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Addgene

We narrowed to 9,352 results for: UTY

Showing: 8051 - 8100 of 9352 results
  1. pGBT9-GGA2-GAE

    Plasmid
    #199175
    Purpose
    Expression of GAL4 DNA-binding domain (BD)-GGA2-GAE domain fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Insert
    GGA2-GAE domain (GGA2 Human)
    Use
    Tags
    GAL4-DNA binding domain fragment
    Expression
    Yeast
    Mutation
    Contains Lys 545 Gln substitution
    Promoter
    ADH1
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. pSCL.178

    Plasmid
    #184999
    Purpose
    Express -Eco1 FANCF editing ncRNA and gRNA
    Depositor
    Insert
    Eco1: FANCF targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Use
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    FANCF donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pSCL.176

    Plasmid
    #184997
    Purpose
    Express -Eco1 RNF2 editing ncRNA and gRNA
    Depositor
    Insert
    Eco1: RNF2 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Use
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    RNF2 donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pSCL.179

    Plasmid
    #185000
    Purpose
    Express -Eco1 HEK4 editing ncRNA and gRNA
    Depositor
    Insert
    Eco1: HEK4 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Use
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    HEK4 donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. pTS2333_Tier3(Lenti_inverse)-YPet_Puro

    Plasmid
    #169664
    Purpose
    Tier-3 vector for stable integration via lentiviral transduction in inverted direction with polyA carrying a constitutive expression cassette for puromyine and Ypet (PCMV-5'LTR-Psi-gag-env-PRPBSA-YPet-p2A-PuroR-pA::A2-pA::A3-pA-WPRE-3'LTR)
    Depositor
    Insert
    PRPBSA-driven YPet and PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PhCMV / RPBSA
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pDD2604

    Plasmid
    #175088
    Purpose
    Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T604A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).
    Depositor
    Insert
    KSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S644A
    Promoter
    Available since
    Feb. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pDD2602

    Plasmid
    #175086
    Purpose
    Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T424A, T438A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).
    Depositor
    Insert
    KSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    T424A, T438A
    Promoter
    Available since
    Feb. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pDD2603

    Plasmid
    #175087
    Purpose
    Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T504A, T508A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).
    Depositor
    Insert
    KSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    T504A, T508A
    Promoter
    Available since
    Feb. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. pN1mcherry-NKD2EFMUT

    Plasmid
    #174333
    Purpose
    encodes human NKD2 132DFD134>AFA mutant (two D mutated to A)cDNA with a C-terminal mCherry fusion.
    Depositor
    Insert
    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    Use
    Tags
    mCherry tag
    Expression
    Mammalian
    Mutation
    NKD2132DFD134>AFA (substitutions of D132 and D…
    Promoter
    CMV
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. pBlastR-5xQUAS-EBFP2-GMRWhite

    Plasmid
    #165921
    Purpose
    Blasticidin resistant 5xQUAS EBFP2 GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening
    Depositor
    Insert
    Selectable QUAS Response Vector
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. pBlastR-5xQUAS-sfGFP-MiniWhite

    Plasmid
    #165922
    Purpose
    Blasticidin resistant 5xQUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening
    Depositor
    Insert
    Selectable QUAS Response Vector
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
  12. pBlastR-5xQUAS-EBFP2-MiniWhite

    Plasmid
    #165924
    Purpose
    Blasticidin resistant 5xQUAS EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening
    Depositor
    Insert
    Selectable QUAS Response Vector
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
  13. pBlastR-5xUAS-EBFP2-MiniWhite

    Plasmid
    #165912
    Purpose
    Blasticidin resistant 5xUAS EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening
    Depositor
    Insert
    Selectable UAS Response Vector
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
  14. RRRAA-5-mut-pHG165c

    Plasmid
    #170113
    Purpose
    Constitutively expressed, mutationally-enhanced chimeric transcription activator comprising the AraC DNA binding domain and the RhaR rhamnose ligand binding domains.
    Depositor
    Insert
    RRRAA-5-MUT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    5A14L, W116Q, E178K, S184T, S237L
    Promoter
    Available since
    Aug. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
  15. pBlastR-5xUAS-mCherry-GMRWhite

    Plasmid
    #165908
    Purpose
    Blasticidin resistant 5xUAS mCherry GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening
    Depositor
    Insert
    Selectable UAS Response Vector
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 30, 2021
    Availability
    Academic Institutions and Nonprofits only
  16. pBlastR-5xQUAS-mCherry-GMRWhite

    Plasmid
    #165920
    Purpose
    Blasticidin resistant 5xQUAS mCherry GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening
    Depositor
    Insert
    Selectable QUAS Response Vector
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 30, 2021
    Availability
    Academic Institutions and Nonprofits only
  17. pBlastR-12xLexAOp-sfGFP-MiniWhite

    Plasmid
    #165916
    Purpose
    Blasticidin resistant 12xLexAOp sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening
    Depositor
    Insert
    Selectable LexAOp Response Vector
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
  18. pBlastR-5xQUAS-mCherry-MiniWhite

    Plasmid
    #165923
    Purpose
    Blasticidin resistant 5xQUAS mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening
    Depositor
    Insert
    Selectable QUAS Response Vector
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 8051 - 8100 of 9352 results