8,883 results

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pcDNA3.1 Hmga2 wt

Plasmid

#14789

Depositor

David Bartel

Article

Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

Insert

Hmga2 wt (Hmga2 Mouse)

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

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hTLR5 flag

Plasmid

#13088

Depositor

Ruslan Medzhitov

Insert

TLR5 (TLR5 Human)

Tags

Flag

Expression

Mammalian

Mutation

Amino acids 1-19 have been replaced by a mammalia…

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pCerulean-VSVG

Plasmid

#11913

Depositor

Jennifer Lippincott-Schwartz

Article

Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

Insert

VSVG

Expression

Mammalian

Mutation

F64L, S65T, Y66W, S72A, N146I, Y145A, H148D, M15…

Available Since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagB wt

Plasmid

#19301

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding B (RRAGB Human)

Tags

GST and HA

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pZW16 (CK2alpha')

Plasmid

#27087

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha' (CSNK2A2 Human)

Tags

HA

Expression

Mammalian

Available Since

June 21, 2012

Availability

Academic Institutions and Nonprofits only

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pST1374-N-NLS-flag-linker-Cas9-D10A

Plasmid

#51130

Purpose

Cas9 nickase optimized for nuclear import.

Depositor

Xingxu Huang

Article

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Insert

Cas9 nickase

Use

CRISPR

Tags

Flag and NLS

Expression

Mammalian

Mutation

D10A (nickase mutant)

Promoter

CMV

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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NGFR P210

Plasmid

#27486

Depositor

Warren Pear

Article

He et al Blood. 2002 Apr 15;99(8):2957-68.

Insert

bcr/abl (P210) (ABL1, BCR Human)

Use

Retroviral

Tags

IRES and NFGR

Expression

Mammalian

Mutation

Contains the "complete" bcr/abl fusion

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA cyclin D1 HA

Plasmid

#11181

Depositor

Bruce Zetter

Article

Newman et al J Biol Chem. 2004 Oct 1. 279(40):41504-1

Insert

cyclin D1 (CCND1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
PBX1A-pCMV1

Plasmid

#21029

Depositor

Corey Largman

Insert

PBX1 (PBX1 Human)

Expression

Mammalian

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
Tet-O-FUW-Sox10

Plasmid

#45843

Depositor

Marius Wernig

Article

Yang et al Nat Biotechnol. 2013 May;31(5):434-9. do

Insert

Sox10 (Sox10 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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YFP-FKBP (YF)

Plasmid

#20175

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FKBP (YF): (FKBP1A Human)

Expression

Mammalian

Mutation

FKBP was taken out from pC4M-F2E with a flanking …

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV5 mouse src

Plasmid

#13663

Depositor

Joan Brugge, Peter Howley

Insert

src (Src Mouse)

Tags

Myr

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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hTLR3 flag

Plasmid

#13084

Depositor

Ruslan Medzhitov

Insert

TLR3 (TLR3 Human)

Tags

Flag

Expression

Mammalian

Mutation

Amino acids 1-17 have been replaced by mammalian …

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
Mito-ABKAR

Plasmid

#61509

Purpose

Expression of AMPK biosensor targeted to the mitochondria

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Tags

Mitochondrial targeting signal (DAKAP1-30)

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
aMHC-eGFP-Rex-Neo

Plasmid

#21229

Depositor

Mark Mercola

Article

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

alpha myosin heavy chain promoter

Use

Lentiviral

Tags

eGFP

Available Since

June 30, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-fSST-RFP

Plasmid

#22913

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

fugu somatostatin promoter driving DsRed 2

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-FLT1

Plasmid

#23912

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FLT1 (FLT1 Human)

Use

Gateway donor vector

Mutation

S755P

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pY30

Plasmid

#84745

Purpose

Expresses huAsCpf1-P2A-puro and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huAsCpf1
puromycin resistance gene

Tags

3xHA, NLS, and T2A

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pC.HA Vav1

Plasmid

#14553

Depositor

Joan Brugge

Article

Moores et al Mol Cell Biol. 2000 Sep . 20(17):6364-73

Insert

Vav1 (VAV1 Human)

Tags

HA

Expression

Mammalian

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
PL-SIN-EOS-C(3+)-EGFP

Plasmid

#21318

Purpose

Lentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Expression

Mammalian

Promoter

EOS-C(3+) promoter region

Available Since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
gRNA_GFP-T2

Plasmid

#41820

Purpose

Expresses a guide RNA (gRNA) to target GFP (T2 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_GFP-T2

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-HA GST RagA wt

Plasmid

#19298

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding A (RRAGA Human)

Tags

GST and HA

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pBRIT Pax7 FLAG

Plasmid

#17521

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pEN84 - CTCF-AID[71-114]-eGFP-FRT-Puro-FRT targeting construct

Plasmid

#86230

Purpose

Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using puromycine selection. Auxin-inducible degron system.

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

AID[71-114]-eGFP

Use

Mouse Targeting

Expression

Mammalian

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 mHIF-2α MYC (P405A/P530V/N851A)

Plasmid

#44027

Depositor

Celeste Simon

Article

Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

Insert

mHIF-2α (P405A/P530V/N851A) (Epas1 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

P405A, P530V, N851A

Promoter

CMV

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 deltaN47 Beta-catenin

Plasmid

#19287

Depositor

Eric Fearon

Article

Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

Insert

beta-catenin (CTNNB1 Human)

Tags

Flag

Expression

Mammalian

Mutation

N-terminal truncation, deleted aa 1-47

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 SARS-CoV-2 NSP15

Plasmid

#141268

Purpose

Gateway-compatible Entry vector, with insert of NSP15 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP15 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHA-PUMA

Plasmid

#16588

Depositor

Bert Vogelstein

Article

Yu et al Mol Cell. 2001 Mar . 7(3):673-82.

Insert

PUMA (BBC3 Human)

Tags

2x HA

Expression

Mammalian

Available Since

April 24, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
mEGFP-HRas S17N

Plasmid

#18665

Depositor

Karel Svoboda

Article

Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

Insert

H-Ras (HRAS Human)

Tags

monomeric EGFP

Expression

Mammalian

Mutation

S17N mutation in H-Ras

Available Since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
E[beta]P

Plasmid

#24313

Depositor

Roel Nusse

Article

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

EF1alpha-betacatenin(deltaGSK) // SV40-PuroR (Ctnnb1 Mouse)

Use

Lentiviral

Mutation

Sites phosphorylated by GSK-3beta have been mutat…

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yomKate2-CaURA3

Plasmid

#44878

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mKate2 with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomKate2

Expression

Yeast

Available Since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pTLR repair vector

Plasmid

#64322

Purpose

Vector for repair of the traffic light reporter (TLR), providing the venus coding region

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Insert

Venus

Use

Artificial targeting vector

Mutation

see publication for details

Promoter

none

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
SS9_RNA

Plasmid

#71656

Purpose

SS9-targeting gRNA for co-transformation with pSS9

Depositor

Ryan Gill

Article

Bassalo et al ACS Synth Biol. 2016 Apr 22.

Insert

SS9 gRNA

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-Farnesyl-5

Plasmid

#55045

Purpose

Localization: Plasma Membrane, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Farnesyl (HRAS Human)

Tags

mCherry

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL Neo Myr Flag HK2

Plasmid

#20501

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

HK2 (HK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3 Flag p38 beta2

Plasmid

#20355

Depositor

Roger Davis

Article

Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

Insert

pDCNA3 Flag p38 beta2 (MAPK11 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-HA-USP42

Plasmid

#22603

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP42 (USP42 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
Ub-R-YFP

Plasmid

#11948

Depositor

Nico Dantuma

Article

Menendez-Benito et al Hum Mol Genet. 2005 Oct 1. 14(19):2787-9

Insert

Ub-R-YFP

Expression

Mammalian

Mutation

Ubiquitin fused to N-terminus of YFP. Arginine at…

Available Since

July 5, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-rab9 DN

Plasmid

#12664

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB9 (Homo sapiens) (RAB9A Human)

Tags

GFP and HA

Expression

Mammalian

Mutation

changed serine 21 to asparagine

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG.PKCzeta

Plasmid

#10799

Depositor

Alex Toker

Article

Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

Insert

PKC zeta (Prkcz Rat)

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pRK5-TGF beta Type II receptor Flag

Plasmid

#31719

Depositor

Rik Derynck

Insert

TGF beta Type II Receptor (TGFBR2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only
pDONR223-KDR

Plasmid

#23925

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

KDR (KDR Human)

Use

Gateway donor vector

Mutation

V297I

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
mKO2-C1

Plasmid

#54494

Purpose

Localization: C1 Cloning Vector, Excitation: 551, Emission: 565

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Sakaue-Sawano et al Cell. 2008 Feb 8;132(3):487-98. doi: 10.

Type

Empty backbone

Tags

mKO2

Expression

Mammalian

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
PX851

Plasmid

#62883

Purpose

N-term SpCas9 piece of inducible split-4 (Cas9(N)-FRB-NES split-4).

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa 2-535)

Use

CRISPR

Tags

FRB and NES PTK2

Expression

Mammalian

Promoter

CBh

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
PX852

Plasmid

#62884

Purpose

C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa536-1368)

Use

CRISPR

Tags

FKBP12, NLS SV40, and NLS nucleoplasmin

Expression

Mammalian

Promoter

CBh

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMGS54 (AID-P2A-Hygromycin)

Plasmid

#126583

Purpose

An AID-P2A-HygR cassette to tag the protein of interests with auxin-inducible degron at the c-terminal

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

AID-P2A-HygR

Use

Other

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFNF

Plasmid

#22687

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

Neo*

Use

Mouse Targeting

Expression

Bacterial and Mammalian

Mutation

FRT-pPGK-pEM7-Neo*-bpA-FRT

Available Since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-MAP3K7

Plasmid

#23693

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP3K7 (MAP3K7 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
Pin1

Plasmid

#40773

Depositor

Dustin Maly

Insert

Pin1 (PIN1 Human)

Tags

6His and TEV

Expression

Bacterial

Mutation

Starting Met removed

Promoter

T7

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH376

Plasmid

#86842

Purpose

mamalian expression of anti-CRISPR protein AcrIIA4

Depositor

Joseph Bondy-Denomy

Article

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA4

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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8,883 results