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GW1-Peredox-mCherry

Plasmid

#32380

Depositor

Gary Yellen

Article

Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

Insert

Peredox-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pCS2-Jmjd3-F

Plasmid

#17440

Purpose

Expression of Jmjd3 in mammalian cells

Depositor

Kai Ge

Article

Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

Insert

JMJD3 (Kdm6b Mouse)

Tags

6xMyc and Flag

Expression

Mammalian

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pSIN4-CMV-K2M

Plasmid

#21164

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Klf4, cMyc (MYC, KLF4 Human)

Use

Lentiviral; Reprogramming

Expression

Mammalian

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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pSin Capsid

Plasmid

#127693

Purpose

Plasmid contains the Sindbis capsid gene and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.

Depositor

Justin English, Bryan Roth

Article

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

Sindbis Capsid

Use

Synthetic Biology

Available Since

July 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1 Flag Runx1 FL (P#1790)

Plasmid

#14585

Depositor

Matija Peterlin

Article

Jiang et al Mol Cell Biol. 2005 Dec . 25(24):10675-8

Insert

Runx1 (Runx1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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hIRE1a wt

Plasmid

#20744

Depositor

Fumihiko Urano

Article

Lipson et al PLoS ONE. 2008 . 3(2):e1648.

Insert

IRE1 (ERN1 Human)

Use

Gateway Cloning and Lentiviral

Expression

Mammalian

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

Plasmid

#50942

Purpose

Bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.

Depositor

Tobias Bonhoeffer, Mark Huebener, Tobias Rose

Article

Rose et al Science. 2016 Jun 10;352(6291):1319-22.

Has Service

AAV1

Insert

mRuby2-P2A-GCaMP6s

Use

AAV

Promoter

hSyn1

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shRNA beta-catenin

Plasmid

#18803

Purpose

3rd gen lentiviral vector for knocking down beta-catenin gene expression

Depositor

Bob Weinberg

Article

Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

Insert

b-catenin shRNA (CTNNB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

July 22, 2008

Availability

Academic Institutions and Nonprofits only

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HA-VHL wt-pBabe-puro

Plasmid

#19234

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

wt

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Drp1K38A

Plasmid

#45161

Depositor

Alexander van der Bliek, Richard Youle

Article

Smirnova et al J Cell Biol. 1998 Oct 19;143(2):351-8.

Insert

Drp1 (DNM1L Human)

Tags

HA (AYPYDVPDY)

Expression

Mammalian

Mutation

K38A

Promoter

CMV

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-SADB19N

Plasmid

#32630

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Insert

B19N

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA Arf6 DN T27N

Plasmid

#10831

Depositor

Thomas Roberts

Article

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf6 T27N (ARF6 Human)

Tags

HA

Expression

Mammalian

Mutation

T27N. Defective in GTP binding. Dominant negati…

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9)

Plasmid

#101178

Purpose

Human expression plasmid for SpCas9 Cluster 1 variant (HypaCas9): CMV-T7-hSpCas9-Cluster1(N692A, M694A, Q695A, H698A)-NLS(SV40)-3xFLAG

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

hSpCas9-Cluster1(N692A/M694A/Q695A/H698A)

Tags

3x FLAG and NLS (SV40)

Expression

Mammalian

Mutation

N692A/M694A/Q695A/H698A

Promoter

CMV

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-SypHluorin 4x (S4x)

Plasmid

#37005

Depositor

Stephen Heinemann, Yongling Zhu

Article

Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

Insert

SypHluorin 4x (Syp Rat)

Tags

HA

Expression

Mammalian

Mutation

Four copies of pHluorin inserted at site 184-185

Promoter

CMV

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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EMTB-3XGFP

Plasmid

#26741

Depositor

William Bement

Article

Miller et al Nat Cell Biol. 2009 Jan . 11(1):71-7.

Insert

ensconsin (MAP7 Human)

Use

Probe for microtubules

Tags

3x EGFP and Myc

Expression

Mammalian

Mutation

Only contains the microtubule binding-domain of e…

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pSLIK-Venus

Plasmid

#25734

Purpose

Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Venus fluorescent protein coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pYPet-His

Plasmid

#14031

Depositor

Patrick Daugherty

Article

Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

Insert

YPet

Tags

6xHis

Expression

Bacterial

Available Since

Feb. 14, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX-GTB

Plasmid

#26197

Depositor

Edward Callaway

Article

Haubensak et al Nature. 2010 Nov 11. 468(7321):270-6.

Insert

Enhanced green fluorescent protein, TVA, rabies B19 glycoprotein

Use

AAV and Cre/Lox; Adeno-associated virus

Mutation

TVA, rabies glycoprotein are mammalian codon opti…

Available Since

Nov. 11, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA-GFP-STAT1

Plasmid

#11987

Depositor

Steven Johnson

Insert

Signal transducer and activator of transcription 1 (STAT1 Human)

Tags

GFP

Expression

Mammalian

Mutation

A188T, Q718R

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

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VP16-ER alpha

Plasmid

#11351

Depositor

Donald McDonnell

Article

Chang et al Mol Cell Biol. 1999 Dec . 19(12):8226-39

Insert

ER alpha (ESR1 Human)

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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