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170,787 results

Showing: 2861 - 2880 of 170787 results

  4. EF1a_mCherry_P2A_Hygro

    Plasmid
    #135003
    Purpose
    Lentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site.
    Depositor
    Prashant Mali
    Article
    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    135003-plasmid-map-sequence-id-272070

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  5. pMIG-w

    Plasmid
    #12282
    Purpose
    a bicistronic IRES-GFP containing vector which is a variant of pMIG that contains a posttranscriptional regulatory element from the woodchuck hepatitis virus
    Depositor
    Luk Parijs
    Article
    Refaeli et al J Exp Med. 2002 Oct 7. 196(7):999-1005.
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    IRES GFP
    Expression
    Mammalian
    Available Since
    July 28, 2006
    Availability
    Academic Institutions and Nonprofits only
    12282-plasmid-map-sequence-id-502075

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  6. pICE-FLAG-NAT10-siR-WT

    Plasmid
    #59365
    Purpose
    Plasmid for constitutive or doxycycline-inducible expression of wild-type human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Steve Jackson
    Article
    Larrieu et al Science. 2014 May 2;344(6183):527-32. do
    Insert
    human NAT10 (NAT10 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Mutations to render cDNA resistant to a siRNA
    Promoter
    CMV-tet
    Available Since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    59365-plasmid-map-sequence-id-92043

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  11. pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)

    Plasmid
    #140001
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9-NG with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…
    Promoter
    CAG
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140001-plasmid-map-sequence-id-477414

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  14. mCherry-TFR-20

    Plasmid
    #55144
    Purpose
    Localization: Transferrin Receptor, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    TFR (TFRC Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55144-plasmid-map-sequence-id-132722

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  15. pLV-dCas9-KRAB-PGK-HygR

    Plasmid
    #83890
    Purpose
    Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.
    Depositor
    Charles Gersbach
    Article
    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038
    Inserts
    humanized dead Cas9 KRAB
    aminoglycoside phosphotransferase from E. coli
    Use
    CRISPR and Lentiviral
    Tags
    Flag
    Mutation
    D10A and H840A
    Promoter
    Human Ubiquitin C Promoter and mouse phosphoglyce…
    Available Since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    83890-plasmid-map-sequence-id-240902

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  18. pCAG-VSVL

    Plasmid
    #64085
    Purpose
    Expression vector for Vesicular Stomatitis Virus Indiana strain polymerase gene
    Depositor
    Ian Wickersham
    Insert
    VSV polymerase
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    64085-plasmid-map-sequence-id-429028

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  20. pCMV-PEmax-P2A-hMLH1dn

    Plasmid
    #174828
    Purpose
    Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)
    Depositor
    David Liu
    Article
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Insert
    PEmax-P2A-hMLH1dn
    Tags
    SV40 bpNLS and c-Myc NLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript; deletion of residues 754-…
    Promoter
    CMV
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174828-plasmid-map-sequence-id-357934

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Showing: 2861 - 2880 of 170787 results