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pTn7-SCOUT12.G

Plasmid

#201000

Purpose

pLv1-pUC18R6KT-miniTn7-Km-sfGFP, KmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

superfolder GFP

Expression

Bacterial

Promoter

J23104

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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piGECInano-N1

Plasmid

#186190

Purpose

Expression of small FRET-based near-infrared genetically-encoded calcium indicator in mammalian cells

Depositor

Vladislav Verkhusha

Article

Matlashov et al Front Cell Dev Biol. 2022 Jun 29;10:8801

Insert

iGECInano

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRSETb-pcDronpa2

Plasmid

#78184

Purpose

Expresses pcDronpa2 in E. coli strains

Depositor

Peter Dedecker

Article

Moeyaert et al ACS Nano. 2014 Feb 25;8(2):1664-73. doi:

Insert

pcDronpa2

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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SS9_RNA

Plasmid

#71656

Purpose

SS9-targeting gRNA for co-transformation with pSS9

Depositor

Ryan Gill

Article

Bassalo et al ACS Synth Biol. 2016 Apr 22.

Insert

SS9 gRNA

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomKate2-CaURA3

Plasmid

#44878

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mKate2 with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomKate2

Expression

Yeast

Available Since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Beta4Gal-HaloTag7

Plasmid

#175536

Purpose

Expression of HaloTag7 at the at the Golgi apparatus of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Beta4Gal-HaloTag7

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GR-ICUE2

Plasmid

#173017

Purpose

Improved green-red FRET based cAMP indicator.

Depositor

Jin Zhang

Article

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

GR-ICUE2

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-SiT-N-15

Plasmid

#55325

Purpose

Localization: Golgi TGN, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

SiT (ST6GAL1 Human)

Tags

mTagBFP2

Expression

Mammalian

Mutation

aa 1-45 of SiT

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pUC19/N-mChe

Plasmid

#183159

Purpose

Plant transient expression vector containing mCherry tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of mCherry.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLG025 (DUF4297 + HerA)

Plasmid

#157903

Purpose

Expresses the DUF4297 + HerA defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

DUF4297 + HerA

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-ymScarletI-CaURA3

Plasmid

#118457

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized mScarletI with CaUra3 selection. *An updated version of this plasmid is available: #168055 pFA6a-link-ymScarletI-URA3.

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymScarletI

Expression

Yeast

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-ymNeongreen-CaURA3

Plasmid

#125703

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection. *An updated version of this plasmid is available: #168059 pFA6a-link-ymNeongreen-URA3.

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymNeongreen

Expression

Yeast

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-smURFP

Plasmid

#197198

Purpose

Expresses the protein of smURFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

smURFP

Use

AAV

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDIV296

Plasmid

#204687

Purpose

AMA1 plasmid with nat1 resistance marker

Depositor

Uffe Mortensen

Article

Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

Insert

nat1

Use

Fungal expression

Expression

Bacterial

Promoter

Aspergillus nidulans trpC promoter

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_HaloTag9_SKL

Plasmid

#175542

Purpose

Peroxysomal expression of HaloTag9 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag9-SKL

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pSG15-pBAD-tetO-deGFP

Plasmid

#102450

Purpose

Expresses deGFP fluorescent protein in E. coli. The expression is controlled by a pBAD-tetO combinatorial promoter.

Depositor

Richard Murray

Article

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

deGFP

Expression

Bacterial

Promoter

pBAD-tetO

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-iRFP682

Plasmid

#197204

Purpose

Expresses the protein of iRFP682 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

iRFP682

Use

AAV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pSF11-wmiRFP713-T2A-HO1

Plasmid

#197246

Purpose

Expresses the protein of wmiRFP713-T2A-HO1 in neurons of C. elegans

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmiRFP713-T2A-HO1

Expression

Worm

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pNOC-ARS-stacked-NeoR-mNeonGreen

Plasmid

#101249

Purpose

Construct for fusion expression of mNeonGreen reporter and G418 resistance gene by a bidirectional promoter

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Promoter

Ribi

Available Since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pGFP

Plasmid

#64904

Purpose

"Fluorescent reporter containing no disulfide bonds/Selenocystamine folding"

Depositor

Stephen Mayfield

Article

Ferreira-Camargo et al AMB Express. 2015 Dec;5(1):126. doi: 10.

Insert

Green fluorescent protein

Use

Psba chlamydomonas reinhardtii vector

Promoter

psbA

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only
pEB1-moxGFP

Plasmid

#103984

Purpose

Plasmid encoding moxGFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

moxGFP

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S30R, Y39N, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pAquaN1

Plasmid

#42888

Depositor

Fabienne Merola

Article

Erard et al Mol Biosyst. 2013 Feb 2;9(2):258-67. doi

Insert

Aquamarine

Expression

Mammalian

Mutation

T66S and H149G

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-mEOS3.2-KanMX6

Plasmid

#87030

Purpose

Tag S. pombe genes in C terminal with mEOS3.2

Depositor

Julien Berro, Thomas Pollard

Article

Laplante et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

Insert

mEOS3.2

Expression

Bacterial and Yeast

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-MapTau

Plasmid

#137808

Purpose

Visualization of microtubules

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

MapTau

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mRhubarb713

Plasmid

#197207

Purpose

Expresses the protein of mRhubarb713 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mRhubarb713

Use

AAV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pRYG

Plasmid

#113642

Purpose

Positive control: shows the ratio of GFP signal to RFP signal with a tyrosine residue in the linker that connects the two genes.

Depositor

Jeffrey Barrick

Article

Monk et al ACS Synth Biol. 2016 Sep 20.

Insert

mRFP1-linker-sfGFP

Use

Synthetic Biology

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaB-iRFPBb

Plasmid

#118237

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven iRFP

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven iRFP

Expression

Bacterial

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-K560-E236A-24xGCN4_v1-IRES-Puro

Plasmid

#60909

Purpose

A minimal K560 kinesin rigor motor tagged with the SunTag for single molecule imaging

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

K560-E236A

Expression

Mammalian

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pS3

Plasmid

#50596

Purpose

Backbone: pBbB5c Origin Of Replication:pBBR1 Selection Markers: Chloramphenicol Promoters: PLacUV5

Depositor

Jay Keasling

Article

Juminaga et al Appl Environ Microbiol. 2012 Jan;78(1):8

Inserts

aroE
aroD
aroB
aroG*
ppsA
tktA

Use

Synthetic Biology

Expression

Bacterial

Promoter

lacUV5

Available Since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only

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pEB1-mVenus ME

Plasmid

#103989

Purpose

Plasmid encoding mVenus ME

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mVenus ME

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F46L, F64L, …

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pKIKOrbsARCm

Plasmid

#46765

Purpose

E.coli genomic integration rbsAR locus

Depositor

Lars Nielsen, Claudia Vickers

Article

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pET28a-eGFP-MinD

Plasmid

#133622

Purpose

Expression vector for the Escherichia coli MinD with an N-terminal fusion of a His-tag for purification and an EGFP for visualization.

Depositor

Petra Schwille

Article

Zieske et al FEBS Lett. 2014 Aug 1;588(15):2545-9. do

Insert

EGFP-MinD (minD E. coli)

Tags

His

Expression

Bacterial

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLG028 (hhe)

Plasmid

#157906

Purpose

Expresses the hhe defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

hhe (DUF4011-helicase-Vsr-DUF3320)

Expression

Bacterial

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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WT-Pan

Plasmid

#99638

Purpose

EGFP-mCherry control reporter for methionine misincorporation

Depositor

Tao Pan

Article

Gomes et al RNA. 2016 Mar;22(3):467-76. doi: 10.1261

Insert

mCherry

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pmEGFP-ADRA2A

Plasmid

#190753

Purpose

Expression of ADRA2A with mEGFP tag on N-terminus.

Depositor

Dave Piston

Article

Foust et al bioRxiv 2021.11.03.467169

Insert

ADRA2A (ADRA2A Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLG032 (ppl)

Plasmid

#157910

Purpose

Expresses the ppl defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

ppl (PHP-ATPase)

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pKIKOrbsARKm

Plasmid

#46768

Purpose

E.coli genomic integration rbsAR locus

Depositor

Lars Nielsen, Claudia Vickers

Article

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Available Since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pBad-UGAcS

Plasmid

#172137

Purpose

bacteria expression plasmid for a UDP-GlcNAc sensor

Depositor

Huiwang Ai

Article

Li et al ACS Cent Sci. 2021 Oct 27;7(10):1763-177

Insert

UGAcS

Expression

Bacterial

Mutation

H605Q- please see depositor comments

Promoter

araBAD

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pFPL-Rh

Plasmid

#61649

Purpose

Gateway vector for generating red fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

monomeric red fluorescent protein

Expression

Plant

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb

Plasmid

#175534

Purpose

Expression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

IgKchL_HA-HaloTag7-myc_PDGFRtmb

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMH062

Plasmid

#225537

Purpose

Expresses IS621 in insect cells

Depositor

Hiroshi Nishimasu

Article

Hiraizumi et al Nature. 2024 Jun;630(8018):994-1002. doi

Insert

IS621

Expression

Insect

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ERoxBFP

Plasmid

#68126

Purpose

mammalian expression of ER localized oxBFP

Depositor

Erik Snapp

Article

Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

Insert

oxBFP

Tags

KDEL ER retention sequence and bovine prolactin s…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only
pScI_dCas9-CDA

Plasmid

#108549

Purpose

Bacterial Target-AID vector

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Insert

dCas9-PmCDA1

Tags

Flag

Expression

Bacterial

Mutation

D10A and H840A for SpCas9

Promoter

lambda OR

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yomCherry-CaURA3

Plasmid

#44876

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mCherry with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomCherry

Expression

Yeast

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
T444T-GFP(NCO)

Plasmid

#183972

Purpose

Enhanced RNAi clone for C. elegans knockdown of non-codon-optimized GFP

Depositor

David Matus

Article

Palmisano et al CourseSource, 2022

Insert

GFP (non-codon-optimized)

Use

RNAi

Expression

Worm

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLENTI_RPS10_K139R

Plasmid

#127137

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS10 (RPS10 Human)

Use

Lentiviral

Mutation

K139R

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLENTI_RPS10_K138R

Plasmid

#127136

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS10 (RPS10 Human)

Use

Lentiviral

Mutation

K138R

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pNIR-Fb59H10

Plasmid

#184682

Purpose

NIR-Fb to HIV p24

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-Fb59H10

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yomEos2-Kan

Plasmid

#44944

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mEos2 with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomEos2

Expression

Yeast

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRBAD-link-CGFP

Plasmid

#52733

Purpose

L-(+)-arabinose-inducible expression of link-CGFP negative control for in vivo split GFP assembly assay

Depositor

Lynne Regan

Article

Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

Insert

link-CGFP

Expression

Bacterial

Promoter

PBAD

Available Since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 22,610 results for: ESC