-tdTomato

    Plasmid

    #210801

    Purpose

    Fluorescent reporter for APCDD1 expression

    Depositor

    Yuejun Chen

    Article

    You et al Cell Stem Cell. 2023 Apr 6;30(4):473-487

    Insert

    APCDD1 (APCDD1 Human)
    

    Use

    Tags

    tdtomato

    Expression

    Mammalian

    Mutation

    Promoter

    endougeneous APCDD1 promoter

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-dCas12a-BE

    Plasmid

    #193648

    Purpose

    Expression of base editor dCas12a-BE in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-rAPOBEC1, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FKBP12-CB2mutx2-mTagBFP2-HAx2

    Plasmid

    #207147

    Purpose

    Tandem mutant calcium-buffer with rapalog dimerizer

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    Calbindin-2 (CALB2 Human)
    

    Use

    Tags

    FKBP12, HA, and mTagBFP2

    Expression

    Mammalian

    Mutation

    E40Q, E87Q, E131Q, E175Q, E219Q

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LentiU6-hIL1RN gRNA_1-MS2-Puro

    Plasmid

    #192677

    Purpose

    Lentiviral expression of sgRNA targeting hIL1RN promoter to activate human IL1RN transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human IL1RN activating gRNA #1 (IL1RN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. DHN_VrDHN1a_mCh-2xFKBP (pBS1117)

    Plasmid

    #185312

    Purpose

    For the mammalian expression of the riverbank grape plant protein DHN_VrDHN1a attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    DHN_VrDHN1a
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pST_DHN_VrDHN1a (or YSK2) (pBS0807)

    Plasmid

    #185266

    Purpose

    For the mammalian expression of the riverbank grape plant protein DHN_VrDHN1a (or YSK2). This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    DHN_VrDHN1a (or YSK2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FKBP-EGFP-RanGAP*

    Plasmid

    #175238

    Purpose

    Bacterial expression and purification, low affinity SUMOylation substrate that can recruited to FRB with rapamycin, point mutation F562A reduces affinity for E2, increasing the Km

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    RANGAP1 (RANGAP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Amino acid 562 F to A

    Promoter

    tac

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1(-)-HF2-BE3

    Plasmid

    #120397

    Purpose

    Expresses HF2-BE3 in mammalian cells

    Depositor

    Junjiu Huang

    Article

    Liang et al Protein Cell. 2017 Aug;8(8):601-611. doi

    Insert

    HF2-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mammalian codon-optimized Cas9-HF

    Promoter

    CMV

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. YFP-FKBP-DCRD

    Plasmid

    #87453

    Purpose

    rapamycin-induced membrane-targeting of DCRD

    Depositor

    Xiaodong Liu

    Article

    Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

    Insert

    DCRD (distal C-terminal regulatory domain) (CACNA1F Human)
    

    Use

    Tags

    6xHis-T7, FKBP, and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. YFP-FKBP-PCRD

    Plasmid

    #87452

    Purpose

    rapamycin-induced membrane-targeting of PCRD

    Depositor

    Xiaodong Liu

    Article

    Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

    Insert

    PCRD (proximal C-terminal regulatory domain) (CACNA1F Human)
    

    Use

    Tags

    FKBP and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. SRAcode_in_pUC57_v3(419-999)

    Plasmid

    #49824

    Purpose

    for transcribing SRA nucl 419-999 of NM_001035235 with T7

    Depositor

    Thomas Cech

    Article

    McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

    Insert

    steroid receptor RNA activator RNA coding (SRA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    KpnI site at 5' start of RNA coding seq

    Promoter

    T7

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. SRAcode_in_pUC57_v3(767-999)

    Plasmid

    #49825

    Purpose

    for transcribing SRA nucl 767-999 of NM_001035235 with T7

    Depositor

    Thomas Cech

    Article

    McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

    Insert

    steroid receptor RNA activator RNA coding (SRA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    KpnI site at 5' start of RNA coding seq

    Promoter

    T7

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. SRAcode_in_pUC57_v3(419-771)

    Plasmid

    #49823

    Purpose

    for transcribing SRA nucl 419-771 of NM_001035235 with T7

    Depositor

    Thomas Cech

    Article

    McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

    Insert

    steroid receptor RNA activator RNA coding (SRA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    KpnI site at 5' start of RNA coding seq

    Promoter

    T7

    Available since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Epac-S-H81

    Plasmid

    #170339

    Purpose

    GFPDel(UST)_EPACdDEPCD_mRFP biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.

    Insert

    GFPDel(UST)_EPACdDEPCD_mRFP (RAPGEF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA4/TO/GFP-TTP

    Plasmid

    #141190

    Purpose

    Expresses GFP-TTP in mammalian cells, can be used to make inducible cell line

    Depositor

    Simon Bekker-Jensen

    Article

    Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

    Insert

    TTP (ZFP36 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CARPID BASU-dCasRx

    Plasmid

    #153209

    Purpose

    express CARPID BASU-dCasRx fusion protein in mammalian cells

    Depositor

    Liang Zhang, Jian Yan

    Article

    Yi et al Nat Methods. 2020 Jun 22. pii: 10.1038/s

    Insert

    BASU-dCasRx
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    R239A/H244A/R858A/H863A in RfxCas13d

    Promoter

    EF-1a

    Available since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. opto-E-cad GFP

    Plasmid

    #203327

    Purpose

    Expresses E-cadherin with a LOV2 insert after T133 for blue light inhibition in mammalian cells

    Depositor

    Seraphine Wegner

    Article

    Nzigou Mombo et al Nat Commun. 2023 Oct 9;14(1):6292. doi:

    Insert

    Photoswitchable - E-cadherin with GFP tag (CDH1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Addition of Aslov2 domain at T133 of human E-cadh…

    Promoter

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTRE-BI-osTIR1

    Plasmid

    #207840

    Purpose

    Inducible TIR1 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

    Depositor

    Michaela Mueller-McNicoll

    Article

    Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

    Inserts

    osTIR
    mCherry
    mAID_-VHH-GFP4
    

    Use

    Tags

    3X HA-Tag and weak NLS

    Expression

    Mammalian

    Mutation

    All K mutated to R

    Promoter

    TRE3G BI promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3 Myc2- Hs TTP WT

    Plasmid

    #107008

    Purpose

    Expresses epitope-tagged wild-type human TTP

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    tristetraprolin (ZFP36 Human)
    

    Use

    Tags

    Double Myc peptide epitope tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHes1(467)-luc

    Plasmid

    #41723

    Depositor

    Ryoichiro Kageyama, Raphael Kopan

    Article

    Nishimura et al Genomics. 1998 Apr 1;49(1):69-75.

    Insert

    Hes1 Promoter (-467 to +46) (Hes1 Mouse)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Contains the murine Hes1 promoter (-467 to +46)

    Promoter

    Hes1 promoter fragment (-467 to +46)

    Available since

    March 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTRIPZ Myc2-mouse TTP WT

    Plasmid

    #107000

    Purpose

    Expresses epitope-tagged wild-type murine TTP

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    tristetraprolin (Zfp36 Mouse)
    

    Use

    Tags

    Double Myc peptide epitope tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Epac-S-H74

    Plasmid

    #170338

    Purpose

    mTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Klarenbeek et al PLoS One. 2011 Apr 29;6(4):e19170. doi:

    Insert

    mTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAc-C Med12His

    Plasmid

    #49240

    Purpose

    expresses human Med12 with His tag in insect cells

    Depositor

    Dylan Taatjes

    Article

    Knuesel et al Mol Cell Biol. 2009 Feb;29(3):650-61. do

    Insert

    Med12 (MED12 Human)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. SYKA-c020

    Plasmid

    #175489

    Purpose

    Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be used for crystallography.

    Depositor

    Opher Gileadi

    Article

    Bashore et al bioRxiv. 2023 Jul 29:2023.07.28.551026.

    Insert

    SYK (SYK Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. CARPID dCasRx-BASU

    Plasmid

    #153303

    Purpose

    express CARPID dCasRx-BASU fusion protein in mammalian cells

    Depositor

    Liang Zhang, Jian Yan

    Article

    Yi et al Nat Methods. 2020 Jun 22. pii: 10.1038/s

    Insert

    dCasRx-BASU
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    R239A/H244A/R858A/H863A in RfxCas13d

    Promoter

    EF-1a

    Available since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTRE-BI-aaAFB2

    Plasmid

    #207839

    Purpose

    Inducible AFB2 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

    Depositor

    Michaela Mueller-McNicoll

    Article

    Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

    Inserts

    atAFB2
    mCherry
    miniIAA7
    VHH-GFP4
    

    Use

    Tags

    3X FLAG-Tag and weak NLS

    Expression

    Mammalian

    Mutation

    All K mutated to R

    Promoter

    TRE3G BI promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEF-Bos TRAM Flag

    Plasmid

    #41551

    Depositor

    Kate Fitzgerald, Doug Golenbock

    Article

    Fitzgerald et al J Exp Med. 2003 Oct 6;198(7):1043-55. Ep

    Insert

    TRAM (TICAM2 Human)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

39. Epac-S-H90

    Plasmid

    #170340

    Purpose

    CFP(UST)_EPACdDEPCD_cp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.

    Insert

    CFP(UST)_EPACdDEPCD_cp173Venus(ST) (RAPGEF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLenti6/V5-DEST-ANLN

    Plasmid

    #31203

    Depositor

    Lynda Chin

    Article

    Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

    Insert

    ANLN (ANLN Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

41. pCAG-mCherry-FKBP-GAP(ARHGAP29)

    Plasmid

    #222632

    Purpose

    Rho inactivation using rapamycin

    Depositor

    Arnold Hayer

    Article

    Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

    Insert

    mCherry-FKBP-GAP(ARHGAP29) (ARHGAP29 Human)
    

    Use

    Tags

    mCherry-FKBP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pLD003-pCMV-APOBEC-Cas9(D10A)-rPB(8kD)

    Plasmid

    #165445

    Purpose

    Expresses ACX, rPB(8kD) in mammalian cells

    Depositor

    Wei Leong Chew

    Article

    Chen et al Nat Commun. 2021 Mar 2;12(1):1384. doi:

    Insert

    APOBEC-nCas9-rPB(8kD)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pTRE-BI-TRIM21

    Plasmid

    #207838

    Purpose

    Inducible TRIM21 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

    Depositor

    Michaela Mueller-McNicoll

    Article

    Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

    Inserts

    TRIM21 (TRIM21 Human)
    mCherry
    hIgG1-FC
    VHH-GFP4
    

    Use

    Tags

    weak NLS

    Expression

    Mammalian

    Mutation

    All K mutated to R

    Promoter

    TRE3G BI promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. TFORF2116

    Plasmid

    #144385

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NCOA6 (NCOA6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. TFORF0107

    Plasmid

    #143010

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TRERF1 (TRERF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pX459-HypaCas9-mR2-ANLN_sgRNA

    Plasmid

    #183876

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ANLN sgRNA for N-terminal tagging of anillin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ANLN sgRNA spacer (ANLN Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. LentiGuide-Puro-NCOA7g1 (BB22)

    Plasmid

    #139457

    Purpose

    Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. LentiGuide-Puro-NCOA7g2 (BB23)

    Plasmid

    #139458

    Purpose

    Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pTRF.1 udsVenus (P_JUND)

    Plasmid

    #58692

    Purpose

    A rapidly responsive reporter of endogenous JUND promoter activity (Lentiviral expression vector for reporter ultradestabilized Venus)

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    endogenous promoter of JUND (JUND Human)
    

    Use

    Lentiviral

    Tags

    udsVenus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

50. pcDNA5-eYFP-FKBP-MAD1-AA

    Plasmid

    #114038

    Purpose

    Chemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)

    Depositor

    Geert Kops

    Article

    Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

    Insert

    Human MAD1 (MAD1L1 Human)
    

    Use

    Tags

    eYFP-FKBP12

    Expression

    Mammalian

    Mutation

    MAD2 binding deficient MAD1 K541A/L543A

    Promoter

    CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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