We narrowed to 3,057 results for: bid

1. CaMKII-somBiPOLES-mCerulean

   Plasmid

   #154948

   Purpose

   Optogenetic tool for blue-light inhibition and red-light excitation of neurons

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Has Service

   AAV Retrograde, AAV5, and AAV9

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa(0.4)

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. natMX6-ins5

   Plasmid

   #195042

   Purpose

   pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

   Depositor

   Gloria Brar

   Article

   Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

   Insert

   NatR
   

   Use

   Yeast genomic targeting

   Tags

   S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PEX-mCherry-CRY2

   Plasmid

   #102250

   Purpose

   Expresses fusion of CRY2PHR with mCherry and PEX3 (1-42)

   Depositor

   Bianxiao Cui

   Article

   Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

   Insert

   PEX-mCherry-CRY2 (PEX3, CRY2 Mustard Weed, Human)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWZLblasti-HA-ERK2 GOF

   Plasmid

   #53174

   Purpose

   Expresses HA tagged human ERK2 R67SD321N from blasticidin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   ERK2 (MAPK1 Mustard Weed, Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   R67S/D321N

   Promoter

   Available since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pLNCX2-OptoTGFBRs

   Plasmid

   #118965

   Purpose

   Retroviral expression OptoTGFBRs for transient or stable constitutive expression

   Depositor

   Won Do Heo

   Article

   Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451

   Insert

   OptoTGFBRs (Tgfb1, Tgfbr2 Mouse, Mustard Weed)
   

   Use

   Retroviral

   Tags

   tdTomato

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. TRP1MX6-ins4

   Plasmid

   #195041

   Purpose

   pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

   Depositor

   Gloria Brar

   Article

   Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

   Insert

   TRP1
   

   Use

   Yeast genomic targeting

   Tags

   S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. CRY2clust-mCherry-RANK-P2A-CIBNcaax

   Plasmid

   #208613

   Purpose

   Mammalian expression of CRY2clust-mCherry-RANK-P2A-CIBNcaax (Opto-RANKm)

   Depositor

   Tomohiro Ishii, Takao Nakata

   Article

   Takada et al Sci Rep. 2024 Jan 19;14(1):1749. doi: 10

   Insert

   CRY2clust-mCherry-RANK-P2A-CIBNcaax (Cry2 Mouse, Mustard Weed)
   

   Use

   Tags

   CAAX and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cry2 mCherry GRK2 ct in pcDNA3.1

   Plasmid

   #64212

   Purpose

   mCherry fused between Cry2 and GRK2ct to study cell migration

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

   Inserts

   Cry2 PHR (CRY2 Mustard Weed)
   GRK2 ct (GRK2 Human)
   

   Use

   Tags

   mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and cmv

   Available since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pGEX4T3-HA-ERK2 K54R

   Plasmid

   #53170

   Purpose

   Expresses GST-HA tagged human ERK2 K54R from bacterial expression vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   ERK2 (MAPK1 Mustard Weed, Human)
   

   Use

   Tags

   GST and HA

   Expression

   Bacterial

   Mutation

   K54R

   Promoter

   Available since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pTEE

    Plasmid

    #159748

    Purpose

    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.

    Depositor

    Wolfgang Lukowitz

    Article

    Angulo et al bioRxiv

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCIBN-hTRF2-tagRFP-T

    Plasmid

    #103812

    Purpose

    BLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-hTRF2-tagRFP-T (CIB1, TERF2 Mustard Weed, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hTRF2: deletion of amino acids 1-42 and 476 compa…

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTUU

    Plasmid

    #159749

    Purpose

    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.

    Depositor

    Wolfgang Lukowitz

    Article

    Angulo et al bioRxiv

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. his3MX6-ins3

    Plasmid

    #195040

    Purpose

    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

    Depositor

    Gloria Brar

    Article

    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

    Insert

    His5+
    

    Use

    Yeast genomic targeting

    Tags

    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pWZLblasti-HA-ERK2 R67S

    Plasmid

    #53172

    Purpose

    Expresses HA tagged human ERK2 R67S from blasticidin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    ERK2 (MAPK1 Mustard Weed, Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R67S

    Promoter

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

15. Cry2 mCherry GRK3 ct in pcDNA3.1

    Plasmid

    #64213

    Purpose

    mCherry fused between Cry2 and GRK3 ct to study cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

    Inserts

    Cry2 PHR (CRY2 Mustard Weed)
    GRK3 ct (GRK3 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pWZLblasti-HA-ERK2 D321N

    Plasmid

    #53173

    Purpose

    Expresses HA tagged human ERK2 D321N from blasticidin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    ERK2 (MAPK1 Mustard Weed, Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D321N

    Promoter

    Available since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

17. INCTbiosyn-p35S-GFP(rc)13

    Plasmid

    #127526

    Purpose

    Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 13 attachment sites.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    EGFP reverse complement coding sequence flanked by attB/attP Integrase 13 attachment sites.
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-OptoFGFR1-Y766F

    Plasmid

    #59777

    Purpose

    Expresses optoFGFR1 with mutation in PLC-gamma binding phosphotyrosine site of FGFR1

    Depositor

    Won Do Heo

    Article

    Kim et al Chem Biol. 2014 Jul 17;21(7):903-12. doi

    Insert

    OptoFGFR1-Y766F (FGFR1 Mustard Weed, Human)
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PHR-GFP-FUSN-VP16

    Plasmid

    #183933

    Purpose

    Optogenetic PHR domain coupled to GFP, FUSN (high phase separation propensity) and VP16 activation domain; binds to CIBN upon blue light exposure

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    PHR-GFP-FUSN-VP16 (CRY2, UL48 Synthetic, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDGB3omega1_KanR_BastaR

    Plasmid

    #186426

    Purpose

    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar)

    Depositor

    Lukas Fischer

    Article

    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

    Inserts

    KanR
    BastaR
    

    Use

    Synthetic Biology; Binary vector for escherichia …

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    CaMV 35S and Pnos

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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