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Plasmid#195346PurposeControl plasmid for the MscL tension biosensorDepositorInsertMscL(G22S)-c-term-cpGFP-ERexp
UseLentiviralTagsExpressionMammalianMutationPromoterCMVd2Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBFv-UAS3
Plasmid#138399Purpose10xUAS expression vector with a vermilion marker and a phiC31 attB sequenceDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterUASAvailable sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ecoli cpGFP
Plasmid#215713PurposeExpresses cpGFP in EcoliDepositorInsertcpGFP
UseTags6xHisExpressionBacterialMutationcircularly permuted GFPPromoterT7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cherry-ICP0-C3
Plasmid#134560PurposeExpresses Cherry-ICP0 fusion proteinDepositorInsertICP0
UseTagsCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GFP(S65T)-TRP1
Plasmid#41597DepositorInsertTRP1 (TRP1 Budding Yeast)
UseYeast genomic targetingTagsGFP (S65T) and yeast ADH1 terminatorExpressionMutationPromoterAvailable sinceJan. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-13Myc-TRP1
Plasmid#41601DepositorInsertTRP1 (TRP1 Budding Yeast)
UseYeast genomic targetingTags13Myc and yeast ADH1 terminatorExpressionMutationPromoterAvailable sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFPN3-Sstr3
Plasmid#35623DepositorInsertSstr3 (Sstr3 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-bActinCDS-bActinUTR-MS2V5
Plasmid#102718PurposeHalo-Actin reporter for detection of both mRNA and proteinDepositorInsertHaloTag-bActinCDS-bActin3'UTR-MS2V5
UseLentiviralTagsHaloTag and MS2V5ExpressionMutationPromoterUbCAvailable sinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-CLIPtag
Plasmid#179787PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertCLIP
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mhYFP
Plasmid#186533PurposeMonomeric hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cellsDepositorInsertMonomeric hyperfolder YFP
UseTagsExpressionMammalianMutationhfYFP-S147P/L195M/V206KPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDL278_P23-DsRed-Express2
Plasmid#121505PurposeE. coli-streptococcal shuttle vector that expresses rfpDepositorInsertDsRed-Express2
UseE. coli streptococcal shuttle vectorTagsExpressionMutationPromoterP23Available sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
2xMARS
Plasmid#205233PurposeExpresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-iin mammalian cellsDepositorInserttwo repeats of PLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)
UseTagsNuclear Export Sequence and mScarlet-iExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMito.iGABASnFR
Plasmid#112175PurposeGABA SensorDepositorInsertiGABASnFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lv EF1a MS2-Fkbp2x 2A Hygro
Plasmid#102807PurposeLentiviral plasmid from FIRE-Cas9 system to express MS2-Fkbp2x fusion proteinDepositorInsertMS2-Fkbp2x
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLN-AIDA-Tyr1
Plasmid#192831PurposeIPTG inducible AIDA-Tyrosinase expressionDepositorInsertAIDA-Tyr1
UseTagsExpressionBacterialMutationPromoterpL-lacAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pM-mTagBFP2
Plasmid#228615PurposeMammalian expression of mTagBFP2 and extra insert, for MuSIC barcode constructionDepositorInsertmTagBFP2
UseTagsExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-mNeon-TurboID
Plasmid#186610PurposeThird generation lentiviral vector expressing mNeonGreen fused to V5-TurboIDDepositorInsertmNeon-V5-TurboID
UseLentiviralTagsmNeonGreenExpressionMammalianMutationFor reference see Branon et al., 2018PromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCin4-3xFLAG-MKL1
Plasmid#19838DepositorInsertMKL1 (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCAMBgfp
Plasmid#206130PurposeThis binary vector has been generated for introduction of SGFP in filamentous fungi using ATMT. Contains the hygromycin resistance gene and the SGFP gene.DepositorInserthygromycin resistance gene and the Promoter of ToxA+SGFP
UseBinary vector for expression of sgfp in filamento…TagsExpressionMutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3E-APEX2-P2A-EGFP
Plasmid#108896PurposeMultisite gateway vector for 3' tagging with APEX2 (includes cytoplasmic GFP reporter)DepositorInsertAPEX2-P2A-EGFP
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT03
Plasmid#223375PurposeT-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.DepositorInsert2x35s-SpCas9-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT05
Plasmid#223377PurposeT-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.DepositorInsertZmUbi-SpCas9-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pR-CyOFP1
Plasmid#228604PurposeMammalian expression of CyOFP1, for MuSIC barcode constructionDepositorInsertCyOFP1
UseTagsExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Bxb1_tdTomato
Plasmid#198041PurposeBxb1-specific donor plasmid for integrating a transcription unit for constitutive expression of tdTomato reporter.DepositorInserttdTomato
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ki67-mNeonGreen-IRESpuro2
Plasmid#183737PurposeExpresses Ki67-mNeonGreenDepositorInsertMKI67 (MKI67 Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBad-CA
Plasmid#105665Purposeexpresses thermostable variant of human carbonic anhydrase II with C-terminal 6x his tagDepositorArticleInserthuman carbonic anhydrase II thermostable variant (CA2 Human)
UseTags6x his tagExpressionBacterialMutationPromoterpBad/arabinoseAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKISox1-P2A-eGFP-Hygro
Plasmid#115685PurposeKnock-in of P2A-eGFP at the mouse Sox1 geneDepositorInsertP2A-eGFP-loxP-PGK-Hygro-loxP
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
TRPML3-YFP
Plasmid#18835DepositorInsertTRPML3 (Mcoln3 Mouse)
UseTagsYFPExpressionMammalianMutationQ165R, F210YPromoterAvailable sinceJuly 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
4xNLS-pMJ915v2
Plasmid#88917PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmtb-t7-alpha-bungarotoxin
Plasmid#69542PurposeFor in vitro transcription of alpha-bungarotoxin mRNA that can be injected into embryos.DepositorInsertalpha-bungarotoxin
UseTol2, beta-actin promoter, sp6,t7 for zebrafishTagsExpressionMutationPromoterbeta-actin, sp6, t7Available sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato
Plasmid#60658PurposeFlp-dependent expression of mNaChBac-T2A-tdTomatoDepositorHas ServiceAAV8InsertFLEX-mNaChBac-T2A-tdTomato
UseAAVTagsExpressionMutationPromoterEF1αAvailable sinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-cOVA
Plasmid#25097DepositorInsertcytoplasmic ovalbumin
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
SuperNova-N1
Plasmid#131853PurposeExpresses SuperNova with C-terminal Myc-tag and N-terminal multiple cloning siteDepositorInsertSuperNova
UseTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRE-ChrimsonR-mCherry
Plasmid#92207PurposeReporter constructDepositorInsertChimsonR-mCherry
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceJune 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAGM22082_sfGFP1-10
Plasmid#153515PurposeE.coli expression plasmid for the production of GFP1-10DepositorInsertsfGFP1-10
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCN-EF2132tet
Plasmid#107191PurposeExpresses EF2132 recombinase for performing recombineering in S. aureusDepositorInsertsEF2132
tetR
UseTagsExpressionBacterialMutationPromoterp23Available sinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBR-Lent-STAT5A-CA
Plasmid#170662PurposeLentiviral expression of constitutively active STAT5A.DepositorInsertSTAT5A (STAT5A Human)
UseLentiviralTagsExpressionMutationH298R and S710FPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
KBB460
Plasmid#185094PurposeNMA111-GFP wild type under endogenous promoterDepositorInsertNMA111
UseTagsGFPExpressionYeastMutationNMA111-GFPPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
GfaABC1D-cyto-iATPSnFR1.0
Plasmid#102553Purposeexpresses cytoplasmic ATP sensor in astrocytesDepositorInsertiATPSnFR1.0
UseAAVTagsExpressionMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL3-pegRNA-attL2
Plasmid#213052PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL3 and attL2DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only