We narrowed to 9,393 results for: Coli

1. pSB1C3-H3

   Plasmid

   #109381

   Purpose

   Arabinose inducible VioB-mCherry expression (weaker RBS). Used to test burden response in E. coli

   Depositor

   Tom Ellis

   Article

   Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

   Insert

   pARABAD-VioB-mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPBO.ACT003

   Plasmid

   #182713

   Purpose

   aTc inducible dCasRx-IF3

   Depositor

   Joseph Schoeniger

   Article

   Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

   Insert

   dCasRx
   

   Use

   CRISPR

   Tags

   3x(GGGS)-Escherichia Coli Initiation Factor 3

   Expression

   Bacterial

   Mutation

   Catalytically deactivated R295A, H300A, R849A, H8…

   Promoter

   pTet

   Available since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHL 246

   Plasmid

   #53014

   Purpose

   AmpR + fimS (off orientation)::gfp::Asp terminator + CamR + ColE1

   Depositor

   Han Lim

   Article

   Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   fimS (off orientation)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 14, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pRSF-1b-S14

   Plasmid

   #128596

   Purpose

   Expresses tagless 30S ribosomal subunit protein S14 in E.coli

   Depositor

   James Williamson

   Article

   Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

   Insert

   30S ribosomal subunit protein S14 (rpsN E.coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PET28a-cpLumiLuc-Rapamycin sensor

   Plasmid

   #186292

   Purpose

   Expression Rapamycin sensor in e coli

   Depositor

   Kirill Alexandrov

   Article

   Guo et al Nat Commun. 2022 Feb 10;13(1):789. doi:

   Insert

   cpLumiLuc-Rapamycin sensor
   

   Use

   Tags

   HisTag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAD/HisB-PhoCl

   Plasmid

   #87688

   Purpose

   Express PhoCl in E. coli

   Depositor

   Robert Campbell

   Article

   Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   PhoCl
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTH1-SpSrtA

   Plasmid

   #64987

   Purpose

   Expresses the wild type of Streptococcus pyogenes sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

   Insert

   S. pyogenes sortase A
   

   Use

   Tags

   His6 tag, Mxe GyrA intein, and chitin-binding pro…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-81

   Promoter

   T7 promoter

   Available since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPM2212

   Plasmid

   #167552

   Purpose

   Encodes rfb region of Shigella flexneri serotype 2a, which expresses Shigella O antigen in E. coli K-12

   Depositor

   Renato Morona

   Article

   Macpherson et al Mol Microbiol. 1991 Jun;5(6):1491-9. doi

   Insert

   rfb region of Shigella flexneri serotype 2a
   

   Use

   Cosmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHL 1903

   Plasmid

   #53020

   Purpose

   AmpR + fimS::gfpAAV::Asp terminator + CamR + ColE1

   Depositor

   Han Lim

   Article

   Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   AAV, Asp terminator, and fimS

   Expression

   Bacterial

   Mutation

   Promoter

   fimS

   Available since

   May 14, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pHis-zfCSF3a

    Plasmid

    #65612

    Purpose

    express soluble His-tagged zebrafish CSF3a/Gcsfa in E. coli

    Depositor

    Petr Bartunek

    Article

    Stachura et al Blood. 2011 Aug 4;118(5):1274-82. doi: 1

    Insert

    Csf3a (csf3a Zebrafish)
    

    Use

    Tags

    (His)6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pSEVA221MBPc-AID-T7RNAP

    Plasmid

    #167975

    Purpose

    Expresses fusion protein MBPc-AID-T7RNAP in E. coli

    Depositor

    Luis Ángel Fernández

    Article

    Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

    Insert

    MBPc-AID-T7RNAP
    

    Use

    Tags

    MBPc and T7RNAP

    Expression

    Bacterial

    Mutation

    Promoter

    TetR-Ptet

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-6xHis-GFP(+24)

    Plasmid

    #199163

    Purpose

    used to express a supercharged GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+24)
    

    Use

    Tags

    MGHHHHHHGGA

    Expression

    Mutation

    E16R, V21K, D29K, E42K, E44K, D86K, D112K, T115K,…

    Promoter

    T7

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-S9

    Plasmid

    #132948

    Purpose

    Expresses N-terminal 6xHis-tagged S9 ribosomal protein in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    30S ribosomal subunit protein S9 (rpsI E.coli)
    

    Use

    Tags

    N-terminal 6xHis-tag

    Expression

    Bacterial

    Mutation

    E52C

    Promoter

    T7

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBAD-TRP120

    Plasmid

    #71732

    Purpose

    inducible expression of TRP120 in E. coli

    Depositor

    Jere McBride

    Article

    Luo et al Clin Vaccine Immunol. 2009 Jul;16(7):982

    Insert

    TRP120
    

    Use

    Tags

    6xHIS and Thioredoxin

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pXD70Tet(DadR)-DadR

    Plasmid

    #191636

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR complementation

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    DadR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET47b-T7-dAsCas12f

    Plasmid

    #204641

    Purpose

    Expression of an inactive AsCas12f in E. coli

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    inactive AsCas12f
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D225A

    Promoter

    T7

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a SV40 ST full length

    Plasmid

    #132636

    Purpose

    expresses SV40 ST full length in E. coli

    Depositor

    Wenqing Xu

    Article

    Cho et al PLoS Biol. 2007 Aug;5(8):e202. doi: 10.1

    Insert

    SV40 small t antigen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJK240

    Plasmid

    #71127

    Purpose

    Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

    Depositor

    T. J. Kappock

    Article

    Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

    Insert

    citrate synthase
    

    Use

    Tags

    GyrA intein and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDB328

    Plasmid

    #113672

    Purpose

    expresses Ulp1_R4 in Escherichia coli

    Depositor

    Christopher Bahl

    Article

    Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

    Insert

    Ulp1_R4
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    contains solubility enhancing mutations

    Promoter

    T7

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRBBm69

    Plasmid

    #48116

    Purpose

    Shuttle vector E. coli/B.meg.

    Depositor

    Dieter Jahn

    Article

    Biedendieck et al J Biotechnol. 2007 Dec 1;132(4):426-30.

    Insert

    promoter sacB - sacB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. LoriT

    Plasmid

    #163691

    Purpose

    Modified L4440 containing now CamR and oriT for conjugation

    Depositor

    Baris Tursun

    Article

    Kazmierczak et al Nucleic Acids Res. 2021 Feb 26;49(4):e22

    Insert

    oriT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMZ1001

    Plasmid

    #204398

    Purpose

    A pC-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.

    Depositor

    Lauren Andrews

    Article

    Zeng et al ACS Synth Biol. 2023 Dec 11. doi: 10.102

    Inserts

    quorum sensing regulator RpaR (rpaR )
    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PftsH and Synthetic promoter PRpa0002

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. tISR

    Plasmid

    #122664

    Purpose

    Express tISR protein in E. coli

    Depositor

    Robert Cohen

    Article

    Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Insert

    tISR (OCLM Human)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 17, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pMH-macroHC

    Plasmid

    #82337

    Purpose

    Provides a C-terminal macro domain and 6xHis tag for expression in E. coli

    Depositor

    Michael Hothorn

    Article

    Wild et al Protein Sci. 2016 Oct 24. doi: 10.1002/p

    Insert

    SPX domain of the Vacuolar Transporter Chaperone 4 (VTC4 Budding Yeast)
    

    Use

    Tags

    6xHis non-cleavable and macro domain of human his…

    Expression

    Bacterial

    Mutation

    Truncated protein, amino acids 1-178

    Promoter

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pUC19-P70a-Top-T~P70a-Bottom-T

    Plasmid

    #87314

    Purpose

    Expresses the modular Split-Broccoli system (Top + Bottom) under the control of E. coli sigma 70 promoters.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli system
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P70a

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRL2960

    Plasmid

    #70694

    Purpose

    pDU1-E. coli shuttle vector (Km-r) with extended polylinker

    Depositor

    Peter Wolk

    Article

    Wolk et al Arch Microbiol. 2007 Dec;188(6):551-63.

    Type

    Empty backbone

    Use

    Bacterial shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMH-HT_CtCHAD

    Plasmid

    #127785

    Purpose

    Expresses CtCHAD in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    CtCHAD (CT0884 Chlorobium tepidum)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Residues 208-522 (CHAD domain)

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSpyTag-GlpG

    Plasmid

    #79054

    Purpose

    Expresses SpyTagged GlpG protein

    Depositor

    James Bowie

    Article

    Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi

    Insert

    SpyTag-GlpG (glpG )
    

    Use

    Tags

    6xHis and SpyTag

    Expression

    Bacterial

    Mutation

    GlpG protein employed here corresponds to residue…

    Promoter

    Trc

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pQE30-AtAEP2

    Plasmid

    #159529

    Purpose

    Expresses Arabidopsis thaliana asparaginyl endopeptidase 2 in E. coli

    Depositor

    Joshua Mylne

    Article

    Bernath-Levin et al Chem Biol. 2015 May 21;22(5):571-82. doi

    Insert

    Arabidopsis thaliana asparaginyl endopeptidase 2
    

    Use

    Tags

    Six-Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pQE30-CeAEP1

    Plasmid

    #159528

    Purpose

    Expresses jack bean (Canavalia ensiformis) asparaginyl endopeptidase 1 in E. coli

    Depositor

    Joshua Mylne

    Article

    Bernath-Levin et al Chem Biol. 2015 May 21;22(5):571-82. doi

    Insert

    Canavalia ensiformis asparaginyl endopeptidase 1
    

    Use

    Tags

    Six-Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. BPK2014-AmCas12b

    Plasmid

    #121951

    Purpose

    E. coli expression, protein purification

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AmCas12b
    

    Use

    Tags

    10xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMGU

    Plasmid

    #139933

    Purpose

    M. magneticum/E. coli shuttle vector (pMGT and pUC)

    Depositor

    Christopher Voigt

    Article

    Furubayashi et al Adv. Funct. Mater. 2021, 31, 2004813

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23101

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRL1124

    Plasmid

    #70690

    Purpose

    provides avaiM, eco47iiM, and ecot22iM that encode specific methylases to enable transfer of plasmids from E. coli to Anabaena sp. strain PCC 7120 and other strains. lacks a mobilization function.

    Depositor

    Peter Wolk

    Article

    Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

    Inserts

    avaiM
    eco47iiM
    ecot22iM
    

    Use

    Bacterial shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMH-HT_SsCHAD

    Plasmid

    #127783

    Purpose

    Expresses SsCHAD in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    SsCHAD (SULM_RS11045 Sulfolobus solfataricus)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET28a-human Beta-catenin R1C (138–781)

    Plasmid

    #132608

    Purpose

    expresses human beta-catenin R1C (138–781) in E. coli

    Depositor

    Wenqing Xu

    Article

    Xing et al Structure. 2008 Mar;16(3):478-87. doi: 1

    Insert

    Beta-catenin R1C (138–781)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET28a-HsSENP7

    Plasmid

    #71470

    Purpose

    Encodes human full length SENP7. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 7 (SENP7 Human)
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Gene optimised for E. coli expression (Genscript)

    Promoter

    T7

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

37. pET_His6-MBP-TEV-Cas2

    Plasmid

    #186275

    Purpose

    Expresses Thiomicrospira Cas2 in E. coli

    Depositor

    Jennifer Doudna

    Article

    Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

    Insert

    Thiomicropira Cas2
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMSCV-IRES-GFP-3xFLAG-MyD88-GyrB

    Plasmid

    #131350

    Purpose

    Expresses MyD88 with an N-termial 3xFLAG tag and a C-terminal GyrB domain from the E. coli DNA Gyrase B

    Depositor

    Jonathan Kagan

    Article

    Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

    Insert

    MyD88-GyrB (Myd88 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET21b - S. thermophilus DltB

    Plasmid

    #132607

    Purpose

    expresses S. thermophilus DltB in E. coli

    Depositor

    Wenqing Xu

    Article

    Ma et al Nature. 2018 Oct;562(7726):286-290. doi:

    Insert

    Dltb
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pQE30-HaAEP1

    Plasmid

    #159530

    Purpose

    Expresses sunflower (Helianthus annuus) asparaginyl endopeptidase 1 in E. coli

    Depositor

    Joshua Mylne

    Article

    Bernath-Levin et al Chem Biol. 2015 May 21;22(5):571-82. doi

    Insert

    Helianthus annuus asparaginyl endopeptidase 1 (LOC110922574 Synthetic)
    

    Use

    Tags

    Six-Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge