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pAAVS1-P-CAG-DEST

Plasmid

#80490

Purpose

Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

Depositor

Knut Woltjen

Publication

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Type

Empty backbone

Use

Donor vector (human)

Expression

Mammalian

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pTriEx-mCherry-LOV2

Plasmid

#81041

Purpose

Mammalian or insect expression for wt LOV2

Depositor

Klaus Hahn

Publication

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LOV2

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV, p10

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLenti-FoxO1-Clover

Plasmid

#67759

Purpose

Fluorescent fusion protein for tracking Akt signaling

Depositor

Peter Rotwein

Publication

Gross et al J Cell Sci. 2015 Jun 3. pii: jcs.168773.

Insert

Fox01 (Foxo1 Mouse)

Use

Lentiviral

Tags

Clover

Mutation

Changed Serine 209 to Alanine, changed Histidine …

Promoter

EF1a

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus

Plasmid

#61629

Purpose

R2D2: Expresses a fusion of auxin-sensitive DII to nuclear 3xVenus and auxin-insensitive mDII to nuclear tdTomato, both from the Arabidopsis RPS5A promoter

Depositor

Dolf Weijers

Publication

Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:

Inserts

mDII
ntdTomato
DII
n3xVenus

Expression

Plant

Promoter

RPS5A

Available since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCAG_smFP FLAG

Plasmid

#59756

Purpose

encodes multiple copies of an epitope tag FLAG

Depositor

Loren Looger

Publication

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

smFP _FLAG

Expression

Mammalian

Promoter

CAG

Available since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pDCC6

Plasmid

#59985

Purpose

Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassette

Depositor

Peter Duchek

Publication

Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

Inserts

Cas9
U6-2>sgRNA

Use

CRISPR

Tags

3xFLAG

Promoter

U6-96Ab and hsp70Bb

Available since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato

Plasmid

#51082

Purpose

Cre dependent expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore

Depositor

Jonathan Ting

Has service

AAV1

Insert

GCaMP6s

Use

AAV

Tags

P2A-nls-dTomato

Expression

Mammalian

Promoter

hEF1a

Available since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

1 Data Hub Report

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GFP-ATG8(416)/GFP-AUT7(416)

Plasmid

#49425

Purpose

Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.

Depositor

Daniel Klionsky

Publication

Guan et al Mol Biol Cell. 2001 Dec;12(12):3821-38.

Insert

autophagy-related 8 (ATG8 Budding Yeast)

Tags

GFP

Expression

Bacterial and Yeast

Promoter

ATG8

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pRPR1_gRNA_handle_RPR1t

Plasmid

#49014

Purpose

gRNA expression empty vector (yeast)

Depositor

Timothy Lu

Publication

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

pRPR1

Available since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pDEST/LIfeAct-mCherry-N1

Plasmid

#40908

Depositor

Robin Shaw

Publication

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LifeAct

Tags

mCherry

Expression

Mammalian

Mutation

No stop codon to create c-terminal fusion

Promoter

CMV

Available since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pMXs-IP GFP-NBR1

Plasmid

#38283

Depositor

Noboru Mizushima

Publication

Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

Insert

Next to BRCA1 gene 1 protein (NBR1 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA3.1 puro Nodamura B2

Plasmid

#17228

Depositor

Christopher Sullivan

Publication

Sullivan et al J Virol. 2005 Jun . 79(12):7371-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Nodamura B2 (NoVsRNA1gp2 Nodamura Virus)

Expression

Mammalian

Mutation

2 silent point mutations

Available since

June 22, 2011

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

6 Articles

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EGFP-Rubicon

Plasmid

#28022

Depositor

Qing Zhong

Publication

Sun et al J Biol Chem. 2011 Jan 7. 286(1):185-91.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rubicon (RUBCN Human)

Tags

3xFlag and EGFP

Expression

Mammalian

Available since

March 22, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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GFP-TNFalpha

Plasmid

#28089

Depositor

Jennifer Stow

Publication

Manderson et al J Cell Biol. 2007 Jul 2. 178(1):57-69.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TNFalpha (Tnf Mouse)

Tags

EGFP

Expression

Mammalian

Available since

March 22, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pDestTol2pA2_ubi:EGFP

Plasmid

#27323

Purpose

MultiSite Gateway assembly vector, to generate transgenic zebrafish with ubiquitous expression of EGFP

Depositor

Leonard Zon

Publication

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ubiquitin:EGFP reporter

Use

Tol2 transposon

Promoter

–3.5 kb zebrafish ubiquitin (ubi) promoter

Available since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCMV-myc-LC3 (Atg8)

Plasmid

#24919

Depositor

Toren Finkel

Publication

Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LC3 (MAP1LC3B Human)

Tags

Myc

Expression

Mammalian

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCHERRY3

Plasmid

#24659

Depositor

Tanya Parish

Publication

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

mCherry

Expression

Bacterial

Available since

June 9, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pcDNA-Ikkb-FLAG WT

Plasmid

#23298

Depositor

Warner Greene

Publication

Geleziunas et al Mol Cell Biol. 1998 Sep . 18(9):5157-65.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Ikkbeta (IKBKB Human)

Tags

Flag

Expression

Mammalian

Available since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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Dominant negative Hsp90 beta

Plasmid

#22480

Depositor

William Sessa

Publication

Miao et al Arterioscler Thromb Vasc Biol. 2008 Jan

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Dominant negative Hsp90 (HSP90AB1 Human)

Tags

HA

Expression

Mammalian

Available since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pENTR/pTER+ (430-1)

Plasmid

#17453

Purpose

Gateway entry vector for shRNA expression under H1/TO promoter

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and RNAi

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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