We narrowed to 14,669 results for: EGF
-
Plasmid#105812PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with C-terminal mCherry.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsTEV-mCherryExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only
-
MTOR-F2108L_CD19-CAR-28z-2A-EGFP_AAV6_Donor
Plasmid#211904PurposeVector for AAV6 donor production. Targeted CD19-CAR-28z-2A-EGFP transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.DepositorInsertCD19-CAR-28z-2A-EGFP
UseAAVExpressionMammalianPromoterhPGK1Available SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-TRE3G-NLS-tdPCP-EGFP
Plasmid#99910PurposeFor the expression of NLS-tPMCP-EGFPDepositorInsertNLS-tdPCP-EGFP
UseLentiviralExpressionMammalianPromoterTRE4GAvailable SinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-sCAG-eGFP-ACAP1 F280E
Plasmid#203801PurposeAAV vector plasmid expressing human ACAP1 mutation F280E fused to eGFP under a short variant of the CAG (sCAG) promoterDepositorAvailable SinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
SS80 pEGFPN1 anti-SAPAP2 nanobody
Plasmid#135228PurposeMammalian expression of anti-SAPAP (Rat) recombinant llama nanobody for use as an intrabodyDepositorInsertanti-SAPAP2 (Rat) recombinant llama nanobody SS80 (Dlgap2 L. glama (llama))
Tags6xHis-HA-EGFPExpressionMammalianAvailable SinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
Pdgfra-T2A-H2B-eGFP donor
Plasmid#113120PurposeDonor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Pdgfra coding sequenceDepositorInsertPdgfra donor region including homology arms and T2A-H2B-eGFP (Pdgfra Mouse, Synthetic)
UseSynthetic BiologyAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synP-FLEX-TVA66T-EGFP
Plasmid#64096PurposeExpresses TVA66T-EGFP under the synapsin promotorDepositorInsertTVA66T-EGFP
UseAAV and Cre/LoxTagsEGFPExpressionMammalianMutationGlutamic acid 66 to ThreoninePromotersynapsinAvailable SinceApril 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-VEGF(Brolucizumab) PAGER(Gq)
Plasmid#230014PurposeExpresses anti-VEGF PAGER(Gq) in mammalian cellDepositorInsertMT1-Brolucizumab-TEVcs-ALFA-hM1D
UseAAVExpressionMammalianMutationW442A on hM1DPromoterCMVAvailable SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-VEGF(Nb35) PAGER(FL)
Plasmid#230025PurposeExpresses anti-VEGF PAGER(FL) in mammalian cellsDepositorInsertpAAV-MT3-Nb35-TEVcs-ALFA-hM4DxcpGFP
UseAAVExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
Designed VEGF-binding scFv G6des13
Plasmid#110213PurposeYeast Surface Display of G6des13DepositorInsertG6des13
Tagscmyc tagExpressionYeastMutationL A43P, L Q89L, L T94D, L P95T, H Y59H, H Y95FAvailable SinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)
Plasmid#160140PurposeCMV and T7 promoter expression plasmid for human codon optimized AsCas12a with a c-terminal NLS(nucleoplasmin), 3x HA tag, and P2A-EGFPDepositorInserthuman codon optimized AsCas12a with NLS(nucleoplasmin)-3xHA-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsNLS(nucleoplasmin)-3xHA-P2A-EGFPExpressionMammalianPromoterCMV and T7Available SinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EGFP-T2A-ACREB-WPRE
Plasmid#40867DepositorInsertACREB
UseAAVPromoterCMVAvailable SinceNov. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGFP/RFP-miR-Scrint
Plasmid#19821DepositorInsertScrambled miRNA
UseCre/Lox and RNAiExpressionMammalianAvailable SinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pNL(CMV)EGFP/CMV/WPRE∆U3
Plasmid#41790PurposeLentiviral vector for CMV promoter driven expression of EGFPDepositorInsertEGFP
UseLentiviralAvailable SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MCS-eDHFR(69K6)-EGFP
Plasmid#172101PurposeMammalian expression of a protein of interest fused to the N-terminus of eDHFR(69K6)-EGFPDepositorInsert22aa-eDHFR(69K6)-EGFP
ExpressionMammalianMutationeDHFR(69K6): Hexalysine (K6) sequence is inserted…PromoterCMVAvailable SinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS NL4-3 envFS eGFP IN-D116A
Plasmid#101333PurposepBS NL4-3 envFS eGFP with mutation that disrupts IN catalytic activityDepositorInsertNL4-3 envFS
UseLentiviralMutationIntegrase D116AAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PDGFRb559-562del-IRES2-EGFP
Plasmid#204355PurposeExpresses a mutant PDGFRb gene (559-562 deletion).DepositorInsertPlatelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)
Mutation559-562 deletionAvailable SinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-SPdCas9-EGFP-2A-Blast
Plasmid#71215PurposedCas9 fused to EGFPDepositorInsertdSpCas9-EGFP
UseLentiviralTagsdSpCas9-EGFPExpressionMammalianAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3G_Puro-T2A-EGFP-StrepTagII-ProtC-HA
Plasmid#162089PurposeEGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; EGFP linked to StrepTagII-ProtC-HA
UseLentiviralTagsStrepTagII-ProtC-HAMutationWTPromoterEF1AAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3G_Puro-T2A-EGFP-VSVg-StrepTagII-HA
Plasmid#162080PurposeEGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; EGFP linked to VSVg-StrepTagII-HA
UseLentiviralTagsVSVg-StrepTagII-HAMutationWTPromoterEF1AAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
lenti-EGFR-L858R-nick sgRNA
Plasmid#214097PurposeLentiviral vector expressing nicking sgRNA to induce EGFR L858R mutation using PE3DepositorInsertEGFR L858R nicking sgRNA
UseLentiviralExpressionMammalianPromoterhU6Available SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-EGFR-T790M-nick sgRNA
Plasmid#214098PurposeLentiviral vector expressing nicking sgRNA to induce EGFR T790M mutation using PE3DepositorInsertEGFR T790M nicking sgRNA
UseLentiviralExpressionMammalianPromoterhU6Available SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS NL4-3 envFS eGFP Δrev
Plasmid#101348PurposeAll mutations in rev are silent with respect to the tat reading frame. Start ATG->ACG and nts 68-71 were mutated AGC->TCA to change tyrosine to a stop.DepositorInsertNL4-3
UseLentiviralMutationdelta revAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CBh-DIO-CB2-EGFP
Plasmid#91946PurposeExpression of mouse CB2 cannabinoid receptors and eGFP in the presence of CreDepositorInsertsEGFP
type-2 cannabinoid receptor
UseAAVTagsFLAG and NoneAvailable SinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synP-FLEX-splitTVA-EGFP
Plasmid#52474PurposeExpresses splitTVA-EGFPDepositorInsertsplitTVA-P2A-EGFP
UseAAV and Cre/LoxExpressionMammalianPromoterSynapsin-1Available SinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
Designed VEGF-binding scFv G6des1
Plasmid#110212PurposeYeast Surface Display of G6des1DepositorInsertG6des1
Tagscmyc tagExpressionYeastMutationL A43P, L Y49F, L Y55F ,L Q89L, L T94V, L P95T, L…Available SinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBac{3XP3::EGFP, Pactin::Ptrsps}
Plasmid#86861PurposeExpresses EGFP in eyes and ubiquitous expression of P-transposaseDepositorInsertpACNNTNPII-S129A
ExpressionInsectAvailable SinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
ZIF-NLS-EGFP(Y66F)-HOTag6
Plasmid#208749Purposepart B of nls-SparkDrop with non-fluorescent eGFPDepositorInsertZIF-NLS-EGFP(Y66F)-HOTag6
ExpressionMammalianAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3XFLAG-EGFP-NLS hygro
Plasmid#87781PurposeInducible expression of EGFP with a C-terminal NLS sequenceDepositorInserteGFP
UseLentiviralTags3xFLAG and NLSExpressionMammalianAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
C232-E05: attB2r-meGFP-attB3
Plasmid#162902PurposeGateway attB2r/attB3 entry clone for construction of C-terminal eGFP fusion proteinsDepositorInsertmonomeric enhanced Green fluorescent protein
UseSynthetic BiologyAvailable SinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
p87AAVEGFP hp47phox (S303/304/328D)
Plasmid#112250Purposemutant p47phoxDepositorAvailable SinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS6-ZF(VEGFA)-StaPL(TI)-tdRFP-KRAB
Plasmid#111504PurposeExpresses a zinc finger specific to the human VEGFA locus, which is linked to a KRAB transcriptional repression domain via a StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited].DepositorInsertZF(VEGFA)-StaPL(TI)-tdRFP-KRAB
ExpressionMammalianMutationThe HCV NS3 protease carries F43L, Q80K, S122R, a…PromoterCMVAvailable SinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-EGFP-T2A-RPL22-1xFlag
Plasmid#170323PurposeExpresses FLAG and EGFP tagged RPL22DepositorAvailable SinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2KXIGdeltaIn-MCS-Avi-EGFP-rpl10a
Plasmid#58380Purposetol2 transgenesis for biotin based TRAP - Avi tagged RibosomeDepositorInsertavi-EGFP-rpl10a
TagsAvi Tag and EGFPAvailable SinceAug. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT
Plasmid#216763PurposePiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposaseDepositorInsertmCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT
TagsEGFP and mCherryExpressionMammalianAvailable SinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
p{Mhc-F4-678-eGFP::FRT,attB}
Plasmid#182185Purposefluorescent reporter gene with yeast recombination site within intronDepositorInsertEGFP
ExpressionInsectAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-TRE-DsRed-IRES-d24EGFP
Plasmid#41945PurposeRetroviral expression of DsRed in mammalian cellsDepositorInsertDsRed
UseRetroviralExpressionMammalianPromoterTREAvailable SinceMay 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1α-IRES-EGFP-hMCIDAS
Plasmid#149696PurposeExpresses untagged human MCIDAS in mammalian cellsDepositorAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKK-RNAi-nucCHERRYmiR-EGFP-TEV
Plasmid#105810PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal EGFP.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsEGFP-TEVExpressionMammalianAvailable SinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Cep170 C-term (Nigg GG2)
Plasmid#41151DepositorInsertCep170 (CEP170 Human)
TagseGFPExpressionMammalianMutationContains C-terminal fragment (aa 755-1460, from X…PromoterCMV, T7Available SinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only