We narrowed to 88,170 results for: Mal

1. pcDNA3.1-dCas9-CibN

   Plasmid

   #60552

   Purpose

   Expresses dCas9-CibN in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   dCas9-CibN
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   inactivating Cas9 mutations D10A and H840A

   Promoter

   CMV

   Available Since

   Jan. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSicoR-ADAM29i1

   Plasmid

   #59606

   Purpose

   Expression of shRNA against human ADAM29

   Depositor

   Miguel Ramalho-Santos

   Article

   Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

   Insert

   ADAM29 (ADAM29 Human)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pTS99 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-3C-MCS

   Plasmid

   #199352

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-3C-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TagBFP-P2A-ALFA-3C

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTS111 pHAGE2 CMVtetO2 MCS-3C-22xGS-Alfa-P2A-TagBFP

   Plasmid

   #199364

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-ALFA-P2A-BFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-ALFA-P2A-TagBFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LbCas12a-DR-cr in PBCSK-

   Plasmid

   #126641

   Purpose

   Cloning vector LbCas12a DR-crRNA for expression in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   LbCas12a Full Length Direct Repeat crRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 Promoter

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. All-in-one split-ADAR2

   Plasmid

   #170136

   Purpose

   AAV vector carrying ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCS2-mCherry_SNAP_SNAPV_PACT_Dead

   Plasmid

   #173073

   Purpose

   Dead SNAP tag at the centrosome

   Depositor

   Heidi Hehnly

   Article

   Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 1

   Insert

   mCherry-SNAP-SNAP-Varied-PACT
   

   Use

   Tags

   mCherry-SNAP-SNAP-Varied

   Expression

   Mammalian

   Mutation

   AA144 in both SNAP tags changed from a cysteine t…

   Promoter

   CMV IE94

   Available Since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-HCMVgH (Merlin)

   Plasmid

   #136448

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein H (Merlin strain), full-length

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   glycoprotein H (UL75 Human betaherpesvirus 5)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJZC42

   Plasmid

   #66564

   Purpose

   sgRNA + 1XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Inserts

   sgRNA + 1XPP7
   PCP-VP64 IRES mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GluR2_adRNA(1,20,6)-ADAR2

    Plasmid

    #124620

    Purpose

    Expresses adRNA (1,20,6) targeting the RAB7A transcript along with the ADAR2

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

    Insert

    ADAR2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-SMVP-Cas9N-U6-gRNA TdL

    Plasmid

    #80938

    Purpose

    Expresses Cas9N in mammalian cells; expresses gRNA TdL for Ai9 cleavage.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. dCas9-NLS-gs10-cGR2176

    Plasmid

    #188258

    Purpose

    Plasmid ensures constitutive expression of the C-terminal fragment of split GR2 (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

    Depositor

    Roman Jerala

    Article

    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

    Insert

    cGR2176
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FUp95GW (B7)

    Plasmid

    #74016

    Purpose

    lentiviral expression of shRNA resistant Psd95-EGFP fusion

    Depositor

    Oliver Schluter, Robert Malenka, Weifeng Xu

    Article

    Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

    Insert

    Psd95 (DLG4 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDNAIIIB rhoA WT

    Plasmid

    #61631

    Purpose

    mammalian expression of rhoA

    Depositor

    JS Gutkind

    Article

    Coso et al Cell. 1995 Jun 30;81(7):1137-46.

    Insert

    rhoA (RHOA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

15. GWF180

    Plasmid

    #133607

    Purpose

    CAG-DNCR2-VPR-IRES-Puro-WPRE-SV40PA-PGK-NS3aH1-tagBFP-SpdCas9

    Depositor

    Dustin Maly

    Article

    Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

    Insert

    DNCR2-VPR, NS3aH1-tagBFP-SpdCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDNA3-Flag-hDcp2

    Plasmid

    #72213

    Purpose

    mammalian expression of Flag-tagged human Dcp2

    Depositor

    Megerditch Kiledjian

    Article

    Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

    Insert

    Dcp2 (DCP2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Q170R (please see depositor's comment below)

    Promoter

    CMV

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. OVA-miSFIT-8-G

    Plasmid

    #124675

    Purpose

    Tuning expression of cytosolic Ovalbumin coupled to EGFP

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    Cyto-Ovalbumin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Truncated OVA for cytoplasmic expression

    Promoter

    minimal CMV

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFP-Myo 1e

    Plasmid

    #134905

    Purpose

    Expression of rat Myo 1e (Myr 3) in mammalian cells.

    Depositor

    Martin Bähler

    Article

    Stoffler et al J Cell Biol. 1995 May;129(3):819-30. doi

    Insert

    Myo 1e (rat) with 3' UTR (Myo1e Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pQsupR-Mi2b

    Plasmid

    #15379

    Depositor

    Stephen Smale

    Article

    Ramirez-Carrozzi et al Genes Dev. 2006 Feb 1. 20(3):282-96.

    Insert

    short hairpin RNA against mouse Mi-2 beta (Chd4 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 18, 2008

    Availability

    Academic Institutions and Nonprofits only

20. BoxB-MS2 adRNA (RAB7A-ACG site)

    Plasmid

    #170155

    Purpose

    AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript for C-U editing

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    BoxB-RAB7A(ACG)-MS2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available Since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge