We narrowed to 10,975 results for: NSI;

1. pVL90 - VND7

   Plasmid

   #116035

   Purpose

   entry vector for GreenGate cloning method, CDS

   Depositor

   Thomas Greb

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   VND7 (VND7 Mustard Weed)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. BRCA1-(1234-1443)

   Plasmid

   #35078

   Depositor

   Cheryl Arrowsmith

   Article

   Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

   Insert

   BRCA1 (BRCA1 Human)
   

   Use

   Tags

   His

   Expression

   Mutation

   Contains aa 1234-1443

   Promoter

   T7

   Available since

   April 4, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pLentiCRISPR sgFTCD_1

   Plasmid

   #102312

   Purpose

   genetic depletion of FTCD

   Depositor

   David Sabatini

   Article

   Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   sgFTCD_1 (FTCD Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLentiCRISPR sgFTCD_2

   Plasmid

   #102313

   Purpose

   genetic depletion of FTCD

   Depositor

   David Sabatini

   Article

   Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   sgFTCD_2 (FTCD Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pOPTXGARE

   Plasmid

   #68542

   Purpose

   cyclic nucleotide reporter for bacteria or plant cells

   Depositor

   Helen Irving

   Article

   Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

   Insert

   OPTX promoter with GARE (AT1G33440 Mustard Weed)
   

   Use

   Tags

   luciferase

   Expression

   Bacterial and Plant

   Mutation

   5x GARE inserted 190 bp 5' pf ATG (GARE = ta…

   Promoter

   OPTX promoter with GARE

   Available since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. M26I DJ-1 TEV site pET15b

   Plasmid

   #60685

   Purpose

   Recombinant protein expression of Park7

   Depositor

   Mark Wilson

   Article

   Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

   Insert

   M26I DJ-1 (PARK7 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M26I and TEV site was inserted (see comments belo…

   Promoter

   T7

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pRKVSV Rac T17N

   Plasmid

   #50537

   Depositor

   Kenneth Yamada

   Article

   Koivisto et al Exp Cell Res. 2004 Feb 1;293(1):68-80.

   Insert

   small GTPase, mutated (RAC1 Human)
   

   Use

   Tags

   2X VSV

   Expression

   Mammalian

   Mutation

   T17N (dominant negative)

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pEM-Cas9noSsrA

   Plasmid

   #102285

   Purpose

   Modified from pCas9-CR4 (Addgene: 62655) to remove the ssrA tag from Cas9.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   removed ssrA tag

   Promoter

   pTet

   Available since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVRa37_2513

   Plasmid

   #49696

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   ECF37_2513
   

   Use

   Synthetic Biology

   Tags

   His6-PreScission

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pT7-lacO

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCELF PAI-RBP v3

    Plasmid

    #45507

    Depositor

    Thomas Gelehrter

    Article

    Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

    Insert

    PAI-RBP v3 (SERBP1 Human, Rat)
    

    Use

    Tags

    GST tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pCELF PAI-RBP v1

    Plasmid

    #45506

    Depositor

    Thomas Gelehrter

    Article

    Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

    Insert

    PAI-RBP v1 (SERBP1 Human, Rat)
    

    Use

    Tags

    GST tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pCELF PAI-RBP v2

    Plasmid

    #45505

    Depositor

    Thomas Gelehrter

    Article

    Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

    Insert

    PAI-RBP v2 (SERBP1 Human, Rat)
    

    Use

    Tags

    GST tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2013

    Availability

    Academic Institutions and Nonprofits only

13. M26L DJ-1 TEV site pET15b

    Plasmid

    #60686

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    M26L DJ-1 (PARK7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M26L and TEV site was inserted (see comments belo…

    Promoter

    T7

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pBABEpuro-ARAF_S214F

    Plasmid

    #51078

    Purpose

    mammalian expression of ARAF S214F

    Depositor

    Matthew Meyerson

    Article

    Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

    Insert

    ARAF (ARAF Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    S214F

    Promoter

    Available since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Tip alpha K102A/K104A (21-192)

    Plasmid

    #23159

    Depositor

    SeWon Suh

    Article

    Jang et al J Mol Biol. 2009 Sep 11. 392(1):191-7.

    Insert

    TNF-α-inducing protein
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    changed lysine 102 and lysine 104 to alanine, and…

    Promoter

    Available since

    April 16, 2010

    Availability

    Academic Institutions and Nonprofits only

16. BRCA1-(1057-1234)

    Plasmid

    #35076

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Mutation

    Contains aa 1057-1234

    Promoter

    T7

    Available since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-Rog

    Plasmid

    #24429

    Depositor

    Bruce Conklin

    Article

    Scearce-Levie et al BMC Biol. 2005 . 3():3.

    Insert

    RASSL (OPRK1 Human)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    mutations in extracellular loops 2 (see comments …

    Promoter

    Available since

    June 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Immunostealth

    Plasmid

    #239446

    Purpose

    Expresses murine NIS (mNIS) in mammalian cells for non-immunogenic in vivo imaging using SPECT or PET

    Depositor

    Scott Lyons

    Article

    Merrill et al Cell Stress. 2023 Aug 14;7(8):59-68. doi

    Insert

    sodium iodide symporter (Slc5a5 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRCA905 - KLF4 Puro-T2A-BFP (pRCA847 backbone)

    Plasmid

    #238185

    Purpose

    Lentiviral vector for overexpression KLF4 ORF from a EF1a promoter

    Depositor

    Thomas Norman

    Article

    Southard et al bioRxiv [Preprint]. 2024 Aug 3:2024.07.3

    Insert

    KLF4 (KLF4 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRCA879 - KLF5 isoform 1 Puro-T2A-GFP (pRCA845 backbone)

    Plasmid

    #238181

    Purpose

    Lentiviral vector for overexpression KLF5 ORF from a EF1a promoter

    Depositor

    Thomas Norman

    Article

    Southard et al bioRxiv [Preprint]. 2024 Aug 3:2024.07.3

    Insert

    KLF5 (KLF5 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRCA903 - KLF5 isoform 1 Puro-T2A-BFP (pRCA845 backbone)

    Plasmid

    #238184

    Purpose

    Lentiviral vector for overexpression KLF5 ORF from a EF1a promoter

    Depositor

    Thomas Norman

    Article

    Southard et al bioRxiv [Preprint]. 2024 Aug 3:2024.07.3

    Insert

    KLF5 (KLF5 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRCA376 - pBA904 Puro-T2A-GFP NTC guide (pRCA360 backbone)

    Plasmid

    #238167

    Purpose

    Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

    Depositor

    Thomas Norman

    Article

    Southard et al bioRxiv [Preprint]. 2024 Aug 3:2024.07.3

    Insert

    Non-targeting sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRCA881 - KLF4 Puro-T2A-GFP (pRCA847 backbone)

    Plasmid

    #238182

    Purpose

    Lentiviral vector for overexpression KLF4 ORF from a EF1a promoter

    Depositor

    Thomas Norman

    Article

    Southard et al bioRxiv [Preprint]. 2024 Aug 3:2024.07.3

    Insert

    KLF4 (KLF4 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p300 KAT LIC 1M

    Plasmid

    #233587

    Purpose

    Bacterial expression of p300 KAT enzyme

    Depositor

    Scott Showalter

    Article

    Dewing et al Proteins. 2025 Jun 1. doi: 10.1002/prot.

    Insert

    p300 KAT (EP300 Human)
    

    Use

    Tags

    6xHis MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pICH47822_Myc-Sr62TK

    Plasmid

    #233536

    Purpose

    Expresses Myc-Sr62TK in planta

    Depositor

    Brande Wulff

    Article

    Chen et al bioRxiv 2024.08.30.610287

    Insert

    Sr62TK
    

    Use

    Tags

    Myc tag

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p3xFLAG-CMV-14-GINIP-Nluc W139A

    Plasmid

    #223557

    Purpose

    Plasmid expressing GINIP-Nluc W139A in mammalian cells

    Depositor

    Mikel Garcia-Marcos

    Article

    Luebbers et al Sci Signal. 2025 Mar 25;18(879):eadp6457

    Insert

    human GINIP W139A - linker(GGGS) - Nluc
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    W139A mutation in GINIP sequence

    Promoter

    Available since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pECNUS4_MP

    Plasmid

    #228358

    Purpose

    ABE8e-SpCas9-NG Multiplex Acceptor clone for insertion of up to six gRNA cassettes via Golden Gate with BpiI and T4 ligase, blue-white screening

    Depositor

    Eunyoung Chae

    Article

    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    RPS5A

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTol1 - 14xUAS:CaSR-EGFP

    Plasmid

    #191502

    Purpose

    A UAS construct used to create transgenic fish that express CaSR-EGFP when crossed to a fish carrying a Gal4.

    Depositor

    Michael Granato

    Article

    Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

    Insert

    CaSR-EGFP-SV40
    

    Use

    Creation of transgenic fish using tol1 transgenes…

    Tags

    Expression

    Mutation

    Promoter

    14X UAS

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMRX-IP-hWIPI3-3×FLAG

    Plasmid

    #215511

    Purpose

    Expresses FLAG tagged human WIPI3.

    Depositor

    Noboru Mizushima

    Article

    Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

    Insert

    WD-repeat protein interacting with phosphoinositides 3 (WDR45B Human)
    

    Use

    Retroviral

    Tags

    3×FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-CAG-UnaG-P2A-FusionRed

    Plasmid

    #191109

    Purpose

    Mammalian cell line expression plasmids for production of UnaG and FusionRed proteins

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    UnaG
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    chicken β-actin promoter

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHR-SNAP-CD86iso2

    Plasmid

    #223574

    Purpose

    Lentiviral expression of human CD86 isoform 2 with SNAP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

    Insert

    CD86 (CD86 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    SNAP

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRSF_ApyADO

    Plasmid

    #226248

    Purpose

    Production of ApyA peptide modified by the B12-rSAM enzyme ApyD and the cytochrome P450 enzyme ApyO

    Depositor

    Wilfred van der Donk

    Article

    Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

    Inserts

    His6_ApyA
    ApyD
    ApyO
    

    Use

    Tags

    Hexa-Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRSF_ApyAO

    Plasmid

    #226242

    Purpose

    Production of ApyA peptide modified by the cytochrome P450 enzyme ApyO

    Depositor

    Wilfred van der Donk

    Article

    Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

    Inserts

    His6_ApyA
    ApyO
    

    Use

    Tags

    Hexa-Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRSF_ApyAD

    Plasmid

    #226241

    Purpose

    Production of ApyA peptide modified by the B12-rSAM enzyme ApyD

    Depositor

    Wilfred van der Donk

    Article

    Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

    Inserts

    His6_ApyA
    ApyD
    

    Use

    Tags

    Hexa-Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pJB50

    Plasmid

    #226311

    Purpose

    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    CsoS2 with V(T/S)G to AAA CsoS2 MR1-7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…

    Promoter

    pTRC

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJB55

    Plasmid

    #226310

    Purpose

    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    CsoS2 with V(T/S)G to AAA CsoS2 MR1-5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…

    Promoter

    pTRC

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTE5419_p3WJdB-glcO36

    Plasmid

    #221193

    Purpose

    GlcR module template with glcO36 operator: T7 promoDNA template of GlcR sensor with glcO36 operator: T7 promoter-glcO36-3WJdB reporter-T7 terminator linear DNA tter-glcO36-3WJdB reporter-T7 terminator

    Depositor

    Tobias Erb

    Article

    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

    Insert

    T7 promoter-glcO36
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter with downstream glcO36 operator

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGEX-PML B-box1 WT

    Plasmid

    #185632

    Purpose

    expression of GST-tagged wild-type PML B-box1 domain in bacterial cells

    Depositor

    Irina Bezsonova

    Article

    Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

    Insert

    promyelocytic leukemia protein (PML Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGEX-PML B-box1 C4C4

    Plasmid

    #185640

    Purpose

    expression of GST-tagged mutant PML B-box1 H155C/H161C domain in bacterial cells

    Depositor

    Irina Bezsonova

    Article

    Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

    Insert

    promyelocytic leukemia protein (PML Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    H155C H161C

    Promoter

    Available since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGEX-PML B-box1 W157E

    Plasmid

    #185641

    Purpose

    expression of GST-tagged mutant PML B-box1 W157E domain in bacterial cells

    Depositor

    Irina Bezsonova

    Article

    Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

    Insert

    promyelocytic leukemia protein (PML Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    W157E

    Promoter

    Available since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge