163,606 results

1. pGfaABC1D-nLac

   Plasmid

   #53131

   Purpose

   expression of lacZ driven by human GfaABC1D promoter

   Depositor

   Michael Brenner

   Article

   Lee et al Glia. 2008 Apr;56(5):481-93. doi: 10.100

   Insert

   Gfa2 (GFAP Human)
   

   Use

   Mouse Targeting

   Tags

   LacZ, NLS, and mP1

   Expression

   Mammalian

   Mutation

   contains ABC1D regions of Gfa2 promoter, -1757 to…

   Promoter

   human Gfa2

   Available Since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. tetO.Nfib.Hygro

   Plasmid

   #117271

   Purpose

   Expresses Nfib and hygromycin resistance gene under control of tetO sequence.

   Depositor

   Henrik Ahlenius

   Article

   Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

   Insert

   Nfib (Nfib Mouse)
   

   Use

   Lentiviral

   Tags

   IRES and hygromycin resistant cassette (C termina…

   Expression

   Mammalian

   Mutation

   Promoter

   tet-on

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENN.AAV.hSyn.Cre.WPRE.hGH

   Plasmid

   #105553

   Purpose

   AAV expression of Cre from hSyn promoter

   Depositor

   James M. Wilson

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   Cre
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDF-Frb-v1

   Plasmid

   #201923

   Purpose

   E. coli vector expressing FrbA, FrbB, FrbC, FrbD and FrbE for the biosynthesis of non-hydrolyzable phosphoserine from phosphoenolpyruvate.

   Depositor

   Richard Cooley, Ryan Mehl

   Article

   Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

   Inserts

   FrbA
   FrbB
   FrbC
   FrbD
   FrbE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 (modified), T7 (modified, moderate), T7 (modif…

   Available Since

   June 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-sfGFP 150TAG

   Plasmid

   #85493

   Purpose

   pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

   Depositor

   Ryan Mehl

   Article

   Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

   Insert

   superfolder GFP 150TAG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   150 TAG (amino acid 150 mutated to stop codon)

   Promoter

   T7

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET30a-AlkB-D135S

   Plasmid

   #79051

   Purpose

   Express mutant AlkB protein (D135S) in E. coli BL21(DE3)

   Depositor

   Tao Pan

   Article

   Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

   Insert

   AlkB (alkB E.coli)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   A truncated AlkB with deletion of the amino (N)-t…

   Promoter

   T7

   Available Since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pERM2-nhpSer

   Plasmid

   #201922

   Purpose

   Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.

   Depositor

   Richard Cooley, Ryan Mehl

   Article

   Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

   Inserts

   SepRS(2)
   Sep-tRNA v2.0
   SerB
   EF-Sep
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   E412P, E414F, T417K, P495M, I496W, F529S and H67R…

   Promoter

   GlnS (constitutive), OXB20 (constitutive), lpp (c…

   Available Since

   June 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-TDP-43 (Q331K)

   Plasmid

   #235488

   Purpose

   Expression of human TDP-43-EGFP with human ALS-causing Q331K mutation

   Depositor

   Maxime Rousseaux

   Article

   Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

   Insert

   TARDBP (TARDBP Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Q331K

   Promoter

   Available Since

   April 30, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pSL3718 (pQCascade_Pse)

   Plasmid

   #200899

   Purpose

   Expresses human codon-optimized Pseudoalteromonas Cas7, Cas8, Cas6, and TniQ from a CMV promoter, in a polycistronic vector format separated by T2A sequences.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   PseCas7, PseCas8, PseCas6, PseTniQ
   

   Use

   Tags

   NLS (TniQ) and NLS, T2A (Cas7, Cas8, Cas6)

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFC333

    Plasmid

    #87844

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    ble (bleomycin resistance marker)
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-TetOne-Puro-p21

    Plasmid

    #171122

    Purpose

    Doxycycline inducible expression of p21

    Depositor

    Jason Sheltzer

    Article

    Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

    Insert

    p21 (CDKN1A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-PR rep/cap

    Plasmid

    #197565

    Purpose

    encodes AAV-PR capsid that transduces pericytes and smooth muscle cells in mice after systemic delivery

    Depositor

    Casey Maguire

    Article

    Ramirez et al Hum Gene Ther. 2023 Jun 27. doi: 10.1089

    Inserts

    AAV Rep genes
    AAV9 VP1 Cap gene with PR insert
    

    Use

    AAV

    Tags

    Expression

    Mutation

    contains 7mer insert PRPPSTH between amino acids …

    Promoter

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-Dcp1a

    Plasmid

    #153972

    Purpose

    Fluorescent protein fused to mRNA decapping enzyme 1a

    Depositor

    Gia Voeltz

    Article

    Lee et al Science. 2020 Jan 31;367(6477). pii: 367

    Insert

    mRNA decapping enzyme 1a (DCP1A Human)
    

    Use

    Tags

    pAcGFP-C1

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFUGW-FerH-ffLuc2-eGFP

    Plasmid

    #71393

    Purpose

    Lentiviral vector of luciferase-eGFP fusion gene driven by FerH promoter

    Depositor

    Glenn Merlino

    Article

    Day et al Pigment Cell Melanoma Res. 2009 Jun;22(3

    Insert

    FerH-ffLuc2-eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    FerH

    Available Since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. enAsABE8e

    Plasmid

    #138506

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-dAsCas12a (En)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCherry-TOMM20-N-10

    Plasmid

    #55146

    Purpose

    Localization: Outer Mitochondrial Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    TOMM20 (TOMM20 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSFFV_sfCherry3C(1-10)

    Plasmid

    #117482

    Purpose

    Lentiviral vector for constitutive expression of sfCherry3C(1-10)

    Depositor

    Bo Huang

    Article

    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

    Insert

    sfCherry3C(1-10)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    5 substitutions (K45R, G52D, T106A, K182R, N194D)…

    Promoter

    SFFV

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28:GFP

    Plasmid

    #60733

    Purpose

    pET28 driving expression of GFP, biobrick part Bba_E0040

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid

    #138147

    Purpose

    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    CasRx
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. FL-fibulin-3-V5-lentiviral-GFP

    Plasmid

    #29703

    Depositor

    Mariano Viapiano

    Article

    Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

    Insert

    EFEMP1 (EFEMP1 Human)
    

    Use

    Lentiviral

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 23, 2011

    Availability

    Academic Institutions and Nonprofits only