We narrowed to 4,881 results for: phy

1. pHDE-35S-Cas9-mCherry-UBQ

   Plasmid

   #78932

   Purpose

   For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants. Enable production of two gRNAs

   Depositor

   Yunde Zhao

   Article

   Gao et al Plant Physiol. 2016 May 15. pii: pp.0066

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV[Exp]-Puro-CMV>{ARL13B_6gly_GFP_6Gly_R_GECO1}

   Plasmid

   #232880

   Purpose

   Expresses the ARL13B coupled to GFP and RGECO1 calcium sensor.

   Depositor

   Paul DeCaen

   Article

   DeCaen et al J Cell Physiol. 2025 Apr;240(4):e70034.

   Insert

   ADP-ribosylation factor-like protein 13B (ARL13B Human)
   

   Use

   Lentiviral

   Tags

   GFP and RGECO1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3-HA-HA-humanCMYC

   Plasmid

   #74164

   Purpose

   Mammalian expression of human cMyc

   Depositor

   Martine Roussel

   Article

   Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

   Insert

   cMyc (MYC Human)
   

   Use

   Tags

   2x HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWC2

   Plasmid

   #15960

   Depositor

   Jeff Gelles

   Article

   Subramanian et al J Gen Physiol. 2007 Nov . 130(5):445-55.

   Insert

   Kinesin 1-401 BCCP his tag (Khc Fly, E. coli)
   

   Use

   Tags

   BCCP and His6

   Expression

   Bacterial

   Mutation

   amino acids 1-401

   Promoter

   Available since

   Nov. 27, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-mOXT-hM3Dq-mCherry-WPRE

   Plasmid

   #70717

   Purpose

   Expresses DREADDs Gq receptor under oxytocin promoter control

   Depositor

   Daniel Geschwind

   Article

   Penagarikano et al Sci Transl Med. 2015 Jan 21;7(271):271ra

   Insert

   hM3Dq-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   mouse oxytocin

   Available since

   Jan. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET16b-PCNA

   Plasmid

   #134898

   Purpose

   Bacterial expression and purification of human PCNA

   Depositor

   Andrew Deans

   Article

   Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

   Insert

   PCNA (PCNA Human)
   

   Use

   Tags

   untagged

   Expression

   Bacterial

   Mutation

   fully synthetic, codon optimized for bacterial ex…

   Promoter

   T7

   Available since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-hPOMC1-26-sbGLuc-P2A-dTomato

   Plasmid

   #176704

   Purpose

   neural activity dependent release of sbGLuc from presynaptic vesicles

   Depositor

   Ute Hochgeschwender

   Article

   Prakash et al Commun Biol. 2022 Jan 11;5(1):33. doi: 1

   Inserts

   Gaussia luciferase, slow burn
   dTomato
   

   Use

   AAV

   Tags

   P2A and hPOMC1-26

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL4-ERSE1-luc2P-Hygro

   Plasmid

   #101789

   Purpose

   Luferase reporter plasmid containing three tandem repeats of the ER stress response element-I (ERSE-I)

   Depositor

   Seiichi Oyadomari

   Article

   Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208

   Insert

   3xERSE-I
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   minimal TATA-box promoter with low basal activity

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAF112

   Plasmid

   #135920

   Purpose

   All-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with luciferse and hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.

   Depositor

   Andrew S. Flies

   Article

   Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

   Type

   Empty backbone

   Use

   Luciferase; Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCI-neo:alpha4-eGFP

    Plasmid

    #45068

    Depositor

    Henry Lester

    Article

    Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

    Insert

    alpha4-eGFP (Chrna4 Mouse)
    

    Use

    Tags

    Green Fluorescent Protien and HA Tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. FR_HNF1A

    Plasmid

    #31104

    Depositor

    Gerhart Ryffel

    Article

    Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

    Insert

    HNF1A (HNF1A Human)
    

    Use

    Flp/frt

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRK5-myc-Miro2 T18N

    Plasmid

    #47897

    Purpose

    Expresses myc tagged Miro2 T18N dominant negative mutant

    Depositor

    Pontus Aspenström

    Article

    Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

    Insert

    Miro2 T18N (RHOT2 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    T18N, dominant negative mutant

    Promoter

    CMV

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5-myc-Miro2 A13V

    Plasmid

    #47896

    Purpose

    Expresses myc tagged Miro2 A13V constitutively active mutant

    Depositor

    Pontus Aspenström

    Article

    Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

    Insert

    Miro2 A13V (RHOT2 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    A13V, constitutively active mutant

    Promoter

    CMV

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

14. eSpCas9(1.1)-Puro

    Plasmid

    #117686

    Purpose

    The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.

    Depositor

    Luc Leyns

    Article

    Liu et al Biochem Biophys Res Commun. 2019 Feb 14.

    Insert

    eSpCas9(1.1)
    

    Use

    CRISPR

    Tags

    PuroR (R166H)

    Expression

    Mammalian

    Mutation

    K848A, K1003A, R1060A

    Promoter

    U6

    Available since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHLmMBP-8

    Plasmid

    #72347

    Purpose

    Mammalian expression of cytoplasmic mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

    Depositor

    Luca Jovine

    Article

    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

    Type

    Empty backbone

    Use

    Tags

    6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer + chicken beta-actin promoter

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBItetDT12nGFP

    Plasmid

    #80420

    Purpose

    tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 12 CTG repeats in exon 15 located

    Depositor

    Thomas Cooper

    Article

    Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

    Insert

    EGFP and human DMPK exons 11-15 with 12 CTG repeats (DMPK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDGB3alpha2_35S:P19:Tnos (GB1203)

    Plasmid

    #68214

    Purpose

    Transcriptional unit for Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    P19 silencing suppressor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA

    Plasmid

    #61594

    Purpose

    A catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.

    Depositor

    Feng Zhang

    Article

    Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

    Insert

    hSaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    D10A, N580A

    Promoter

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CaMKII-EGFPKASH

    Plasmid

    #124882

    Purpose

    labelling of neuronal nuclear envelope with EGFP

    Depositor

    Vassilis Pachnis

    Article

    Obata et al Nature. 2020 Feb;578(7794):284-289. doi:

    Insert

    EGFP
    

    Use

    AAV

    Tags

    KASH

    Expression

    Mutation

    Promoter

    CaMKII

    Available since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRS306-PHIS3-GFP-μNS

    Plasmid

    #116935

    Purpose

    Yeast integrative vector for the expression of μNS

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    μNS
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    HIS3

    Available since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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