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We narrowed to 19,107 results for: Tec

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Showing: 7951 - 8000 of 19107 results

  3. pCMV-eGFP-mPlum-HA

    Plasmid
    #200009
    Purpose
    Donor vector with double fluorescence system for targeted integration using CRISPR/Cas9. Expresses 5´HA--eGFP&PuroR--3´HA--mPlum. Distinguish targeted from random integration by fluorescence.
    Depositor
    Thomas Noll
    Article
    Hertel et al Front Bioeng Biotechnol. 2022 Oct 13;10:
    Insert
    eGFP, mPlum
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    200009-plasmid-map-sequence-id-398431

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  6. MSP2133

    Plasmid
    #72249
    Purpose
    Human expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    Y450A, N497A, R661A, Q695A, and Q926A
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72249-plasmid-map-sequence-id-131869

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  22. pCEP4-HA-Flag-natT1R2 FS1

    Plasmid
    #113955
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    delete N-terminal 21 residues, one base pair dele…
    Promoter
    cmv
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113955-plasmid-map-sequence-id-215169

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  26. SpCas9-NG-APOBEC-nCas9-Ung

    Plasmid
    #198539
    Purpose
    Expresses human codon-optimized SpCas9-NG-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NG-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-APOBEC-nCas9-Ung
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198539-plasmid-map-sequence-id-388890

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  31. pGM202T7

    Plasmid
    #69993
    Purpose
    E. coli/Streptomyces expression vector containing the T7-promoter
    Depositor
    Günther Muth
    Article
    Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(
    Inserts
    aphII
    tsr
    rep
    sso
    T7 promoter
    dso
    His-Orf
    Tags
    N-terminal and C-terminal His tag encoding sequen…
    Expression
    Bacterial
    Available Since
    Nov. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    69993-plasmid-map-sequence-id-129665

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  32. pLCHKO_HPRT1_exon2_1_Lb

    Plasmid
    #155051
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of HPRT1 exon 2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155051-plasmid-map-sequence-id-308437

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  37. pAAV-TRE-DIO-GCaMP6s

    Plasmid
    #183809
    Purpose
    his plasmid is for use with neuronal cell-type selective activity tagging. The GCaMP6 expression requires both neural activity and Cre recombinase.
    Depositor
    William Wisden
    Article
    Yu et al Science. 2022 Jul;377(6601):63-72. doi:
    Insert
    AAV transgene TetO promoter-DIO/flex-GCaMP6
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Expression
    Mammalian
    Available Since
    July 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    183809-plasmid-map-sequence-id-366066

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  39. MSP2135

    Plasmid
    #72248
    Purpose
    Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, and D1135E
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72248-plasmid-map-sequence-id-131868

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  41. pLCHKO_HPRT1_exon3_1_Lb

    Plasmid
    #155053
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of HPRT1 exon 3
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155053-plasmid-map-sequence-id-308427

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  47. SpCas9-NRCH-SsAPOBEC3B

    Plasmid
    #198550
    Purpose
    Expresses human codon-optimized SpCas9-NRCH-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NRCH-SsAPOBEC3B-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRCH-SsAPOBEC3B
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198550-plasmid-map-sequence-id-388894

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  49. MuHKI-pGFPN3

    Plasmid
    #21919
    Depositor
    Hossein Ardehali
    Article
    Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.
    Insert
    Mutant huamn HKI (with a mutation in the catalytic site in the C-terminal half of the enzyme) in pGFP-N3 (HK1 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This is the full length human HKI cDNA sequence w…
    Available Since
    Nov. 2, 2009
    Availability
    Academic Institutions and Nonprofits only
Showing: 7951 - 8000 of 19107 results