We narrowed to 14,580 results for: pET

1. UbcH5c WT pET28a

   Plasmid

   #12643

   Depositor

   Rachel Klevit

   Article

   Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

   Insert

   UbcH5c - Ubiquitin conjugating enzyme (UBE2D3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 31, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET32a-hEPR

   Plasmid

   #199233

   Purpose

   Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

   Depositor

   Nilson Zanchin

   Article

   Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

   Insert

   Proepiregulin preproprotein (EREG Human)
   

   Use

   Tags

   TrxA-6xHis-S-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET15TV-L-hlPYK WT

   Plasmid

   #187975

   Purpose

   Expresses the human liver pyruvate kinase

   Depositor

   Audrey Lamb

   Article

   McFarlane et al Acta Crystallogr F Struct Biol Commun. 2

   Insert

   hlPYK WT
   

   Use

   Tags

   Hexahistag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. NylCA-TS_pET28a

   Plasmid

   #215419

   Purpose

   Expression construct for NylCA-TS gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylCA-TS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S111G/A137L/E263Q

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. NylCp2-TS_pET28a

   Plasmid

   #215421

   Purpose

   Expression construct for NylCp2-TS gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylCp2-TS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D122G/H130Y/D36A/E263Q

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET24b-BR1.4

   Plasmid

   #199436

   Purpose

   expresses BAmRed1.4 in bacterial cells

   Depositor

   Andrew Woolley

   Article

   Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

   Insert

   BAMRed1.4
   

   Use

   Tags

   his tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pETCON-HtsptLB12v.3

   Plasmid

   #45121

   Depositor

   David Baker

   Article

   Procko et al J Mol Biol. 2013 Sep 23;425(18):3563-75.

   Insert

   HtsptLB12v.3
   

   Use

   Tags

   Aga2p for yeast surface display and c-myc epitope…

   Expression

   Yeast

   Mutation

   Promoter

   GAL

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET24b-Amg2

   Plasmid

   #199434

   Purpose

   expresses Amg2 in bacterial cells

   Depositor

   Andrew Woolley

   Article

   Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

   Insert

   Amg2
   

   Use

   Tags

   his tag

   Expression

   Bacterial

   Mutation

   L159K mutation

   Promoter

   T7

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28HIS-hAPE1

   Plasmid

   #70757

   Purpose

   bacterial expression of human APE1

   Depositor

   Primo Schaer

   Article

   Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

   Insert

   APEX1 (APEX1 Human)
   

   Use

   Tags

   6-HIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7/lac operon

   Available since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET11a-AldoA

    Plasmid

    #224925

    Purpose

    Inducible expression of human aldolase A

    Depositor

    Liskin Swint-Kruse

    Article

    Fenton et al Protein Sci. 2022 Feb;31(2):357-370. doi

    Insert

    Aldolase A (ALDOA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET32a-hTGFa

    Plasmid

    #199236

    Purpose

    Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

    Depositor

    Nilson Zanchin

    Article

    Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

    Insert

    Transforming growth factor alpha, partial (TGFA Human)
    

    Use

    Tags

    TrxA-6xHis-S-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHLSec2-FN-YPet

    Plasmid

    #65421

    Purpose

    Expresses in mammalian cells human plasma fibronectin with YPet inserted between FNIII domains 6 and 7

    Depositor

    Harold Erickson

    Article

    Ohashi et al J Biol Chem. 2011 Nov 11;286(45):39188-9

    Insert

    human plasma fibronectin with YPet inserted between FNIII domains 6 and 7 (FN1 Human)
    

    Use

    Tags

    YPet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET50_WT MDA5

    Plasmid

    #216802

    Purpose

    Expresses wild type (wt) melanoma differentiation-associated protein 5

    Depositor

    Sun Hur

    Insert

    melanoma differentiation-associated protein 5 (IFIH1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET11a-SUMO1

    Plasmid

    #53138

    Purpose

    E.coli expression of untagged mature human SUMO1 (amino acids 1-97)

    Depositor

    Frauke Melchior

    Article

    Pichler et al Cell. 2002 Jan 11;108(1):109-20.

    Insert

    SUMO1 (SUMO1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mature form (amino acids 1-97)

    Promoter

    T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET15b-Opt13

    Plasmid

    #61698

    Purpose

    Expresses PTDH mutant Opt13

    Depositor

    Huimin Zhao

    Article

    McLachlan et al Biotechnol Bioeng. 2008 Feb 1;99(2):268-

    Insert

    Opt13
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 3, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pET-2(KW)2-mCherry

    Plasmid

    #112738

    Purpose

    Express 2(KW)2-mCherry in E. coli

    Depositor

    Kyubong Jo

    Article

    Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6.

    Insert

    2(KW)2-mCherry
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET30-RT1306

    Plasmid

    #131521

    Purpose

    Expresses engineered RT-1306 with His-tag

    Depositor

    Bryan Dickinson

    Article

    Zhou et al Nat Methods. 2019 Dec;16(12):1281-1288.

    Insert

    RT-1306
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET29a-hNABP1

    Plasmid

    #128306

    Purpose

    Express hNABP1 (a.k.a hSSB2 or SOSSB2) in E. coli with a C-terminal His-tag

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

    Insert

    Human nucleic acid-binding protein 1 (hNABP1) (NABP1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21b-Caspase-3

    Plasmid

    #90087

    Purpose

    Expresses wild type caspase 3 in bacterial cells

    Depositor

    Clay Clark

    Article

    Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

    Insert

    Caspase 3 (CASP3 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Wild type

    Promoter

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-scFv-T

    Plasmid

    #67843

    Purpose

    pET-30-based vector dedicated to efficient scFv expression, which circumvents the problem of in-frame amber codons in scFvs.

    Depositor

    Joanna Bereta

    Article

    Ossysek et al Immunol Lett. 2015 Jul 25. pii: S0165-24

    Insert

    antibody 1D8 light kappa chain
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCDB179-DepoPETase

    Plasmid

    #218702

    Purpose

    Encodes codon optimized DepoPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Insert

    DepoPETase
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET21a Ov-iPGM

    Plasmid

    #180245

    Purpose

    Bacterial expression plasmid for production of recombinant Onchocerca volvulus iPGM His10

    Depositor

    James Inglese

    Article

    Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

    Insert

    Onchocerca volvulus iPGM-10His
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET21a Wb-iPGM

    Plasmid

    #180246

    Purpose

    Bacterial expression plasmid for production of recombinant Wuchereria bancrofti iPGM His10

    Depositor

    James Inglese

    Article

    van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

    Insert

    Wuchereria bancrofti iPGM-10His
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET15-UbE2D2

    Plasmid

    #15783

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2D2 (UBE2D2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

25. pET32-MCM2/7

    Plasmid

    #116949

    Purpose

    Expresses human MCM2 and human MCM7

    Depositor

    James Chong

    Article

    Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

    Inserts

    Human MCM7 (MCM7 Human)
    Human MCM2 (MCM2 Human)
    

    Use

    Tags

    Thioredoxin, His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET_RTX-6xHis

    Plasmid

    #145029

    Purpose

    pET vector for IPTG-inducible, T7-based expression of RTX bearing a C-terminal 6xHis tag

    Depositor

    Andrew Ellington

    Article

    Bhadra et al BioProtocol 2021

    Insert

    RTX
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TS181_pETNKI_his3C_pA-Dam

    Plasmid

    #200721

    Purpose

    producing pA-DamID methylation protein in bacteria

    Depositor

    Bas van Steensel

    Article

    van Schaik et al Methods Mol Biol. 2022;2458:215-229. doi

    Insert

    His3C-pA-Dam
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized

    Promoter

    Available since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28-SaCas9

    Plasmid

    #178901

    Purpose

    SaCas9 (Cas9 from Staphylococcus aureus)

    Depositor

    Hendrik Milting

    Article

    Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

    Insert

    SaCas9
    

    Use

    Tags

    His6-Tag, bipartite nuclear localization signal f…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET21a-C2-SNAP

    Plasmid

    #208769

    Purpose

    To express C2-SNAP in bacteria

    Depositor

    Urte Neniskyte

    Article

    Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

    Insert

    C2-SNAP (Mfge8 Mouse)
    

    Use

    Tags

    SNAP-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28a-T4uvsW

    Plasmid

    #228201

    Purpose

    Expression in BL21(DE3) of T4 3' to 5' helicase/single-strand DNA annealase

    Depositor

    Stephen Benkovic

    Article

    Nelson et al J Biol Chem. 2007 Jan 5;282(1):407-16. d

    Insert

    uvsW
    

    Use

    Tags

    hexahistidine and 10 amino acid linker

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET15b-mISG15(GG)

    Plasmid

    #12448

    Depositor

    Dong-Er Zhang

    Article

    Kim et al Methods Enzymol. 2005 . 398():491-9.

    Insert

    C-terminal processed form of ISG15
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

32. PE-Max-pet21A

    Plasmid

    #204471

    Purpose

    Expression plasmid for PE protein in bacteria.

    Depositor

    Scot Wolfe

    Article

    Ponnienselvan et al Nucleic Acids Res. 2023 May 29:gkad456.

    Insert

    Prime editor
    

    Use

    CRISPR

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET28a-AlkB

    Plasmid

    #228218

    Purpose

    Express wild type AlkB protein in E. coli BL21(DE3)

    Depositor

    Junchao Shi

    Article

    Shi et al Nat Cell Biol. 2021 Apr;23(4):424-436. d

    Insert

    Alpha-ketoglutarate-dependent Dioxygenase (alkB Synthetic)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    codon optimized

    Promoter

    T7

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. NylB'_pET21b

    Plasmid

    #215416

    Purpose

    Expression construct for NylB' gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylB'
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET20b-mPTER

    Plasmid

    #222189

    Purpose

    Bacterial expression of mouse PTER protein

    Depositor

    Jonathan Long

    Article

    Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

    Insert

    PTER (Pter Mouse)
    

    Use

    Tags

    6xHis and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET28-3'TwStrep

    Plasmid

    #208053

    Purpose

    Empty vector for prokaryotic expression, insert a Twin Strep tag (with TAA) between HindII and XhoI site of pET28a (+), both HindIII and XhoI are retained. HindIII in-frame with Twin Strep tag.

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Use

    Tags

    Twin Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET151-GyrA

    Plasmid

    #216229

    Purpose

    S. aureus USA300 GyrA Codon-optimized for E. coli

    Depositor

    Eric Brown

    Article

    French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

    Insert

    DNA gyrase
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET32a-hEGF

    Plasmid

    #199231

    Purpose

    Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

    Depositor

    Nilson Zanchin

    Article

    Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

    Insert

    Epidermal growth factor, partial (EGF Human)
    

    Use

    Tags

    TrxA-6xHis-S-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET23b-NTF2

    Plasmid

    #108918

    Purpose

    Expresses NTF2

    Depositor

    Larry Gerace

    Article

    Cassany et al Methods Mol Biol. 2009;464:181-205. doi:

    Insert

    NTF2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET28-5'TwStrep

    Plasmid

    #208620

    Purpose

    Empty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Use

    Tags

    Twin Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge