Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 5,803 results for: SUP

Clear keywords
Showing: 4261 - 4290 of 5803 results

  2. CD154-His-bio

    Plasmid
    #52337
    Purpose
    Expresses full-length Tumor necrosis factor ligand superfamily member 5 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    CD154 (CD40LG Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52337-plasmid-map-sequence-id-81058

    Enlarge

  3. Flag-M4-AR-S650D

    Plasmid
    #171246
    Purpose
    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser650Asp (AR-S650D)
    Depositor
    Steven Balk
    Article
    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do
    Insert
    3xFlag-tagged human androgen receptor Ser650Asp mutant (AR Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-Ser650Asp (AR-S650D)
    Promoter
    CMV
    Available Since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171246-plasmid-map-sequence-id-336097

    Enlarge

  4. Flag-M4-AR-S650A

    Plasmid
    #171245
    Purpose
    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser650Ala (AR-S650A)
    Depositor
    Steven Balk
    Article
    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do
    Insert
    3xFlag-tagged human androgen receptor Ser650Ala mutant (AR Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-Ser650Ala (AR-S650A)
    Promoter
    CMV
    Available Since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    171245-plasmid-map-sequence-id-336092

    Enlarge

  5. PB-Neo-TetO-Cdk12_Nterm_FlagHa

    Plasmid
    #127179
    Purpose
    N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector
    Depositor
    Phil Sharp
    Article
    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:
    Insert
    N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    Use
    Piggybac transposase vector
    Tags
    Flag- HA- Tandem Epitopes
    Expression
    Mammalian
    Promoter
    TetO Promoter
    Available Since
    July 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    127179-plasmid-map-sequence-id-247939

    Enlarge

  6. AAVS1-TetOn-3XFLAG-firefly-beta-globin-PTC39-AAVS1

    Plasmid
    #184397
    Purpose
    Donor plasmid for stable integration of firefly luciferase NMD(+) PTC39 reporter at AAVS1
    Depositor
    Robert Bradley
    Article
    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:
    Insert
    Firefly luciferase beta-globin-PTC39
    Use
    CRISPR; Donor plasmid for hdr
    Tags
    3X FLAG
    Expression
    Mammalian
    Mutation
    PTC in beta-globin at amino acid position 39
    Promoter
    Tet-On 3G
    Available Since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    184397-plasmid-map-sequence-id-362358

    Enlarge

  7. pLV_iRFP_P2AT2A_mScarlet-I_Myl9

    Plasmid
    #172474
    Purpose
    Mammalian expression of myosin light chain (Myl9) tagged with mScarlet-I and cytoplasmic iRFP as a reference marker.
    Depositor
    Sean R Collins
    Article
    Hadjitheodorou et al Nat Commun. 2021 Nov 16;12(1):6619. doi:
    Inserts
    iRFP670
    mScarlet-I-Myl9 (MYL9 Human)
    Use
    Lentiviral
    Tags
    mScarlet-I
    Expression
    Mammalian
    Promoter
    EF1alpha (with UCOE) and none (same transcript as…
    Available Since
    Sept. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    172474-plasmid-map-sequence-id-342797

    Enlarge

  10. AAVS1-TetOn-3XFLAG-renilla-beta-globin-PTC39-AAVS1

    Plasmid
    #184398
    Purpose
    Donor plasmid for stable integration of Renilla luciferase NMD(+) PTC39 reporter at AAVS1
    Depositor
    Robert Bradley
    Article
    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:
    Insert
    Renilla luciferase beta-globin-PTC39
    Use
    CRISPR; Donor plasmid for hdr
    Tags
    3X FLAG
    Expression
    Mammalian
    Mutation
    PTC in beta-globin at amino acid position 39
    Promoter
    Tet-On 3G
    Available Since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    184398-plasmid-map-sequence-id-362369

    Enlarge

  21. psiCHECK-VIM270-gSM

    Plasmid
    #215870
    Purpose
    This plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.
    Depositor
    Kumiko Ui-Tei
    Article
    Iribe et al ACS Omega. 2017 May 31;2(5):2055-2064. d
    Insert
    3 tandem repeats of target site (complementary to seed seq of guide strand of siRNA for VIM) is inserted to 3'UTR of RLuc (VIM Human)
    Expression
    Mammalian
    Available Since
    May 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    215870-plasmid-map-sequence-id-431352

    Enlarge

  22. psiCHECK-VIM270-gCM

    Plasmid
    #215868
    Purpose
    This plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.
    Depositor
    Kumiko Ui-Tei
    Article
    Iribe et al ACS Omega. 2017 May 31;2(5):2055-2064. d
    Insert
    target site which is completely complementary to guide strand of siRNA for vimentin is inserted to 3'UTR of RLuc (VIM Human)
    Expression
    Mammalian
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215868-plasmid-map-sequence-id-431664

    Enlarge

  28. CD40-bio-His

    Plasmid
    #51648
    Purpose
    Expresses full-length Tumor necrosis factor receptor superfamily member CD40 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    CD40 molecule (CD40 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51648-plasmid-map-sequence-id-78971

    Enlarge

  30. pAAV2-CAG-mScarlet-I-Cdt1

    Plasmid
    #191100
    Purpose
    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    mScarlet-I-Cdt1 (30-120)
    Use
    AAV and Synthetic Biology
    Tags
    mScarlet-I fused to residues 30-120 of human Cdt1…
    Expression
    Mammalian
    Mutation
    Optimized to human codon usage
    Promoter
    CMV enhancer and CAG
    Available Since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191100-plasmid-map-sequence-id-380122

    Enlarge

Showing: 4261 - 4290 of 5803 results