We narrowed to 149,107 results for: ins;

1. YW249_Pzipt17-loxP-H2BGFP-miniMOS

   Plasmid

   #196063

   Purpose

   a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of SL2::H2B::GFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and GFP cassettes.

   Depositor

   Wenxing Yang

   Article

   Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

   Inserts

   H2B::GFP
   NeoR
   Minimal Mos1
   zipt17p::zipt17::zipt17 3UTR
   

   Expression

   Worm

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX SHP-1(C)-SH2

   Plasmid

   #46494

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   SHP-1(C) (PTPN6 Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 105-217

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. p-mCherry-C1-iCoChR++

   Plasmid

   #98173

   Purpose

   artificial anion conducting channelrhodopsin (aACR). Activation with blue light. Codon optimized for mammalian expression.

   Depositor

   Peter Hegemann

   Article

   Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

   Insert

   Synthetic construct iCoChR++ gene
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   A59S, E83N, E90Q, E101S, Q117R, E123S, V242R, T24…

   Promoter

   CMV (+enhancer)

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-hMb3Cas12a-NLS(nucleoplasmin)-3xHA (RTW2500)

   Plasmid

   #115142

   Purpose

   CAG promoter expression plasmid for human codon optimized Mb3Cas12a nuclease with C-terminal NLS and HA tag

   Depositor

   Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized Mb3Cas12a
   

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX Crk-SH2

   Plasmid

   #46418

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Crk (CRK Human)
   

   Tags

   AviTag, GST, and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 1-124

   Promoter

   Tac

   Available Since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. SLK_HUMAN_D0

   Plasmid

   #79733

   Purpose

   This plasmid encodes the kinase domain of SLK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   SLK (SLK Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pEMS1725

   Plasmid

   #105870

   Purpose

   High copy number plasmid containing iCreERT2-FFrt-KanR-FFrt insert

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

   Insert

   iCreERT2-FFrt-KanR-FFrt
   

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNCST-AausFP2

   Plasmid

   #129500

   Purpose

   Expresses AausFP2 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   AausFP2
   

   Expression

   Bacterial

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKK-HA-TEV

   Plasmid

   #105769

   Purpose

   Expression of your protein of interest in fusion with HA at the N-terminus. The tag is cleavable by TEV protease.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   HA-TEV

   Expression

   Mammalian

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX p85a(C)-SH2

    Plasmid

    #46463

    Depositor

    Leszek Kotula, Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    p85a(C) (PIK3R1 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 612-724

    Promoter

    Tac

    Available Since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. Pf113-bio

    Plasmid

    #47729

    Purpose

    Expresses enzymatically monobiotinylated full-length Pf113 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised Pf113
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSG5-HA-ERR3 S57,81,219A

    Plasmid

    #37850

    Depositor

    Rebecca Riggins

    Article

    Heckler et al FEBS J. 2014 Mar 29. doi: 10.1111/febs.1

    Insert

    Estrogen-related receptor gamma (Esrrg Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Serines 57, 81, and 219 to Alanine (AGC t…

    Promoter

    SV40

    Available Since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(+)

    Plasmid

    #184334

    Purpose

    Expression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.

    Depositor

    Peter Scheiffele

    Article

    Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

    Insert

    Nrxn3-alpha (Nrxn3 Mouse)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. HOXD4 (murine) MSCV

    Plasmid

    #8549

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXD4 (Hoxd4 Mouse)
    

    Use

    Retroviral

    Tags

    His and T7

    Expression

    Mammalian

    Mutation

    contains T7 and HIS tags from pET; makes protein …

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pKK-TEV-HA

    Plasmid

    #105785

    Purpose

    Expression of your protein of interest in fusion with HA at the C-terminus. The tag is cleavable by TEV protease.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-HA

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNCST-AausGFP

    Plasmid

    #129503

    Purpose

    Expresses AausGFP constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AausGFP
    

    Expression

    Bacterial

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-CRY2–mPKCε-CAT-HA

    Plasmid

    #190483

    Purpose

    Expresses a fluorescently-tagged, optogenetically activated PKC-epsilon. Used in tandem with CIBN-CAAX

    Depositor

    Diomedes Logothetis

    Article

    Gada et al J Biol Chem. 2022 May;298(5):101893. doi

    Insert

    mCherry-CRY2–mPKCε-CAT-HA (Prkce Mouse, Synthetic)
    

    Tags

    Fused to CRY2, HA, and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-iLMO4 (GLucM23-iChloC-EYFP)

    Plasmid

    #114102

    Purpose

    fusion protein of Gaussia luciferase variant M23, improved slowChloC anion conducting ChR, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Park et al J Neurosci Res. 2017 Sep 1. doi: 10.1002

    Insert

    GLucM23-iChloC-EYFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. NFATc2-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75232

    Purpose

    CRISPR/Cas9 plasmid against human NFATc2

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc2 (NFATC2 Human)
    

    Use

    CRISPR

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pB80-iLID-mCherry-RAB11

    Plasmid

    #174623

    Purpose

    Optogenetic coupling to recycling endosomes (RAB11) via iLID

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    iLID-mCherry-RAB11 (RAB11A Human, Synthetic)
    

    Tags

    iLID-mCherry

    Expression

    Mammalian

    Mutation

    mCherry: Met1Del; RAB11: Met1Del

    Promoter

    Chicken beta-actin

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pGEX Fes-SH2

    Plasmid

    #46426

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Fes (FES Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 396-497

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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22. DICER KO vector

    Plasmid

    #16656

    Depositor

    Bert Vogelstein

    Article

    Cummins et al Proc Natl Acad Sci U S A. 2006 Mar 7. 10

    Insert

    Dicer knock-out (DICER1 Human)
    

    Use

    Adeno-associated viral

    Expression

    Mammalian

    Available Since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

23. pGEX p85a(N)-SH2

    Plasmid

    #46464

    Depositor

    Leszek Kotula, Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    p85a(N) (PIK3R1 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 321-434

    Promoter

    Tac

    Available Since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRRL.SIN.EF1A.JAG1-F1.GS.CAR-2G

    Plasmid

    #194461

    Purpose

    CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VH-VL order and (GGGGS)3 linker (scFv). GFP-Zeo for selection and monitoring.

    Depositor

    Ana Sofia Coroadinha

    Article

    Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

    Insert

    Anti-JAG1 CAR
    

    Use

    Lentiviral

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p-mCherry-C1-iC++

    Plasmid

    #98165

    Purpose

    artificial anion conducting channelrhodopsin (aACR). Activation max 488 nm; off-kinetics 35 ms. Codon optimized for mammalian expression.

    Depositor

    Peter Hegemann

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct iC++ gene
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, V242R, T24…

    Promoter

    CMV (+enhancer)

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCS2+ cMyc Dam_f_GFPoNLS

    Plasmid

    #85820

    Purpose

    iDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

    Depositor

    Joachim Wittbrodt

    Article

    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

    Insert

    cMyc Dam_f_GFPoNLS
    

    Tags

    cMyc and oNLS (optimized nuclear localization sig…

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    L122A.

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGEX SHP-1(N)-SH2

    Plasmid

    #46495

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHP-1(N) (PTPN6 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1-109

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Polymerase expression - pCMV-B103 (LM2907)

    Plasmid

    #208968

    Purpose

    Unfused B103 DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    B103-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRSET a sfMatryoshCaMP6s-T78H

    Plasmid

    #100021

    Purpose

    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78H

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    sfMatryoshCaMP6s
    

    Tags

    6x HIS tag and Xpress_EK tag

    Expression

    Bacterial

    Mutation

    cpEGFP replaced with superfolder cpGFP and T78H m…

    Promoter

    T7

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p10xUAS-IVS- Syn21-Voltron-p10

    Plasmid

    #119041

    Purpose

    UAS-driven expresion of Voltron in Drosophila

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

    Insert

    Voltron
    

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRRL.SIN.EF1A.JAG1-B5.GS.CAR-2G

    Plasmid

    #194463

    Purpose

    CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.B5 in VH-VL order and (GGGGS)3 linker (scFv). GFP-Zeo for selection and monitoring.

    Depositor

    Ana Sofia Coroadinha

    Article

    Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

    Insert

    Anti-JAG1 CAR
    

    Use

    Lentiviral

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pNCST-AausFP3

    Plasmid

    #129501

    Purpose

    Expresses AausFP3 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AausFP3
    

    Expression

    Bacterial

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Cry2 mCherry PGK-1 in pcDNA3.1

    Plasmid

    #64214

    Purpose

    mCherry fused between Cry2 and PGK-1 to study cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

    Inserts

    Cry2 PHR (CRY2 Mustard Weed)
    PGK1 (PGK1 Human)
    

    Tags

    mcherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

34. pDRF'- AmTryoshka1;3-LS-F138I-T78H

    Plasmid

    #100963

    Purpose

    Yeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    AmTryoshka1;3-LS-F138I-T78H (AMT1;3 Mustard Weed)
    

    Expression

    Yeast

    Mutation

    F138I, T78H

    Promoter

    PMA

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET32a-hAREG

    Plasmid

    #199229

    Purpose

    Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

    Depositor

    Nilson Zanchin

    Article

    Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

    Insert

    Amphiregulin preproprotein (AREG Human)
    

    Tags

    TrxA-6xHis-S-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDEST40-2XHA-Src-KD

    Plasmid

    #140312

    Purpose

    Expresses HA-tagged human Src-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    Contains only the KD region

    Promoter

    CMV

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. P41-bio

    Plasmid

    #47739

    Purpose

    Expresses enzymatically monobiotinylated full-length P41 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised P41
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. mKate2-P2A-APEX2-2xML1N(7Q)

    Plasmid

    #67666

    Purpose

    Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    mKate2-P2A-APEX2-ML1Nmut*2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. mRuby2-Keratin-17

    Plasmid

    #55900

    Purpose

    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Keratin (KRT18 Human)
    

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available Since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

40. pAc5.1/V5-HisA-RvCAHS12-GS-AcGFP1

    Plasmid

    #192471

    Purpose

    Transient expression of RvCAHS12 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

    Insert

    CAHS12
    

    Tags

    AcGFP1

    Expression

    Insect

    Promoter

    Ac5

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge