161,600 results

1. pMRX-INU-TEX264-FLAG

   Plasmid

   #128258

   Purpose

   Expresses TEX264 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

   Insert

   TEX264 (TEX264 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21a-TEX264-deltaLRR-His6

   Plasmid

   #220215

   Purpose

   Bacterial expression of TEX264, 2-25 residues (LRR) truncated, C-terminal His6 tag

   Depositor

   Kristijan Ramadan

   Article

   Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

   Insert

   TEX264 (TEX264 Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   c.4-75del / p.2-24del

   Promoter

   T7

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL4.23_Mmp11

   Plasmid

   #209520

   Purpose

   Expresses luciferase under control of Mmp11 promoter in transfected cells

   Depositor

   Jennifer Mitchell

   Article

   Khader et al PLoS One. 2024 Apr 18;19(4):e0297847. do

   Insert

   Mmp11 promoter (Mmp11 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-GFP-LC3-RFP-LC3ΔG

   Plasmid

   #168997

   Purpose

   Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Tags

   EGFP and mRFP1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUW-ubiquitin-EphB3-SV40-GFP (B3G)

   Plasmid

   #65443

   Purpose

   Expression of EPHB3 and GFP in mammalian cells

   Depositor

   Eduard Batlle

   Article

   Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

   Insert

   EPHB3 (EPHB3 Human)
   

   Use

   Lentiviral

   Tags

   SV40-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   UBQ

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. CMV-Mito4x-jRCaMP1b

   Plasmid

   #127873

   Purpose

   Red-shifted fluorescent reporter for mitochondrial matrix Ca2+

   Depositor

   Timothy Ryan

   Article

   Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

   Insert

   Red-shifted mitochondrial matrix Ca2+ indicator
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC015 - dLwCas13a-NF

   Plasmid

   #91905

   Purpose

   Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   dLwCas13a
   

   Use

   CRISPR

   Tags

   NLS and eGFP, ZF CCR5TC, KRAB-A, NLS

   Expression

   Mammalian

   Mutation

   R474A and R1046A

   Promoter

   CAG

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV_hSyn1_AchLightG

   Plasmid

   #187464

   Purpose

   Expresses green acetylcholine indicator AchLightG in neuronal cells

   Depositor

   Tommaso Patriarchi

   Article

   Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

   Insert

   AchLightG
   

   Use

   AAV

   Tags

   Igκ leader

   Expression

   Mutation

   Promoter

   human Synapsin-1

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV5 mSynCAM3

   Plasmid

   #115498

   Purpose

   expression of mouse full-length SynCAM 3

   Depositor

   Thomas Biederer

   Article

   Fogel et al J Neurosci. 2007 Nov 14;27(46):12516-30.

   Insert

   Cadm3 (Cadm3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-ESR1

    Plasmid

    #116737

    Purpose

    Lentiviral expression of ESR1

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ESR1 (ESR1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSCV-LMP1-IRES-mCherry

    Plasmid

    #220351

    Purpose

    Expresses EBV latent gene LMP1 in mammalian cells

    Depositor

    Klaus Rajewsky

    Article

    Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

    Insert

    LMP1 (LMP-1 Epstein-Barr Virus (EBV) strain B95-8)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pM201

    Plasmid

    #173179

    Purpose

    dCas9-24xGCN4

    Depositor

    Hajin Kim

    Article

    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

    Insert

    dCas9-24xGCN4
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF1alpha-rtTA-Antares

    Plasmid

    #197587

    Purpose

    Expresses TET-On reverse transactivator labeled with Antares

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    rtTA-P2A-Antares
    

    Use

    Tags

    Antares

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRRL-MHCK7-GCaMP6

    Plasmid

    #65042

    Purpose

    Lentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7

    Depositor

    Nenad Bursac

    Article

    Madden et al eLife. 2015 Jan 9;4:e04885. doi: 10.7554

    Insert

    GCaMP6
    

    Use

    Lentiviral

    Tags

    6xHis, T7 tag, and Xpress epitope

    Expression

    Mammalian

    Mutation

    Promoter

    MHCK7

    Available since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. TRE-UBC-Qa1 SCT-T2A-GFP

    Plasmid

    #205453

    Purpose

    lentiviral plasmid for expression of mouse Qa1 SCT

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    Qa1 SCT (H2-T23 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCG_SARS-CoV2 M C-V5-tag

    Plasmid

    #179978

    Purpose

    Mammalian expression vector for SARS-CoV-2 M, V5-tagged. Sequence codon-optimized.

    Depositor

    Konstantin Sparrer

    Article

    Hayn et al Cell Rep. 2021 May 18;35(7):109126.

    Insert

    SARS-CoV-2 M (M Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pME-puro-SNAP-FLAG-CD59

    Plasmid

    #50374

    Purpose

    Express SNAP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

    Depositor

    Reika Watanabe

    Article

    Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

    Insert

    SNAP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SR alpha

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-hSynapsin-iAchSnFR

    Plasmid

    #140644

    Purpose

    Acetylcholine sensor for lenti virus

    Depositor

    J. Julius Zhu

    Article

    Borden et al bioRxiv 2020

    Insert

    Ach sensor V9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hsynapsin

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. eGFP-proα2(I)-L

    Plasmid

    #120160

    Purpose

    Expresses mouse Type I procollagen α2 chain (Col1a2) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Article

    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

    Insert

    Type I procollagen α2 chain (Col1a2 Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Col1a2 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. TVBB C-term-mScarlet-Puro

    Plasmid

    #169220

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Puro at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mScarlet-P2A-Puro
    

    Use

    Targeting vector backbone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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