We narrowed to 17,163 results for: Emb;
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pMaCTag-P18
Plasmid#120029PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKeima8.5DepositorInsertmKeima8.5
UsePcr templateTagsmKeima8.5PromoterNoneAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
BcLOV-mCh-SOS
Plasmid#208279PurposeExpresses BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.DepositorInsertBcLOV-mCh-SOS
UseLentiviralPromoterSFFVAvailable SinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P10
Plasmid#120021PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKOkDepositorInsertmKOk
UsePcr templateTagsmKOkPromoterNoneAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNS3
Plasmid#131770PurposePADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sitesDepositorInsertPADH1_FUSN_Fusion Red_Cry2Olig_TACT1
UseIntegration into δ-sitesExpressionYeastMutationE490G to make Cry2 into Cry2OligPromoterPADH1Available SinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mWasabi-ER
Plasmid#164165PurposeExpresses mWasabi fused with an ER-targeting sequence of calreticulin and the ER retrieval sequence, KDEL, under the control of CAG promoterDepositorInsertmWasabi
ExpressionMammalianPromoterCAGAvailable SinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Split-Broccoli-Toehold-Switch
Plasmid#87316PurposeExpresses the Split-Broccoli system and a Trigger/Toehold switch for mCherry.DepositorInsertSplit-Broccoli system and RNA Trigger (TS2_AT01) Toehold (TS2_KS01) switch for mCherry
UseSynthetic BiologyExpressionBacterialPromoterP70aAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
TOPO CCSP
Plasmid#68453PurposeTOPO construct expressing CCSP for generation of GMAP-compatible promoterDepositorInsertClara Cell Secretory Protein Promoter
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNS1
Plasmid#131769PurposePADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sitesDepositorInsertFUSN_Fusion Red_Cry2WT
UseIntegration into δ-sitesExpressionYeastPromoterPADH1Available SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mClover3
Plasmid#171497PurposeT7 promotor drives in vitro transcription of mClover3 mRNADepositorInsertmClover3
UsePgehmePromoterT7Available SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
CBla_2.0_EmrE_TMD4_3P
Plasmid#207848PurposeBLaTM-System; Antiparallel positive control EmrE_TMD4_3P in CBLa 2.0 vectorDepositorInsertC-BLa 2.0 fusion protein
TagsFlag (DYKDHDG)ExpressionBacterialAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-basic-Polr2a promoter_600bps
Plasmid#21111DepositorInsertPolr2a promoter_600bps (POLR2A Human)
ExpressionMammalianAvailable SinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
TOPO SV40
Plasmid#68455PurposeTOPO construct expressing SV40 for generation of GMAP-compatible promoterDepositorInsertSimian Virus Early 40 Promoter
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMS33: pTwinStrep-SUMO-PmcTnsD
Plasmid#168166PurposePmcTnsD expression construct for protein purification.DepositorInsertPmcCAST TnsD
Tags6X His, Twin Strep and SUMOExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pFP.R400
Plasmid#138175PurposeExpresses the split mCherry reporter interrupted by the chimeric Npu/SspDnaE intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAc-sgRNA-Cas9.scr_2
Plasmid#190704PurposeExpresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cellsDepositorInsertScrambled sgRNA 2
UseCRISPRExpressionInsectPromoterDrosophila U6Available SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
TRF2-HA
Plasmid#200209PurposeExpression of full length mTRF2DepositorAvailable SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Zfp36-3'UTR
Plasmid#61780PurposeLuciferase reporter containing the 3'UTR of mouse Zfp36DepositorInsertmouse Zfp36 3'UTR
UseLuciferaseAvailable SinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
p5xFbx15 (-544/-527)-TK-Luc
Plasmid#15686DepositorInsert5 x Fbx15 enhancer (Oct/Sox binding site)
UseLuciferaseMutationWild-typeAvailable SinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pOTF3-AK (clone3)
Plasmid#21165DepositorInsertPOUF5F1 (genomic DNA) (POU5F1 Human)
UseCre/Lox; Homologous recombinationMutationNeoR, EGFP, and SV40 polyA are between the two ge…Available SinceJune 25, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBS/KS-mId2sp
Plasmid#16044DepositorAvailable SinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pR540
Plasmid#207885Purposetemplate for 2 gRNA amplificationDepositorInsertscaffold-pU6
ExpressionBacterialAvailable SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS021
Plasmid#207917PurposeLevel 0 for gRNA in E2 module (5' CCAT-3' ACTA)DepositorInsertpU6-AarI-scaffold-term
UseCRISPRAvailable SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKE013
Plasmid#207916PurposeLevel 0 for gRNA in E1 module (5' CTGC-3' CCAT)DepositorInsertpU3-AarI-scaffold-term
UseCRISPRAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P17
Plasmid#120028PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mEos4bDepositorInsertmEos4b
UsePcr templateTagsmEos4bPromoterNoneAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z06
Plasmid#120049PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with sfGFPDepositorInsertsfGFP
UsePcr templateTagssfGFPPromoterNoneAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA1 P186C
Plasmid#66173PurposeExpresses the P186C mutant of Sulfolobus solfataricus PCNA1DepositorInsertDNA polymerase sliding clamp 1
TagsHis6 tagExpressionBacterialMutationP186CPromoterT7 promoterAvailable SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
C514-E47: attB5r-Halotag-nostop-attB1r
Plasmid#162963PurposeGateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteinsDepositorInsertHalotag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-INPP5J
Plasmid#171949PurposeExpresses INPP5J in mammalian cellsDepositorAvailable SinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
mRuby2-AKTIP
Plasmid#171942PurposeExpresses AKTIP in mammalian cellsDepositorAvailable SinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTPI1-EMC1-GFP-APEX2-URA3
Plasmid#184776PurposeExpresses EMC1-GFP-APEX2 chimera for labeling yeast endoplasmic reticulum (ER). Uses auxotrophic marker URA3(Kluyveromyces lactis).DepositorInsertAPEX2
ExpressionYeastAvailable SinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCG-D-mSpyCatcher-His6-E
Plasmid#87376PurposeAn ORF part-containing vector harbouring the minimal SpyCatcher (ΔN1ΔC22) sequence with a His6 tag at its C-terminus and 4 bp overhangs specific for the ‘downstream SpyPart’ position within an ORF assDepositorInsertmSpyCatcher
UseSynthetic BiologyTagsHis6 tagAvailable SinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAL725
Plasmid#119862Purposeprecusor D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287CDepositorInsertD-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C (mglB Escherichia coli str. K-12)
TagsnoneExpressionBacterialMutationLeucine 244 to Cysteine, Aspartate 287 to CysteinePromoterT5Available SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSopB2xHA
Plasmid#112513PurposePsopB driven sopB-2xHA and sigEDepositorInsertsopB-2xHA/sigE operon
Tags2xHAExpressionBacterialMutationSopB C-terminal 2xHAPromoterPsopBAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMB-TGN61r
Plasmid#61175PurposeFluorescent reporter for trans-Golgi network mCherry-MtSYP61 expressed under MtBCP1 promoterDepositorInsertMtSYP61
TagsmCherryExpressionPlantPromoterMtBCP1Available SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pME18S-3HA-mULK2
Plasmid#22898DepositorAvailable SinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1-9A
Plasmid#114023PurposeInducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell linesDepositorAvailable SinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMC-Ura-Cen-lvl1
Plasmid#176182Purposepreassembled yeast MoClo level-1 backbone with GFP dropout and URA3 markerDepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
p1DT (chicken beta 1 integrin)
Plasmid#14066DepositorInsertChicken Beta 1 Integrin (ITGB1 Chicken)
ExpressionMammalianAvailable SinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
Lyn-FRB-GFP11
Plasmid#181863PurposePlasma membrane-localized GFP11; for plasma membrane targeting of FluoSTEPs.DepositorInsertLyn-FRB-GFP11
TagsGFP11 and N-terminal targeting sequence from Lyn …ExpressionMammalianPromoterCMVAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
Dppa1-T2A-H2B-Halo donor
Plasmid#113118PurposeDonor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Dppa1 coding sequenceDepositorInsertDppa1 donor region including homology arms and T2A-H2B-Halo
UseSynthetic BiologyAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Rb WT
Plasmid#32390DepositorAvailable SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
KG#59
Plasmid#110929PurposeExpresses proteins pan-neuronally in C. elegans (entire nervous system)DepositorInsertsrab-3 promoter
unc-54 3' control region
ExpressionBacterialAvailable SinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pQE60 Trx1 SSAAA
Plasmid#98403PurposeBacterial expression vector for Trx1 SSAAADepositorAvailable SinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
XE7 Xwnt-8
Plasmid#16869DepositorInsertXwnt-8 RNA (wnt8a.L Frog)
UseXenopus expressionAvailable SinceMarch 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P01
Plasmid#120012PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxBFPDepositorInsertmoxBFP
UsePcr templateTagsmoxBFPPromoterNoneAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBGSA FPR1
Plasmid#62600PurposeConstitutive expression of FPR1 in mammalian cellsDepositorAvailable SinceFeb. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
iRFP713-FRB-PIP5K1A
Plasmid#202743Purposeexpresses recruitable fluorescent protein-tagged enzymeDepositorInsertiRFP713:MTOR(2021-2113):GGSA2:PIP5K1A (PIP5K1A Human)
ExpressionMammalianAvailable SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only
