-
Plasmid#235504Purposesingle mAID_3xHA cloning vector with Roxed Bact-BsdDepositorInsertmini-Auxin Inducible Degron
UseMouse TargetingTagsmAID, HAExpressionMutationNonePromoterbeta actinAvailable sinceMay 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pT7-35aaNSP1SA11
Plasmid#220825PurposeExpress the short Rotavirus SA11 NSP1 protein (35 amino acids)DepositorInsertRotavirus SA11 35aaNSP1
UseOtherTagsExpressionMutationHas just 35aa NSP1PromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2888
Plasmid#198146PurposeEpitope tagging of effector protein SseGDepositorInsertPsseA::sscB sseF::HA sseG::M45 (sseG Salmonella enterica serovar Typhimurium)
UseTagsM45ExpressionBacterialMutationPromoterAvailable sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
MT1K_K20T
Plasmid#105708PurposeBacterial expression plasmids for production of recombinant metallothionein 1k subisoform with Thr at positon 20DepositorInsertMetallothionein 1k
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationThis subisoform has a K20T substitutionPromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-Sumo-CfaC-MBP
Plasmid#126018PurposeBacterial expression of CfaC-MBP fusion protein (with N-terminal His6-Sumo)DepositorInsertHis6-Sumo-CfaC-MBP
UseTags6xHis-SUMO and HA-MBPExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
DsRed-CPI-17-wt
Plasmid#100784PurposeMammalian expression of Fluorescent CPI-17DepositorInsertCPI-17 (PPP1R14A Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
C51m
Plasmid#120975PurposeMoClo golden gate assembly CD part for sfYFP (superfolder YFP; variant of https://www.fpbase.org/protein/yfp/ - Please see Supplemental Documents for annotated Genbank fileDepositorInsertsfYFP (has BbsI site)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[AtPsaK]:MCS+:NosT
Plasmid#212159PurposeThis binary vector has an empty backbone where you can insert a gene into a cassette with a strong promoter (truncated 35sCAMV) and UTR (truncated Arabidopsis thaliana PsaK)DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
C20m
Plasmid#120961PurposeMoClo golden gate assembly CD part with sfYFP-ssrA (superfolder YFP with ssrA degradation tag; variant of https://www.fpbase.org/protein/yfp/)DepositorInsertsfYFP-ssrA (has BbsI site)
UseSynthetic BiologyTagsssrA degradation tagExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pCS2+ H2B-mTagBFP2
Plasmid#99267PurposeContains a fusion of human histone H2B to fluorescent protein mTagBFP2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.DepositorInsertmTagBFP2-3XHA
UseTagsH2BExpressionMammalianMutationPromoterSP6Available sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOpen-T7gene5
Plasmid#165534PurposeDNA Pol with high fidelity and rapid extension rate (useful in copying long stretches of DNA); Has strong 3'-5' exonuclease.DepositorInsertT7 DNA Polymerase (unmodified)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCW57.1_TStrp_HA_Ct
Plasmid#194066Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blastiDepositorTypeEmpty backboneUseLentiviralTagsTwin_Strep-HAExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_TStrp_HA_Nt
Plasmid#194065Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blastiDepositorTypeEmpty backboneUseLentiviralTagsHA-Twin_StrepExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ H2B-Katushka2
Plasmid#99266PurposeContains a fusion of human histone H2B to fluorescent protein Katushka2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.DepositorInsertKatushka2-3XHA
UseTagsH2BExpressionMammalianMutationPromoterSP6Available sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ H2B-mCerulean
Plasmid#99268PurposeContains a fusion of human histone H2B to fluorescent protein mCerulean in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcriptionDepositorInsertmCerulean-3XFLAG
UseTagsH2BExpressionMammalianMutationPromoterSP6Available sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
BS II KS+ loxP-GFP_ZEO_loxP
Plasmid#128064Purposehas loxp-GFP-Zeo-Loxp cassetteDepositorInsertloxp-GFP-Zeo-Loxp
UseCre/LoxTagsExpressionMutationnoPromoterAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti4/V5-DEST-P110alpha
Plasmid#40230DepositorInsertP110 alpha (PIK3CA Bovine)
UseLentiviralTagsHA tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ H2B-mRuby2
Plasmid#99270PurposeContains a fusion of human histone H2B to fluorescent protein mRuby2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.DepositorInsertmRuby2-3XHA
UseTagsH2BExpressionMammalianMutationPromoterSP6Available sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ H2B-mEmerald
Plasmid#99269PurposeContains a fusion of human histone H2B to fluorescent protein mEmerald in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.DepositorInsertmEmerald
UseTagsH2BExpressionMammalianMutationPromoterSP6Available sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
SnFR_S074
Plasmid#182811PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertS074-CholineSnFR
UseTags6xHis, HA and MycExpressionBacterialMutationPromoterT7Available sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLfa[Hsp_p-GAL4Delta-SV40;attp]fa
Plasmid#86451PurposeGal4 driver construct for misexpression in Tribolium with Tribolium basal promoter; has been used for enhancer trapping; (Shuttle plasmid)DepositorInsertHsp_p-GAL4Delta-SV40;attp
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
SnFR_S062
Plasmid#182810PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertS062-CholineSnFR
UseTags6xHis, HA and MycExpressionBacterialMutationPromoterT7Available sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHCA/GAL4(848).ER
Plasmid#108215PurposeYeast expression vector for fusion of Gal4 AA 1-848 to hormone binding domain of estrogen receptor aDepositorInsertGal4-ERalpha HBD
UseTagsExpressionYeastMutationERalpha HBD has G400V mutationPromoterADH0Available sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2/shαSMA/GFP4_Seq2/clone1
Plasmid#201402PurposeKnockdown of alpha-SMA. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertalpha-SMA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
KBB528
Plasmid#185104PurposeC-terminal truncation of Pom152 fused to GFPDepositorInsertPOM152
UseTagsGFPExpressionYeastMutationPOM152(1-???)-HA-GFPPromoterAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGG-NFIC2
Plasmid#112701PurposeExpression of NFIC2 protein and GFPDepositorInsertNuclear Factor I C2 (Nfic Mouse)
UseTagsGFP and HAExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCGN-Sos Cat
Plasmid#32917DepositorInsertSOS1 (SOS1 Human)
UseTagsHAExpressionMammalianMutationContains only aa 564-1049PromoterCMVAvailable sinceJan. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ H2B-TagRFP-T
Plasmid#99271PurposeContains a fusion of human histone H2B to fluorescent protein TagRFP-T in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.DepositorInsertTagRFP-T
UseTagsH2BExpressionMammalianMutationPromoterSP6Available sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETcon(I-LtrI)
Plasmid#41523DepositorInsertI-LtrI
UseTags3x(G4S) Linker, G3S Linker, HA Tag, and Myc TagExpressionYeastMutationPromoterGAL1Available sinceApril 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
SnFR_V.7.1.2
Plasmid#182809PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertV.7.1.2-CholineSnFR
UseTags6xHis, HA and MycExpressionBacterialMutationPromoterT7Available sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pMG005_mNeonGreen_P2A
Plasmid#237215PurposeFluorescent reporter codon optimized for Dictyostelid speciesDepositorInsertmNeonGreen-P2A
UseCloningTags3xFLAG-tag, HA-tag, SV40-NLS, and nuleoplasmin NL…ExpressionMutationPromoterAvailable sinceMay 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMG008_mCherry_P2A
Plasmid#237216PurposeFluorescent reporter codon optimized for Dictyostelid speciesDepositorInsertmCherry-P2A
UseCloningTags3xFLAG-tag, HA-tag, SV40-NLS, and nuleoplasmin NL…ExpressionMutationPromoterAvailable sinceMay 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:MCS+:NosT
Plasmid#212160PurposeThis binary vector has an empty backbone with extended multiple cloning site (MSC) where you can inserta a gene into a cassette with a strong promoter (truncated 35sCAMV) and UTR (Tomato Mosaic Virus)DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
MT1G_isoform2
Plasmid#105705PurposeBacterial expression plasmids for production of recombinant metallothionein 1g subisoform 2 with deletion of Alanine 10DepositorInsertMetallothionein 1g (MT1G Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationThis subisoform has a deletion of alanine 10PromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
C105m
Plasmid#121008PurposeMoClo golden gate assembly CD part for sfYFP-AAV (superfolder YFP with AAV degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.DepositorInsertsfYFP-AAV ssrA (has bbsI)
UseSynthetic BiologyTagsAAV degradation tag for ssrAExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C106m
Plasmid#121009PurposeMoClo golden gate assembly CD part for sfYFP-DAS (superfolder YFP with DAS degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.DepositorInsertsfYFP-DAS ssrA (has bbsI)
UseSynthetic BiologyTagsDAS degradation tag for ssrAExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
-