We narrowed to 27,277 results for: Tat

1. pLBT0110

   Plasmid

   #172807

   Purpose

   Level 1 module containing 35S-deltaPTAC5-cYFP_P3 F

   Depositor

   Benjamin Field

   Article

   Velay et al Plant Methods. 2022 May 26;18(1):69. doi

   Insert

   p35S-5UTMV-CTPSSU-deltamPTAC5-cYFP3XHA-3U+TER35S
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLBC0020

   Plasmid

   #172776

   Purpose

   Level 0 module containing NT1_OEP7

   Depositor

   Benjamin Field

   Article

   Velay et al Plant Methods. 2022 May 26;18(1):69. doi

   Insert

   CDS of chloroplast outer envelope membrane protein OEP7
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   no

   Promoter

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-MS2-HA-DmRoquin_AD

   Plasmid

   #148531

   Purpose

   Mammalian Expression of DmRoquin

   Depositor

   Elisa Izaurralde

   Article

   Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

   Insert

   DmRoquin (roq Fly)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Pb mD3S4-dN

   Plasmid

   #180252

   Purpose

   P. berghei D3 (502-617) construct with N516T, S533N and N539Q mutations to abolish N-glycosylation was used for crystallization.

   Depositor

   Timothy Springer

   Article

   Feng et al Elife. 2021 Dec 23;10. pii: 74707. doi:

   Insert

   D3 of P. berghei HAP2 with N-glycosylation sites mutated
   

   Use

   Tags

   6His

   Expression

   Mammalian

   Mutation

   N516T, S533N and N539Q

   Promoter

   Available since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pML104-natR412

   Plasmid

   #162042

   Purpose

   Expresses Cas9 and contains natR412 guide which targets the natR gene

   Depositor

   Jolanda Van Leeuwen

   Article

   Parts et al Mol Syst Biol. 2021 May;17(5):e10138. do

   Insert

   natR412 guide
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pML107-natR854

   Plasmid

   #162043

   Purpose

   Expresses Cas9 and contains natR854 guide which targets the natR gene

   Depositor

   Jolanda Van Leeuwen

   Article

   Parts et al Mol Syst Biol. 2021 May;17(5):e10138. do

   Insert

   natR854 guide
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMAL-ccDockerin

   Plasmid

   #72200

   Purpose

   MBP-ccDockerin expression plasmid

   Depositor

   Timothy Whitehead

   Article

   Kowalsky et al Proteins. 2016 Dec;84(12):1914-1928. doi

   Insert

   Clostridium Cellulolyticum Type I Dockerin
   

   Use

   Tags

   His-Tag and Maltose Binding Protein

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDD2711

   Plasmid

   #175083

   Purpose

   Mammalian expression of Flag-tagged KSHV ORF45 carrying E223A-S226A/RC mutations

   Depositor

   Dirk Dittmer

   Article

   Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

   Insert

   KSHV ORF45 (ORF45 Kaposi's sarcoma-associated herpesvirus 8)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E223A, S226A, RC mutations

   Promoter

   Available since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDD2712

   Plasmid

   #175084

   Purpose

   Mammalian expression of Flag tagged KSHV ORF45 carrying E223A-S226A/RN2 mutations

   Depositor

   Dirk Dittmer

   Article

   Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

   Insert

   KSHV ORF45 (ORF45 Kaposi's sarcoma-associated herpesvirus 8)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E223A, S226A, RN2 mutations

   Promoter

   Available since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHAGE-eGFP-TAX1BP1 Y635A

    Plasmid

    #175772

    Purpose

    Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 Y635A

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    TAX1BP1 (TAX1BP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Y635A

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-eGFP-TAX1BP1 N637A

    Plasmid

    #175773

    Purpose

    Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 N637A

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    TAX1BP1 (TAX1BP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    N637A

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJH775_pJH_LldR_PlldR-sfGFP.2opal

    Plasmid

    #163734

    Purpose

    Lactate-inducible expression of green fluorescent protein encoding two opal codons

    Depositor

    Matthew Bennett

    Article

    Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

    Insert

    lldr gfp.2TGA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    gfp with first two leucine codons mutated to amber

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWSK129-stm2585-gp130

    Plasmid

    #174404

    Purpose

    Bacterial expression of sarA-gp130 chimera under native promoter.

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    sarA-IL6ST chimera
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Endogenous

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. UBC1cer40GEM

    Plasmid

    #172699

    Purpose

    Ubiquitous expression of 60meric GEM monomer

    Depositor

    Krishna Niyogi

    Article

    Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

    Insert

    monomer of 60mer GEM
    

    Use

    Unspecified

    Tags

    GFP

    Expression

    Plant

    Mutation

    Promoter

    UBC1

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. LentiCRISPRv2bleo-Nectin-2

    Plasmid

    #170295

    Purpose

    A knock-out vector for the mouse Nectin-2

    Depositor

    Michiyuki Matsuda

    Article

    Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

    Insert

    A gRNA targeting the mouse Nectin-2 gene.
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LentiCRISPRv2bleo-Necl-5

    Plasmid

    #170294

    Purpose

    A knock-out vector for the mouse Necl-5

    Depositor

    Michiyuki Matsuda

    Article

    Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

    Insert

    A gRNA targeting the mouse Necl-5 gene.
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJCC_084 Cas12i1 D647A

    Plasmid

    #171671

    Purpose

    For bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    Cas12i1 D647A
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed aspartate 647 to alanine

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMRBad-C-DiB-RM

    Plasmid

    #168478

    Purpose

    C-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168476: pET11a-N-DiB-RM to obtain DiB-RM‐split protein

    Depositor

    Jens Meiler

    Article

    Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

    Insert

    C-fragment of DiB-RM
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET11a-N-DiB-RM

    Plasmid

    #168476

    Purpose

    N-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168478: pMRBad-C-DiB-RM to obtain DiB-RM‐split protein

    Depositor

    Jens Meiler

    Article

    Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100

    Insert

    N-fragment of DiB-RM
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Dcp2C Δ5H-H1

    Plasmid

    #163584

    Purpose

    express Dcp2 FL-H1 at endogenous level in S.cerevisiae

    Depositor

    Michael Rosen

    Article

    Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

    Insert

    Dcp2 (DCP2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Truncation of aa1-326; the first HLM (EDQLKSYAEEQ…

    Promoter

    DCP2 promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Nup54 no FG-mEGFP494(2)

    Plasmid

    #164271

    Purpose

    Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of mEGFP

    Depositor

    Sanford Simon

    Article

    Pulupa et al Elife. 2020 Dec 21;9. pii: 60654. doi: 1

    Insert

    Nup54 (NUP54 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    deleted the FG region of Nup54

    Promoter

    CMV

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Nup54 no FG-mEGFP494(1)

    Plasmid

    #164270

    Purpose

    Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 6 amino acids at the carboxyl end of mEGFP

    Depositor

    Sanford Simon

    Article

    Pulupa et al Elife. 2020 Dec 21;9. pii: 60654. doi: 1

    Insert

    Nup54 (NUP54 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    deleted the FG region of Nup54

    Promoter

    CMV

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pNTI763 p(Z)-mCherry + UBC9 sgRNA

    Plasmid

    #164918

    Purpose

    CEN/ARS with P(Z)-mCherry controlled by the ZEM transcription factor, also encode UBC9 sgRNA

    Depositor

    Nicholas Ingolia

    Article

    Muller et al Science. 2020 Dec 11;370(6522). pii: 370

    Insert

    P(Z)-mcherry
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    P(Z), synthetic promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. SPIC_pet28a

    Plasmid

    #131654

    Purpose

    Bacterial expression of ETS gene SPIC

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    SPIC (SPIC Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGST-POLA1-N0

    Plasmid

    #160983

    Purpose

    Express a fragment of mouse Pola1 protein

    Depositor

    Zhiguo Zhang

    Article

    Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

    Insert

    POLA1 (Pola1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGST-POLA1-N1-2A

    Plasmid

    #160986

    Purpose

    Express a fragment of mouse Pola1 protein

    Depositor

    Zhiguo Zhang

    Article

    Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

    Insert

    POLA1 (Pola1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    W84A/D88A

    Promoter

    Available since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGST-POLA1-N2-2A

    Plasmid

    #160987

    Purpose

    Express a fragment of mouse Pola1 protein

    Depositor

    Zhiguo Zhang

    Article

    Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

    Insert

    POLA1 (Pola1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    W84A/D88A

    Promoter

    Available since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGST-POLA1-N2

    Plasmid

    #160985

    Purpose

    Express a fragment of mouse Pola1 protein

    Depositor

    Zhiguo Zhang

    Article

    Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

    Insert

    POLA1 (Pola1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGST-POLA1-N1

    Plasmid

    #160984

    Purpose

    Express a fragment of mouse Pola1 protein

    Depositor

    Zhiguo Zhang

    Article

    Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

    Insert

    POLA1 (Pola1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBSV2H_Psyn-sgRNAmreB

    Plasmid

    #149567

    Purpose

    mreB gRNA expression in B. burgdorferi

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    sgRNAmreB
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Psyn

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDOC-K-glmS-GFPrev

    Plasmid

    #158060

    Purpose

    Donor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the reverse orientation relative to the kanamycin resistance selectable marker

    Depositor

    Mark Webber

    Article

    Holden et al Microbiology (Reading). 2020 Nov 23. doi

    Insert

    Green Fluorescent Protein optimised for excitation with UV light
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    acpP

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28a-Avi-Coh (E154C)-HIS

    Plasmid

    #153447

    Purpose

    E. coli expression of Rc. CohE construct with E154C mutation.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.CohE
    

    Use

    Tags

    6xHis and Avi-tag

    Expression

    Bacterial

    Mutation

    E154C mutation on CohE

    Promoter

    T7 promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCRII-TOPO CMV-cGFP-SV40 pA + Laccase2 Exon 2

    Plasmid

    #91802

    Purpose

    Expresses the Laccase2 circular RNA when the upstream SV40 poly(A) signal fails to be used

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Methods. 2021 Apr 18. pii: S1046-2023(21

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCZGY921

    Plasmid

    #135092

    Purpose

    Transcriptional GFP reporter of acr-2 (cholinergic) expressing neurons in C. elegans

    Depositor

    Yishi Jin

    Article

    Jospin et al PLoS Biol. 2009 Dec;7(12):e1000265. doi:

    Insert

    Pacr-2
    

    Use

    Tags

    GFP and Synthetic Intron

    Expression

    Worm

    Mutation

    Promoter

    Pacr-2

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCAG-T3-HypaCAS9-pA

    Plasmid

    #131467

    Purpose

    Expresses HypaCas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii

    Article

    Ikeda et al Commun Biol. 2019 Oct 10;2:371. doi: 10.

    Insert

    Codon optimized HypaCas9
    

    Use

    CRISPR

    Tags

    Flag, NLS, and polyA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. S1_AscI_V5-gateway_Bsu36I

    Plasmid

    #128467

    Purpose

    destination vector with N-terminal V5 tag

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    destination vector with N-terminal V5 tag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. S2_AscI_HA-gateway_Bsu36I

    Plasmid

    #128468

    Purpose

    destination vector with N-terminal HA tag

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    destination vector with N-terminal HA tag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTrcHis-7D12Asp73TAG

    Plasmid

    #125266

    Purpose

    Expresses 7D12 anti-EGFR nanobodies with UAG codon at Asp73 for synthetic amino acid in combination with pEVOL-pAzF

    Depositor

    Angus Johnston

    Article

    Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

    Insert

    7D12 Asp73TAG
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PKAcs-nolinker-GFP

    Plasmid

    #108579

    Purpose

    Expresses PKAcs-GFP fusion in mammalian cells

    Depositor

    Robert Hughes

    Article

    Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

    Insert

    protein kinase A, catalytic subunit
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    H87Q and W196R

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PKAcs-G12X-GFP

    Plasmid

    #108588

    Purpose

    Expresses PKAcs-GFP fusion in mammalian cells with flexible linker

    Depositor

    Robert Hughes

    Article

    Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

    Insert

    protein kinase A, catalytic subunit
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    H87Q and W196R

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge