We narrowed to 7,519 results for: Lif

1. pPSup_35Senh(fwd)_35Spr+synJ

   Plasmid

   #149422

   Purpose

   Plasmid for STARR-seq in tobacco leaves. The GFP reporter gene is under the control of a 35S minimal promoter and an upstream 35S core enhancer.

   Depositor

   Stan Fields

   Article

   Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

   Insert

   35S core enhancer
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2-mCherry-Rab11a (S25N)

   Plasmid

   #184036

   Purpose

   Expresses dominate negative mCherry Rab11a in mammalian cells and zebrafish embryos

   Depositor

   Heidi Hehnly

   Article

   Krishnan et al Life Sci Alliance. 2022 Mar 18;5(7). pii

   Insert

   Rab11
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Serine 25 changed to Asparagine

   Promoter

   CMV IE94

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFR-mScarletI-antiFRET-P2A-G-Ca-FLITS

   Plasmid

   #191457

   Purpose

   Compare the intensity of the genetically encoded calcium sensor (GECI) G-Ca-FLITS to mScarlet-I fluorescent protein

   Depositor

   Dorus Gadella

   Article

   van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

   Insert

   G-Ca-FLITS
   

   Use

   Tags

   His-mScarletI-antiFRETlinker-P2A

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-3xflagGREB1a

   Plasmid

   #112221

   Purpose

   mammalian expression of flag tagged GREB1a

   Depositor

   Craig Burd

   Article

   Haines et al Endocr Relat Cancer. 2018 Jul;25(7):735-

   Insert

   GREB1a (GREB1 Human)
   

   Use

   Tags

   3x flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. STOP-eGFP-ROSA26TV

   Plasmid

   #11739

   Depositor

   Klaus Rajewsky

   Article

   Sasaki et al Immunity. 2006 Jun . 24(6):729-39.

   Insert

   STOP-eGFP-ROSA26TV
   

   Use

   Cre/Lox; /stop

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZS*11_4enh

   Plasmid

   #149423

   Purpose

   Plasmid harboring four plant enhancers (35S, AB80, Cab-1, and rbcS-E9).

   Depositor

   Stan Fields

   Article

   Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

   Inserts

   rbcS-E9 enhancer
   35S core enhancer
   Cab-1 enhancer
   AB80 enhancer
   

   Use

   Tags

   T120>A

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKD184

   Plasmid

   #90953

   Purpose

   Expresses ThsR under the PltetO-1 promoter, sfGFP under PphsA, and mCherry constitutively

   Depositor

   Jeffrey Tabor

   Article

   Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

   Inserts

   Thiosulfate response regulator
   Superfolder GFP
   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Bba_J23114, PltetO-1, and PphsA

   Available since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. SDHC-pHLuorin-sems

   Plasmid

   #107767

   Purpose

   labeling of mitochondrial succinate dehydrogenase subunit C, pH measurements

   Depositor

   Karin Busch

   Article

   Rieger et al Sci Rep. 2017 Apr 6;7:46055. doi: 10.103

   Insert

   Subunit C of SDH fused to pHluorin (SDHC Human)
   

   Use

   Tags

   pHluorin

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. NET29A.Hs pEGFP-N2 (1181)

   Plasmid

   #62040

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

   Insert

   NET29A (TMEM120A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Splice variant

   Promoter

   CMV

   Available since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBITE-EGFRvIII-F271-OKT3

    Plasmid

    #190682

    Purpose

    Encodes an EGFRvIII-CD3 BITE protein (F271 clone)

    Depositor

    Scott McComb

    Article

    Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

    Insert

    Anti-EGFRvIII-scFv-F271-modularized-OKT3-HisTag
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXSE901BG

    Plasmid

    #91714

    Purpose

    CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta and glyphosate-resistance

    Depositor

    Qi-Jun Chen

    Article

    Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. p403-BrdU-Inc

    Plasmid

    #71789

    Purpose

    BrdU-incorporating plasmid with selectable marker HIS3

    Depositor

    Oscar Aparicio

    Article

    Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

    Inserts

    Thymidine Kinase
    ENT1 (SLC29A1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 and GPD

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA5/FRT/TO_CalR-HaloTag7-P30-SNAP-tag-KDEL

    Plasmid

    #182012

    Purpose

    CMV overexpression of HaloTag7 and SNAP-tag fusion in the endoplasmic reticulum of mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    CalR-HaloTag7-P30-SNAP-tag-KDEL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti6.3_CRKL-WT-V5

    Plasmid

    #196018

    Purpose

    Expresses CRKL WT, V5 tagged

    Depositor

    William Hahn

    Article

    Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

    Insert

    CRKL
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWPI-E2F3

    Plasmid

    #114298

    Purpose

    Constitutive expression of the E2F3 protein

    Depositor

    Patrick Salmon

    Article

    Rady et al Cell Cycle. 2013 Aug 15;12(16):2691-702.

    Insert

    E2F3 (E2F3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. p404-BrdU-Inc

    Plasmid

    #71790

    Purpose

    BrdU-incorporating plasmid with selectable marker TRP1

    Depositor

    Oscar Aparicio

    Article

    Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

    Inserts

    Thymidine Kinase
    ENT1 (SLC29A1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 and GPD

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLAC

    Plasmid

    #45072

    Purpose

    lacritin-intein fusion plasmid coding for intact mature lacritin without signal peptide

    Depositor

    Gordon Laurie

    Article

    Wang et al J Cell Biol. 2006 Aug 28;174(5):689-700.

    Insert

    Lacritin (LACRT Human)
    

    Use

    Protein expression vector

    Tags

    Intein

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV-Hygromycin-TTF-1

    Plasmid

    #119173

    Purpose

    Retroviral mammalian expression vector containing wt-human TTF-1 cDNA

    Depositor

    David Mu

    Article

    Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Retroviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBSc + MShal

    Plasmid

    #16166

    Depositor

    Larry Salkoff

    Article

    Pak et al Proc Natl Acad Sci U S A. 1991 May 15. 8

    Insert

    Mus musculus potassium voltage-gated channel (Kcnd1 Mouse)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pCMV-3XFLAG-renilla-luciferase-beta-globin-(39PTC)

    Plasmid

    #184394

    Purpose

    Transient transfection plasmid expressing Renilla-luciferase-beta-globin fusion protein with PTC in beta-globin

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    Renilla luciferase
    

    Use

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pT2Aneo-ERT2CreERT2

    Plasmid

    #173850

    Purpose

    Encoding Cre recombinase (activated in response to tamoxifen)

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    ERT2CreERT2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDuRCC-N

    Plasmid

    #81194

    Purpose

    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please view depositor comments below and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLenti6.3_CRKL-Y207F-V5

    Plasmid

    #196016

    Purpose

    Expresses CRKL Y207F, V5 tagged

    Depositor

    William Hahn

    Article

    Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

    Insert

    CRKL
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pPBpuro-ErbB4

    Plasmid

    #197363

    Purpose

    Encoding human ErbB4.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    ERBB4 (ERBB4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDUP1

    Plasmid

    #223516

    Purpose

    inactive cogfp under control of Ptet, cogfp under control of Ptac

    Depositor

    Yolanda Schaerli

    Article

    Ljiljana et al eLife13:RP97216

    Inserts

    CoGFP
    CoGFP inactivated
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    anti-dimerization mutations S129G, 22 C154S, D156…

    Promoter

    Ptac, Ptac and Ptet, and Ptet

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pENTR1A hu_ncOGT WT

    Plasmid

    #154281

    Purpose

    Entry vector encoding non-tagged O-GlcNAc Transferase

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway entry vector

    Tags

    2x FLAG and mKate2

    Expression

    Mutation

    WT OGT

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pXWJ109As(RS-te-AAV)

    Plasmid

    #123156

    Purpose

    For TEV-based leakiness regulation. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109, ParsR, PhrpLE

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLV-EF1a-DHB-mTurq2

    Plasmid

    #212667

    Purpose

    Express tagged DHB

    Depositor

    Yumi Konagaya, Tobias Meyer

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    DHB (HELB Human)
    

    Use

    Lentiviral

    Tags

    mTurq2

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pOPO717

    Plasmid

    #195302

    Purpose

    pBAD322-MycaIDCas-lacZsp5, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is lacZ spacer 5.

    Depositor

    Joseph Peters

    Article

    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

    Insert

    McCAST Cascade and lacZ spacer 5 in native array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Arabinose inducible arapBAD

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAG306-GPD-VMA1 chr I

    Plasmid

    #41896

    Purpose

    Yeast expression of VMA1.

    Depositor

    Dan Gottschling

    Article

    Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

    Insert

    VMA1 (VMA1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Contains silent mutation at 342A-G and L87S mutat…

    Promoter

    GPD

    Available since

    April 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge