164,127 results

1. c-Fos

   Plasmid

   #187905

   Purpose

   Expresses c-Fos in transfected cells

   Depositor

   Jennifer Mitchell

   Article

   Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

   Insert

   cFos (FOS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-PspCas13b-GEMSgRNA2

   Plasmid

   #204768

   Purpose

   pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.

   Depositor

   Kate Meyer

   Article

   Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   guide RNA targeting GEMS m6A sensor mRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-YFP

   Plasmid

   #13033

   Purpose

   Mammalian expression vector for cloning your gene fused to YFP

   Depositor

   Doug Golenbock

   Has Service

   Cloning Grade DNA

   Insert

   Yellow fluorescent protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. T7-VEE-IRES-Puro

   Plasmid

   #58970

   Purpose

   noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Type

   Empty backbone

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   26S subgenomic promoter

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP-E-Syt2

   Plasmid

   #66831

   Purpose

   Mammalian Expression, Bacterial Expression

   Depositor

   Pietro De Camilli

   Article

   Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

   Insert

   E-Syt2 (ESYT2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. C1(1-29)-TurboID-V5_pCDNA3

   Plasmid

   #107173

   Purpose

   expresses V5-tagged TurboID on the mammalian ER membrane

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-Cas9-6xHis

   Plasmid

   #62374

   Purpose

   Expression of Cas9-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   Cas9_6xHis
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH-CMV-CD19 puro

   Plasmid

   #196634

   Purpose

   Express CD19

   Depositor

   Nicolas Manel

   Article

   Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

   Insert

   CD19 (CD19 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-Rab5DN(S34N)

   Plasmid

   #35139

   Depositor

   Sergio Grinstein

   Article

   Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

   Insert

   Rab5a (RAB5A Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Dominant Negative- S34N

   Promoter

   CMV

   Available Since

   March 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. sfGFP-C1

    Plasmid

    #54579

    Purpose

    Localization: C1 Cloning Vector, Excitation: 485, Emission: 507

    Depositor

    Geoffrey Waldo, Michael Davidson

    Article

    Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

    Type

    Empty backbone

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pCRZeroT

    Plasmid

    #120276

    Purpose

    Bifunctional vector for TA-cloning and blunt-end cloning of PCR-products using ZERO-backgound system

    Depositor

    Ken Motohashi

    Article

    Motohashi et al Sci Rep. 2019 Apr 23;9(1):6417. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. [#GS24 -LV] Syn1-GFP-OMP25

    Plasmid

    #233566

    Purpose

    Lentiviral construct of mitochondrial localized GFP to be expressed in the donor cells under the Synapsin1 promotor

    Depositor

    Simon Grelet

    Article

    Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

    Insert

    eGFP (eGFP Aequorea victoria, Synthetic)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK-ATF4

    Plasmid

    #26114

    Depositor

    Yihong Ye

    Article

    Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

    Insert

    ATF4 (ATF4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-polyQ74-NLS

    Plasmid

    #231892

    Purpose

    Forms nuclear HTT partial exon 1 Q74-NLS aggregates

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    HTT partial exon 1 Q74-NLS (HTT Human)
    

    Use

    Tags

    EGFP and SV40 NLS

    Expression

    Mammalian

    Mutation

    CAG repeat expansion

    Promoter

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. ANO6-Plvx-mCherry-c1

    Plasmid

    #62554

    Purpose

    Full length mouse ANO6 tagged with mCherry on the C-terminus

    Depositor

    Renzhi Han

    Article

    Zhao et al PLoS One. 2014 Mar 24;9(3):e92749. doi:

    Insert

    mouse ANO6 (Ano6 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    March 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDestTol2pA2-9xGRE

    Plasmid

    #74736

    Purpose

    GRE-dependent EGFP reporter construct flanked by the minimal Tol2 transposon elements

    Depositor

    Luisa Dalla Valle

    Article

    Benato et al Mol Cell Endocrinol. 2014 Jul 5;392(1-2)

    Insert

    EGFP
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    nine GC-responsive elements (9xGRE) from the rat …

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-HaloAKAR2.2-T2A-EGFP

    Plasmid

    #216490

    Purpose

    Expression of chemigenetic PKA biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloAKAR2.2
    

    Use

    Tags

    T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-TRE-DIO-eGFP-2A-TetanusToxin

    Plasmid

    #166603

    Purpose

    Encodes Cre-dependent eGFP-2A tetanus toxin light chain fragment under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    EGFP-2A-Tetanus Toxin
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    tetracycline response element

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-N-Flag-Caspase-1

    Plasmid

    #75128

    Purpose

    Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    caspase 1 (Casp1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    P341S in Caspase-1 (please see depositor comments…

    Promoter

    CMV

    Available Since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. mAzurite-16xRhoBAST

    Plasmid

    #196347

    Purpose

    Expression of mAzurite mRNA with 16xRhoBAST in 3'UTR

    Depositor

    Murat Sunbul

    Article

    Englert et al Nat Commun. 2023 Jun 30;14(1):3879. doi:

    Insert

    mAzurite-16xRhoBAST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pZR071_SFFV-dCas9-mCherry-KRAB

    Plasmid

    #180264

    Purpose

    Lentiviral expression vector for dCas9-KRAB in mammalian cells

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    KRAB and mCherry

    Expression

    Mammalian

    Mutation

    Nuclease dead Cas9

    Promoter

    SFFV

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pFREE

    Plasmid

    #92050

    Purpose

    Allows inducible expression of gRNAs and Cas9 for plasmid curing.

    Depositor

    Morten Norholm

    Article

    Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

    Inserts

    Cas9
    gRNA array
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pRhamBAD and ptet

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. PIK3CA-WT

    Plasmid

    #16643

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Science. 2004 Apr 23. 304(5670):554.

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcsII-pb5-flag

    Plasmid

    #86769

    Purpose

    C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

    Depositor

    Do-Hyung Kim

    Article

    Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

    Insert

    proteasome beta subunit 5 (PSMB5 Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Cas9-GFP_sg_mAMPKa1

    Plasmid

    #79004

    Purpose

    Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    Prkaa1 (Prkaa1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

26. pXPR_011

    Plasmid

    #59702

    Purpose

    This lentiviral vector can be used to assay Cas9 activity.

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2014 Sep 3. doi: 10.1038

    Insert

    EGFP sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MYC_pLX307

    Plasmid

    #98363

    Purpose

    Lentiviral expression of MYC

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    NA

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGL3-Sox2

    Plasmid

    #101761

    Purpose

    Sox2 promoter reporter

    Depositor

    Yuh-Shan Jou

    Article

    Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

    Insert

    SRY-box 2 promoter (SOX2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pU6d:sgRNA#5

    Plasmid

    #64249

    Purpose

    expresses sgRNA under U6d promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA6-ARID1A

    Plasmid

    #39311

    Depositor

    Ie-Ming Shih

    Article

    Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

    Insert

    ARID1A (ARID1A Human)
    

    Use

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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