We narrowed to 15,045 results for: NOL
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Plasmid#228290Purpose2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoter2,4-Diacetylphloroglucinol-regulatable synthetic …Available sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMM0646
Plasmid#129007PurposeIntegration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 markerDepositorInsertKanR-ColE1 URA 5'-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEL031
Plasmid#137900PurposeSTU2.0 SpyCas9 tRNA system for plant genome editingDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmIL-6 mut NF-kB
Plasmid#61293Purposedrives luciferase from mouse IL-6 promoter with mutation in NF-kB siteDepositorInsertIL-6 promoter (Il6 Mouse)
UseLuciferaseTagsExpressionMammalianMutationMutated NF-kB binding site from GTGGGATTTTCCCA to…PromoterIL-6 promoter with mutant NF-kB binding siteAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRDA_836
Plasmid#216088PurposeCas9 [Sp] knockout targeting CD55, positive controlDepositorInsertCD55 guide
UseCRISPR and Lentiviral; Positive controlsTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAP01
Plasmid#59981PurposeThe function of this material is to facilitate the capture and expression of bacterial gene clusters.DepositorTypeEmpty backboneUseSynthetic Biology; Transformation-associated reco…TagsnoExpressionBacterial and YeastMutationPromoternoAvailable sinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGWB516
Plasmid#74858PurposeGateway cloning compatible binary vector for C-terminal fusion with 4xMyc (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
A3A-PBE
Plasmid#119768PurposeConversion of C to T in wheat plants or monocotyledons protoplastsDepositorInsertAPOBEC3A-nCas9-NLS-UGI-NLS
UseTagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A and E945K in wild type …Promotermaize Ubiquitin-1Available sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
AF1521-c003
Plasmid#173111PurposeExpression of recombinant fusion protein in E. coliDepositorInsertAF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))
UseTagsNanolucExpressionBacterialMutationKH-M1-L192PromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-ChRger2-TS-EYFP
Plasmid#127238PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.DepositorInsertChRger2-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianMutationPromoterCamKIIaAvailable sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ166
Plasmid#109328PurposeGateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoterDepositorInsertzCas9
UseCRISPR; Gateway compatible zcas9 entry cloneTags3x FLAG, NLS and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL0341 (pTarget)
Plasmid#200906PurposeEncodes an mCherry casette under a minimal CMV promoter. Used as the target plasmid for plasmid-based integration assays, and for plasmid-based transcriptional activation.DepositorInsertpTarget
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-AOX1-eGFP
Plasmid#126718Purposeyeast plasmid overexpressing eGFPDepositorInsertegfp
UseTagsExpressionYeastMutationPromoterAOX1Available sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDUAL_tacI
Plasmid#155170PurposeBaSP and CuCBP co-expression: tacI promoter controls BaSP expressionDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57 APEX
Plasmid#40306PurposeA monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386).DepositorInsertpea APEX (Synthetic)
UseUnspecifiedTagsExpressionMutationK14D, W41F, E112K (relative to wild-type)PromoterAvailable sinceSept. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
TRE-TIGHT-EGFP-backward donor
Plasmid#22077DepositorInsertTETO-eGFP
UseTagsExpressionMutationPromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
UASGAL-Pgal
Plasmid#60338PurposeContains a promoter of activating sequence derived from native galactose promoter coupled with full length native galactose promoterDepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…TagsExpressionYeastMutationPromoterAvailable sinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMIII-Neo-BFP
Plasmid#153426PurposeRetroviral (MSCV) expression of mTagBFP2 fluorescent proteinDepositorInsertmTagBFP2
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJS646
Plasmid#183175PurposeY2H vector compatible with pJS647 PYR1 insertDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWB552
Plasmid#74882PurposeGateway cloning compatible binary vector for N-terminal fusion with G3GFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAF0474 Ef1a-Si74_attB acceptor
Plasmid#193475PurposeAcceptor plasmid for 3 plasmid recombination assayDepositorInsertSi74_attB
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMV_AA089
Plasmid#216105PurposegRNA expression for Cas12a (guide only)DepositorInsertgRNA expression
UseCRISPR and Lentiviral; Assembled vectorTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
ND4.2-Left TALE-G1397-N-DddA11
Plasmid#179681PurposeExpression of base editor in mammalian cellsDepositorInsertCOX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (S1330I A1341V N1342S E1370K T1380I)_1xUGI_ATP5B 3'UTR
UseTagsExpressionMammalianMutationS1330I A1341V N1342S E1370K T1380IPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AUG-nLuc-3XFLAG
Plasmid#127299PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codonDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/Envelope
Plasmid#120821PurposeEctopic expression of ZIKV proteinDepositorInsertEnvelope ZIKV
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
yTREX
Plasmid#177306Purposegenomic integration of genes (inserted in I-SceI-site) via transposon Tn5DepositorTypeEmpty backboneUseSynthetic Biology; Yeast expression, tn5 genomic …TagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
U6-5' MS2-CircRNA
Plasmid#213750PurposeFor circular RNA-mediated prime editor using U6-5' MS2-CircRNA in HEK293T cellsDepositorInsert5' ribozyme, 5' ligation sequences, 5' MS2, 3' ligation sequences, 3' ribozyme
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 IL6
Plasmid#67061PurposeStandard for cell-free expression benchmarking.DepositorInsertIL6 (IL6 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTK331
Plasmid#59572PurposeTC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceIDepositorInsertSGP(16) 2xK-turn Kozak EGFP attB2 AscI
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF-Bos MAL Flag
Plasmid#41554DepositorInsertMAL (TIRAP Human)
UseTags6xHis and FlagExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEK22
Plasmid#210588PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdH3pro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastMutationPromoterBdH3proAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
GWF243
Plasmid#133608PurposeNS3a-CAAX-(IRES)-EGFP-DNCR2-TIAM-P2a-BFP-GNCR1-LARGDepositorInsertNS3a-CAAX, EGFP-DNCR2-TIAM, BFP-GNCR1-LARG
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEL032
Plasmid#137901PurposeSTU2.0 LbCas12a for plant genome editingDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
attP-puro-GA donor
Plasmid#182140PurposeattP-puro-GA DNA donor for twinPE and Bxb1 mediated gene-size insertionDepositorInsertattP-GA-EGFP-EF1α-PuroR-T2A-TagBFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX-evoCas9
Plasmid#107550PurposeExpresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cellsDepositorInsertHumanized S. pyogenes Cas9
UseCRISPRTags3XFLAGExpressionMammalianMutationM495V/Y515N/K526E/R661QPromoterCBhAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRII-1.1-2.01 beta
Plasmid#69591PurposeOT-II TCR beta chain cDNA in pCRIIDepositorInsertTCR beta clone 1.1 (Tcrb Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a Gx-mNG-NL
Plasmid#169106PurposeExpresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - mNeonGreen - NanoLuc
UseTagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tight-Puro_Nkx2.2
Plasmid#64840PurposeA lentiviral vector for the expression of mouse Nkx2.2 under doxycyline control.DepositorInsertNkx2.2 (Nkx2-2 Mouse)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSecTag-ATX
Plasmid#17839DepositorInsertAutotaxin (ENPP2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSL2783 (pTnsC-VP64_Vch)
Plasmid#200896PurposeExpresses human codon-optimized V. cholerae TnsC-VP64 from a CMV promoter.DepositorInsertVchTnsC-VP64
UseTagsNLSExpressionMammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBK-YE1-BE3
Plasmid#85174PurposeExpresses YE1-BE3 in mammalian cellsDepositorInsertYE1-BE3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDUAL_2_araBAD
Plasmid#155176Purposesee pDUAL_araBAD: exchanged origin of replication (pBR322) and introduced bom siteDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pdCASclos
Plasmid#73640PurposeTranscriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824DepositorInsertsdCas9
sgRNA to spo0A
UseE.coli-clostridium shuttle vectorTagsExpressionMutationD10A, H840APromoterPj23119 and ptbAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
ABE8e-dimer
Plasmid#138490PurposeA-to-G base editorDepositorInsertecTadA(WT)-ecTadA(8e)-nSpCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDHblast-flx-sORF-Katushka
Plasmid#179457PurposeLentiviral vector encoding a CRE inducible Katushka geneDepositorInsertloxP-shuffledORF-loxP-Katushka
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMX-DivisionRecorder
Plasmid#179446PurposeRetroviral expression vector containing the DivisionRecorder cassetteDepositorInsertGFP-P2A-511-CrePI-511
UseRetroviralTagsExpressionMutationPromoterLTRAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWB435
Plasmid#74825PurposeGateway cloning compatible binary vector for C-terminal fusion with LUC (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_SCP1 vector
Plasmid#99292PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-(-30)GFP-9xGGS-Cre-6xHis
Plasmid#62372PurposeExpression of (-30)GFP-9xGGS-Cre-6xHis in bacterial cellsDepositorInsert(-30)GFP-9xGGS-Cre-6xHis
UseTags(-30)GFP and 6xHis tagExpressionBacterialMutationPromoterT7Available sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-YeNL
Plasmid#208849PurposeExpresses FLAG epitope-tagged YeNL "Yellow" BRET reporter in mammalian cells.DepositorInsertYeNL Nanolantern
UseLentiviralTags1xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only