We narrowed to 125,708 results for: CIL

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pU6-BbsI-chiRNA

Plasmid

#45946

Purpose

Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.

Depositor

Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

Article

Gratz et al Genetics. 2013 May 24.

Insert

U6-BbsI-chiRNA

Use

CRISPR

Expression

Insect

Promoter

Dm-snRNA:U6:96Ab

Available Since

June 24, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2-nCas9n

Plasmid

#47929

Purpose

expression of an optimized Cas9 for genome-editing in zebrafish

Depositor

Wenbiao Chen

Article

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

Insert

nls-zcas9-nls

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pLIX_403

Plasmid

#41395

Purpose

Inducible lentiviral expression, TRE-gateway-V5; PGK-puro-2A-rtTA

Depositor

David Root

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Gateway destination vector, doxycycli…

Tags

V5

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pAdEasy-1

Plasmid

#16400

Purpose

Adenoviral vector for use in AdEasy System.

Depositor

Bert Vogelstein

Article

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Adenoviral

Expression

Mammalian

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

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FUdeltaGW-rtTA

Plasmid

#19780

Purpose

3rd generation lentiviral vector

Depositor

Konrad Hochedlinger

Article

Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

Insert

reverse tetracycline transactivator

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2009

Availability

Academic Institutions and Nonprofits only

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GW1-PercevalHR

Plasmid

#49082

Purpose

Mammalian expression vector (CMV promoter) of PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

Depositor

Gary Yellen

Article

Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

Insert

PercevalHR

Expression

Mammalian

Available Since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

Plasmid

#60910

Purpose

dCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag system

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

2x NLS, HA, and NLS

Expression

Mammalian

Promoter

dSV40 Promoter

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-GFP-Nanobody

Plasmid

#61838

Purpose

Expresses GST-tagged anti-GFP Nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

GFP-Nanobody

Tags

GST

Expression

Bacterial

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti CMV rtTA3 Hygro (w785-1)

Plasmid

#26730

Purpose

3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Hygro

Depositor

Eric Campeau

Insert

Tetracycline repressor A3 mutant

Use

Lentiviral

Expression

Mammalian

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA flag PPAR gamma

Plasmid

#8895

Purpose

Mammalian expression of flag-tagged PPAR gamma

Depositor

Bruce Spiegelman

Article

Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-

Insert

PPAR gamma (Pparg Mouse)

Tags

flag

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE

Plasmid

#60906

Purpose

The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP and a NLS for imaging

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

scFv-GCN4

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pSin-EF2-Sox2-Pur

Plasmid

#16577

Depositor

James Thomson

Article

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens SRY (sex determining region Y)-box 2 (SOX2) (SOX2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

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lenti-EF1a-dCas9-VPR-Puro

Plasmid

#99373

Purpose

3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

Depositor

Kristen Brennand

Article

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

(Sp)dCas9-VPR-P2A-Puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-S33Y Beta-catenin

Plasmid

#19286

Purpose

Mammalian expression of human beta-catenin with Flag tag and S33Y mutation

Depositor

Eric Fearon

Article

Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

Insert

beta-catenin (CTNNB1 Human)

Tags

Flag

Expression

Mammalian

Mutation

S33Y (presumptive GSK3β phosphorylation site)

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pRSETa mEos2

Plasmid

#20341

Purpose

bacterial expression of an EosFP variant, mEos2 (a photoconvertible fluorescent protein)

Depositor

Loren Looger

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

mEos-2

Tags

His

Expression

Bacterial

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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3xHA-miniTurbo-NLS_pCDNA3

Plasmid

#107172

Purpose

expresses 3xHA-tagged miniTurbo in the mammalian nucleus

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Expression

Mammalian

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-DsRed

Plasmid

#11151

Purpose

Mammalian expression vector for expression of DsRed

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has Service

Cloning Grade DNA

Insert

DsRed2 from Discosoma sp

Expression

Mammalian

Available Since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

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TETv4

Plasmid

#167983

Purpose

Expresses TETv4 (TET1CD-XTEN80-dCas9-3xNLS-P2A-BFP) downstream of the CAG promoter

Depositor

Luke Gilbert

Article

Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

Insert

TETv4 (TET1CD-XTEN80-dCas9)

Use

CRISPR and Synthetic Biology

Tags

3xNLS, TET1 catalytic domain, and tagBFP

Expression

Mammalian

Promoter

CAG

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_ABEmax_P2A_GFP

Plasmid

#112101

Purpose

A:T-to-G:C base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

ABEmax_P2A_GFP

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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SV40 1: pBSSVD2005

Plasmid

#21826

Depositor

David Ron

Insert

SV40 Large T (SV40gp6 simian virus)

Expression

Mammalian

Mutation

likely expresses aa109-708 of the large T antigen

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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