163,606 results

1. pET-Cas9-6xHis

   Plasmid

   #62374

   Purpose

   Expression of Cas9-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   Cas9_6xHis
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV6M-Pak1 K299R

   Plasmid

   #12210

   Depositor

   Jonathan Chernoff

   Article

   Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

   Insert

   Pak1 (PAK1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Catalytically inactive Pak1: K299R

   Promoter

   Available Since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG1344 EGFP-FIREtag

   Plasmid

   #205165

   Purpose

   Expresses EGFP-FIREtag in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   EGFP-FIREtag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCdrA-gfpC

   Plasmid

   #111614

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(Mut3)-T0-T1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV_TRET_hNgn2_UBC_Puro

   Plasmid

   #61474

   Purpose

   3rd generation lentiviral vector; TetON promoter driving human Ngn2; constitutive expression of Puromycin selection marker

   Depositor

   Ron Weiss

   Article

   Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

   Inserts

   Ngn2 (NEUROG2 Human)
   Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. nuclear cpVenus control

   Plasmid

   #186790

   Purpose

   Identical to nuclear NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

   Depositor

   Michael Cohen

   Article

   Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

   Insert

   nuclear cpVenus control
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1_AGIA-AirID

   Plasmid

   #182210

   Purpose

   Express AirID in mammalian cells.

   Depositor

   Akira Nozawa, Sohei Ito, Shogo Nakano, Tatsuya Sawasaki

   Article

   Kido et al Elife. 2020 May 11;9. pii: 54983. doi: 1

   Insert

   AGIA-AirID
   

   Use

   Tags

   AGIA-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-HA-Tuba

   Plasmid

   #22214

   Depositor

   Pietro De Camilli

   Article

   Salazar et al J Biol Chem. 2003 Dec 5. 278(49):49031-4

   Insert

   Tuba (DNMBP Human)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pRC0940 (circRNA entry vector)

   Plasmid

   #189967

   Purpose

   Cloning vector for generating template plasmids to transcribe circRNA, with mNeonGreen dropout following BsaI digestion

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHR- flag-Lag16-JAM-B

    Plasmid

    #205200

    Purpose

    This vector encodes of the synCAM (JAM-B) and GFP nanobody LAG16

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM JAM-B and Lag16 anti-GFP nanobody
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pOsU3

    Plasmid

    #170132

    Purpose

    For sgRNA expression in monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV SYN flex PSAM4 GlyR IRES EGFP

    Plasmid

    #119741

    Purpose

    Cre-dependent chemogenetic inhibitor expression

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Has Service

    AAV5 and AAV9

    Insert

    PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F

    Promoter

    synapsin

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Sumo3 PolQM1

    Plasmid

    #78462

    Purpose

    Inducible expression in E. coli

    Depositor

    Susan Wallace, Sylvie Doublie

    Article

    Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

    Insert

    Polymerase theta (POLQ Human)
    

    Use

    Tags

    His and SUMO3

    Expression

    Bacterial

    Mutation

    delta 1-1791; Q2513R (please see depositor commen…

    Promoter

    T7

    Available Since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

14. c-Fos

    Plasmid

    #187905

    Purpose

    Expresses c-Fos in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

    Insert

    cFos (FOS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMi(3xP3-EGFP)

    Plasmid

    #102540

    Purpose

    Transgenesis vector based on the Minos transposon, carrying the 3xP3-EGFP marker

    Depositor

    Charalambos Savakis, Michalis Averof

    Article

    Pavlopoulos et al Genetics. 2004 Jun;167(2):737-46.

    Insert

    Minos transpoable element with 3xP3-EGFP marker
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. PJEBAN6

    Plasmid

    #203684

    Purpose

    Expresses red fluorescent marker DsRed-Express in Listeria monocytogenes

    Depositor

    Tine Rask Licht

    Article

    Andersen et al BMC Microbiol. 2006 Oct 3;6:86. doi: 10.

    Insert

    DsRed-Express
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-iCre

    Plasmid

    #89573

    Purpose

    Expresses iCre under pCAG

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    iCre
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSL3617 (pCRISPR(BbsI)_Pse)

    Plasmid

    #200902

    Purpose

    Expresses Pseudoalteromonas CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    PseCRISPR (entry)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry

    Plasmid

    #75470

    Purpose

    Flp-dependent Chr2-mCherry encoded in an AAV vector for rAAV production and expression in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

    Has Service

    AAV1

    Insert

    Reversed ChR2(H134R)-mCherry
    

    Use

    AAV and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCIP-AMPKa1_WT

    Plasmid

    #79010

    Purpose

    For mammalian expression of WT AMPK alpha1.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    AMPK alpha 1 (PRKAA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGs

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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