We narrowed to 803 results for: tev cleavage
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Plasmid#92237PurposeExpress H698A mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-H698A mutant
Tags6x-Histag, TEV cleavage siteExpressionBacterialMutationHistidine 698 to AlaninePromoterT7 promoterAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-R663A
Plasmid#92238PurposeExpress R663A mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-R663A mutant
Tags6x-Histag, TEV cleavage siteExpressionBacterialMutationArginine 663 to AlaninePromoterT7 promoterAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
JARID1BA-c539
Plasmid#79643PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertJARID1B (KDM5B Human)
UseBaculovirus expressionTags6x His and TEV cleavage sitePromoterpolyhedrinAvailable SinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
KRAS-A146T
Plasmid#154426PurposeA146T Kras with Flag tag inserted into N-terminal domain between 6Xhis tag and TEV cleavage site.DepositorInsertKRAS (KRAS Human)
Tags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialPromoterT5Available SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003
Plasmid#133447PurposeExpresses SpyCatcher003 in bacterial cytoplasmDepositorInsertSpyCatcher003
TagsGSSGS, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DogCatcher
Plasmid#171772PurposeExpresses DogCatcher protein in bacterial cytoplasmDepositorInsertDogCatcher
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJ404-DogCatcher-sfGFP
Plasmid#171930PurposeExpress DogCatcher-superfolder GFP protein in bacterial cytoplasmDepositorInsertDogCatcher-sfGFP
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT5Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oDi
Plasmid#124661PurposeExpresses SpyCatcher002-oDi for dimerization of SpyTag-proteins via dimeric coiled-coilDepositorInsertSpyCatcher002-oDi
TagsC-tag, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oTet
Plasmid#124663PurposeExpresses SpyCatcher002-oTet for tetramerization of SpyTag-proteins via tetrameric coiled-coilDepositorInsertSpyCatcher002-oTet
TagsC-tag, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oTri
Plasmid#124662PurposeExpresses SpyCatcher002-oTri for trimerization of SpyTag-proteins via trimeric coiled-coilDepositorInsertSpyCatcher002-oTri
TagsC-tag, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oHex
Plasmid#124670PurposeExpresses SpyCatcher002-oHex for hexamerization of SpyTag-proteins via hexameric coiled-coilDepositorInsertSpyCatcher002-oHex
TagsC-tag, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oPent
Plasmid#124664PurposeExpresses SpyCatcher002-oPent for pentamerization of SpyTag-proteins via pentameric coiled-coilDepositorInsertSpyCatcher002-oPent
TagsC-tag, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oHept
Plasmid#124671PurposeExpresses SpyCatcher002-oHept for heptamerization of SpyTag-proteins via heptameric coiled-coilDepositorInsertSpyCatcher002-oHept
TagsC-tag, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPROEX Aqua
Plasmid#42889DepositorInsertAquamarine
Tags6xHis and TEV cleavageExpressionBacterialMutationT65S and H148GAvailable SinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-sfGFP
Plasmid#184226PurposeExpresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.DepositorInsertSpyTag-sfGFP
TagsHis6, SpyTag, and TEV protease cleavage siteExpressionBacterialPromoterT5Available SinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Cys-SpyCatcher002
Plasmid#102829PurposeBacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagentsDepositorInsertCys-SpyCatcher002
TagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationCysteine residue in N-terminal regionPromoterT7Available SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
AviTag-SpyCatcher
Plasmid#72326PurposeBacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.DepositorInsertAviTag-SpyCatcher
TagsN-term His6 tag and TEV cleavage siteExpressionBacterialAvailable SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVTU_260_BirA
Plasmid#63682PurposeEncodes E. coli biotin ligase gene (BirA) for expression in yeast cellsDepositorInsertBirA
Tags6X Histidine and TEV cleavage siteExpressionYeastPromoterADH1/yeastAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Avitag-SpyCatcher002
Plasmid#102828PurposeBacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylationDepositorInsertAvitag-SpyCatcher002
TagsAvitag, GSSGS, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEL2108
Plasmid#201969PurposeORBIT integrating plasmid for C-terminal tagging with eGFP containing a 3C protease cleavage site.DepositorInsertattB-3C-eGFP-4xGly-TEV-Flag-6xHis
UseOrbit integrating plasmidAvailable SinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJCC_083 Cas12i1
Plasmid#171670PurposeFor bacterial expression of Cas12i1 with an N-terminal His-MBP tagDepositorInsertCas12i1
Tags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher002 EQ
Plasmid#102830PurposeBacterial expression of unreactive version of SpyCatcher002DepositorInsertSpyCatcher002 EQ
TagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationE77Q mutation prevents isopeptide bond formationPromoterT7Available SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-RGDSP-MMP
Plasmid#112633PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcherDepositorInsertTriCatcher-RGDSP-MMP
TagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral matrix metalloproteinase-cleavable sequen…PromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_CtCHAD
Plasmid#127785PurposeExpresses CtCHAD in E. coli cellsDepositorInsertCtCHAD (CT0884 Chlorobium tepidum)
Tags6xHis tag + TEV cleavage siteExpressionBacterialMutationResidues 208-522 (CHAD domain)Available SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_SsCHAD
Plasmid#127783PurposeExpresses SsCHAD in E. coli cellsDepositorInsertSsCHAD (SULM_RS11045 Sulfolobus solfataricus)
Tags6xHis tag + TEV cleavage siteExpressionBacterialAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
GRLF1
Plasmid#36195PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGRLF1 (Codon Devices Synthesized) (ARHGAP35 Human)
TagsHis tag with TEV cleavageExpressionBacterialMutationContains the Ras homolog domain only (amino acid …Available SinceJune 19, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
dFP
Plasmid#120542PurposeExpresses dFP biliverdin-binding fluorescence-activating full length protein in bacteria.DepositorInsertde novo fluorescence-activating protein (dFP)
Tags6xHis - TEV cleavageExpressionBacterialPromoterT7Available SinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_ygiF-FL
Plasmid#127795PurposeExpresses full-length ygiF in E. coli cellsDepositorAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 F124IHA
Plasmid#232993PurposeGalactose iduced expression of Gcn4 F124IHA in yeastDepositorInsertGcn4 F124I
Tags3xHA and TEV cleavage siteExpressionYeastMutationF124IPromoterGal1Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_FL Gcn4
Plasmid#232955PurposeGalactose iduced expression of full-length Gcn4 in yeastDepositorInsertGcn4
TagsTEV cleavage siteExpressionYeastPromoterGal1Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 W120AHA
Plasmid#232992PurposeGalactose iduced expression of Gcn4 W120AHA in yeastDepositorInsertGcn4 W120A
Tags3xHA and TEV cleavage siteExpressionYeastMutationW120APromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_DBDHA
Plasmid#232987PurposeGalactose iduced expression of Gcn4 DBDAHA in yeastDepositorInsertDBD
Tags3xHA and TEV cleavage siteExpressionYeastPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 L123A
Plasmid#232969PurposeGalactose iduced expression of Gcn4 L123A in yeastDepositorInsertGcn4 L123A
TagsTEV cleavage siteExpressionYeastMutationL123APromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Swap2
Plasmid#232965PurposeGalactose iduced expression of Gcn4 k0.19 in yeastDepositorInsertGcn4 k0.19
TagsTEV cleavage siteExpressionYeastPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Swap3
Plasmid#232960PurposeGalactose iduced expression of Gcn4 k0.47 in yeastDepositorInsertGcn4 k0.47
TagsTEV cleavage siteExpressionYeastPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 R2
Plasmid#232970PurposeGalactose iduced expression of Gcn4 R+ in yeastDepositorInsertGcn4 R+
TagsTEV cleavage siteExpressionYeastMutationK118R, K140RPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 E+
Plasmid#232963PurposeGalactose iduced expression of Gcn4 E+in yeastDepositorInsertGcn4 E+
TagsTEV cleavage siteExpressionYeastMutationD105E, D118E, D139EPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 W120A
Plasmid#232962PurposeGalactose iduced expression of Gcn4 W120A in yeastDepositorInsertGcn4 W120A
TagsTEV cleavage siteExpressionYeastMutationW120APromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 R3
Plasmid#232973PurposeGalactose iduced expression of Gcn4 R++ in yeastDepositorInsertGcn4 R++
TagsTEV cleavage siteExpressionYeastMutationD103R, D133R, K143RPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Swap3GFP
Plasmid#233010PurposeGalactose iduced expression of Gcn4 k0.47GFP in yeastDepositorInsertGcn4 k0.47
TagsHA-GFP-HA and TEV cleavage siteExpressionYeastPromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only