We narrowed to 15,793 results for: grna

1. pLentiGuide-Puro/CD73-gRNA4

   Plasmid

   #132588

   Purpose

   Lentiviral gRNA vector targeting human CD73

   Depositor

   Robert Sobol

   Article

   Wilk et al Sci Rep. 2020 Jan 20;10(1):651. doi: 10.

   Insert

   CD73 (NT5E Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLH-sgRNA1-boxB-MS2

   Plasmid

   #75396

   Purpose

   sgRNA1-boxB-MS2

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   sgRNA1-boxB-MS2
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. SauCas9 EMX1-nicking sgRNA

   Plasmid

   #169860

   Purpose

   SauCas9 nicking sgRNA for EMX1

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   SauCas9 EMX1-nicking sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 promoter

   Available Since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTBL437 AAVS1 EQR sgRNA

   Plasmid

   #126447

   Purpose

   To cut the human AAVS1 locus for integration.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human AAVS1 safe-harbor locus with NGAG PAM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-NQL005-SOX2-sgRNA

   Plasmid

   #175553

   Purpose

   For transient expression of spCas9-nuclease and a sgRNA targeting the mouse SOX2 locus.

   Depositor

   Elzo de Wit

   Article

   Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

   Insert

   spCas9-nuclease and sgRNA against mouse SOX2 STOP Codon
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. sgRNA scaffold-spacer-human U6

   Plasmid

   #214697

   Purpose

   provide "sgRNA scaffold-spacer-human U6" fragment

   Depositor

   Yin Shen

   Article

   Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

   Insert

   sgRNA scaffold-spacer-human U6
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX459-sgRNA4-CTCF-3p-UTR

   Plasmid

   #195106

   Purpose

   Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting downstream of the human CTCF 3'UTR. Puromycin selection (2A-fusion).

   Depositor

   Job Dekker

   Article

   Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

   Insert

   sgRNA against CTCF 3' UTR region
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX459-sgRNA3-CTCF-3p-UTR

   Plasmid

   #195105

   Purpose

   Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting downstream of the human CTCF 3'UTR. Puromycin selection (2A-fusion).

   Depositor

   Job Dekker

   Article

   Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

   Insert

   sgRNA against CTCF 3' UTR region
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX459-sgRNA2-CTCF-prom

   Plasmid

   #195104

   Purpose

   Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting upstream of the human CTCF promoter. Puromycin selection (2A-fusion).

   Depositor

   Job Dekker

   Article

   Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

   Insert

   sgRNA against CTCF promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX459-sgRNA1-CTCF-prom

    Plasmid

    #195103

    Purpose

    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting upstream of the human CTCF promoter. Puromycin selection (2A-fusion).

    Depositor

    Job Dekker

    Article

    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

    Insert

    sgRNA against CTCF promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX459-HypaCas9-mR2-ANLN_sgRNA

    Plasmid

    #183876

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ANLN sgRNA for N-terminal tagging of anillin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ANLN sgRNA spacer (ANLN Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. CDKN1A (p21) targeting gRNA

    Plasmid

    #215319

    Purpose

    Expresses gRNA targeting CDKN1A (p21) and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pM115: CasRx control presgRNA

    Plasmid

    #166867

    Purpose

    U6-driven expression of control (non-targeting) presgRNA compatible with CasRx.

    Depositor

    Guei-Sheung Liu

    Article

    Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

    Insert

    non-targeting presgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX459-sgRNA3-RPB1-N-term

    Plasmid

    #195112

    Purpose

    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).

    Depositor

    Job Dekker

    Article

    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

    Insert

    sgRNA against first exon of RPB1 (N-terminal)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX459-sgRNA1-RPB1-N-term

    Plasmid

    #195110

    Purpose

    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).

    Depositor

    Job Dekker

    Article

    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

    Insert

    sgRNA against first exon of RPB1 (N-terminal)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBbdCas9S(-10TC)_Psyn-sgRNA500

    Plasmid

    #149583

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, Psyn

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.1-puro U6 sgRNA Oct4A -12

    Plasmid

    #50921

    Purpose

    U6 driven sgRNA targeting OCT4 isoform A -12 bp from TSS

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    Oct4A -12 sgRNA (POU5F1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKLV2-U6gRNA5(SOX2_v32_7-4)-PGKpuroBFP-W

    Plasmid

    #211986

    Purpose

    Express gRNA against SOX2 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting SOX2 (SOX2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKLV2-U6gRNA5(SOX2_v32_7-5)-PGKpuroBFP-W

    Plasmid

    #211987

    Purpose

    Express gRNA against SOX2 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting SOX2 (SOX2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKLV2-U6gRNA5(Tsc2-g2)-PGKpuroBFP-W

    Plasmid

    #105040

    Purpose

    Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Tsc2 (Tsc2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTZ tRNA_gRNA empty scaffold

    Plasmid

    #188450

    Purpose

    Expression of gRNAs using a human tRNA promoter

    Depositor

    Ajit Chande

    Article

    Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tRNA glutamine

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pX601‐mhCMV‐miniABEmax‐N2‐ E53ogRNA

    Plasmid

    #187065

    Purpose

    Gp41-1 Split N-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation

    Depositor

    Renzhi Han

    Article

    Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

    Insert

    miniABEmax-Gp41-1
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mhCMV

    Available Since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pX601‐mhCMV‐ABEmaxNGA‐C2‐ E53ogRNA

    Plasmid

    #187066

    Purpose

    Gp41-1 Split C-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation

    Depositor

    Renzhi Han

    Article

    Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

    Insert

    Gp41-1-ABEmaxNGA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mhCMV

    Available Since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pU6-pegRNA-SNCA-A30P

    Plasmid

    #180016

    Purpose

    Transiently expressing a pegRNA to introduce SNCA-A30P mutation in human cells

    Depositor

    Dirk Hockemeyer

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    Prime editing pegRNA for SNCA-A30P (SNCA Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pYES2-gRNA-hyg-MCS

    Plasmid

    #107734

    Purpose

    E. coli-S. cerevisiae shuttle plasmid harbors gRNA expression cassettes targeting S. cerevisiae chromosomal X-3 site (HXK1 gene). Can function as a gRNA expression empty backbone plasmid.

    Depositor

    Sheng Yang

    Article

    Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

    Insert

    X-3 site gRNA expression cassettes
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52p

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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26. DmU6 3p3 GFP LgRNA

    Plasmid

    #182207

    Purpose

    For generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Drosophila

    Depositor

    Craig Montell

    Article

    Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

    Insert

    3XP3-GFP
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pX459-HypaCas9-mR2-ACTB_sgRNA

    Plasmid

    #183884

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ACTB sgRNA for N-terminal tagging of beta-actin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ACTB sgRNA spacer (ACTB Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pX459-HypaCas9-mR2-TUBA1B_sgRNA

    Plasmid

    #183888

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    TUBA1B sgRNA spacer (TUBA1B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pX459-HypaCas9-mR2-RHOA_sgRNA

    Plasmid

    #183877

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and RHOA sgRNA for N-terminal tagging of RhoA in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    RHOA sgRNA spacer (RHOA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pX458_miR-290-295KO-gRNA1

    Plasmid

    #172709

    Purpose

    Cas9 with 5' gRNA for paired CRISPR KO of miR-290-295 cluster

    Depositor

    Constance Ciaudo

    Article

    Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

    Insert

    miR-290-295
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge