-
-
pCDNA3-HA/Bmal L95E
Plasmid#47344PurposePcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003DepositorInsertBMAL (Bmal1 Mouse)
UseTagsHaExpressionMammalianMutationL95EPromoterCMVAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
Islr-AP-His
Plasmid#71950PurposeExpresses the entire Islr protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertIslr (Islr Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-MyosinIIA-N-14
Plasmid#58117PurposeLocalization: Cytoskeleton, Excitation: 554, Emission: 581DepositorInsertMyosinIIA
UseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
Anti-INPP4b [N171/17.4R]
Plasmid#114551PurposeMammalian Expression Plasmid of anti-INPP4b (Human). Derived from hybridoma N171/17.4.DepositorInsertanti-INPP4b (Homo sapiens) recombinant mouse monoclonal antibody (INPP4B Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
CAG-mCherry-CrxshRNA
Plasmid#73987PurposeCrx shRNA (mir30 backbone) was inserted into the 3'UTR of mCherry.DepositorInsertshRNA that targets the mouse Crx gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMiniT:myc-GluA1 TKIT donor
Plasmid#169419PurposeDonor DNA for TKIT knockin of Myc to GluA1 in mouseDepositorInsertMyc (Myc Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pAAV-CMV-dSa VP64 Neurog2
Plasmid#99695PurposeExpresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Neurog2 promoter, vector allows for activation of mouse Neurog2, can be packaged and delivered as AAVDepositorArticleInsertdCas9 and gRNA targeting Neurog2 (gRNA: GGTATATAAGGGGTTTTAAG) (Neurog2 Synthetic, Mouse)
UseAAV and CRISPRTagsVP64ExpressionMutationdead Cas9PromoterAvailable sinceSept. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-tdTomato-FlagAkt2W80A
Plasmid#64834PurposecDNA of Akt2 with point mutation from Trp to Ala at position 80. This mutation confers resistance to MK-2206 drug.DepositorInsertAkt2 (Akt2 Mouse)
UseLentiviralTags3xFlagExpressionMammalianMutationPoint mutation Trp 80 to AlaPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
RCAS(A) mPax1 (CT#1039)
Plasmid#13902DepositorInsertPax1 (Pax1 Mouse)
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
Slax mMyf5 (CT#1008)
Plasmid#13913DepositorInsertMyf5 (Myf5 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgSmad4#1/Cre
Plasmid#173617PurposeExpresses a Smad4-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Smad4 (Smad4 Mouse)
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-1 Sesn1b
Plasmid#61874Purposebacterial expression of a GST-Sesn1b (mouse)DepositorInsertSesn1 (Sesn1 Mouse)
UseTagsGSTExpressionBacterialMutationnonePromoterAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
Anti-nNOS/NOS1 [L121/25R]
Plasmid#177466PurposeMammalian Expression Plasmid of anti-nNOS/NOS1 (Human). Derived from hybridoma L121/25.DepositorInsertanti-nNOS/NOS1 (Homo sapiens) recombinant mouse monoclonal antibody (NOS1 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
Anti-MECP2 [N227/21R]
Plasmid#177518PurposeMammalian Expression Plasmid of anti-MECP2 (Human). Derived from hybridoma N227/21.DepositorInsertanti-MECP2 (Homo sapiens) recombinant mouse monoclonal antibody (MECP2 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pSIN-Arkadia-gRNA2
Plasmid#180362Purposetargeting mouse Arkadia geneDepositorInsertArkadia targeting gRNA (Rnf111 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 SIRT1-deltaExon2
Plasmid#105671PurposeMammalian expression construct of mouse SIRT1 short isoform (lacking Exon2)DepositorInsertSIRT1 (Sirt1 Mouse)
UseTagsExpressionMammalianMutationSynonymous base changes not affecting the protein…PromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Anti-NGL-3/LRRC4B [N51/6.2R]
Plasmid#114493PurposeMammalian Expression Plasmid of anti-NGL-3/LRRC4B (Rat). Derived from hybridoma N51/6.2.DepositorInsertanti-NGL-3/LRRC4B (Rattus norvegicus) recombinant mouse monoclonal antibody (Lrrc4b Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM-mouse TCR zeta/2451
Plasmid#11506DepositorInsertTCR zeta (Cd247 Mouse)
UseGeneral cloningTagsExpressionMutationn/aPromoterAvailable sinceMarch 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Sema4D-CD4-FLAG
Plasmid#51601Purposeexpresses non-cleavable Sema4D/CD4 chimera with extracellular Sema4D, intracellular CD4DepositorInsertSema4D/CD4-FLAG (Sema4d Mouse, Human)
UseTagsFLAG and mycExpressionMammalianMutationSema4D amino acids 660-861 were replaced with CD4…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-nAChr-beta3
Plasmid#48811PurposeThis report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.DepositorInsertnAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)
UseTagsExpressionMammalianMutation*PromoterCMVAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCI-MOR271-1
Plasmid#22376DepositorInsertMOR271-1 (Olfr168 Mouse)
UseTagsRhoExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEX-KG-MmUbc7/2783
Plasmid#11490DepositorInsertMmUbc7 (Ube2g2 Mouse)
UseTagsGSTExpressionBacterialMutationn/aPromoterAvailable sinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
mEos2-MAP4-C-10
Plasmid#57399PurposeLocalization: Microtubules, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertMAP4
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG-FLAG-IRES-blasticidin-Sox2 S248A
Plasmid#182047Purposeexpression of Sox2 mutantsDepositorInsertSox2 (Sox2 Mouse)
UseTagsFLAGExpressionMammalianMutationS248APromoterCAGAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
FAM19A1-V5
Plasmid#184337PurposeExpression of V5-tagged mouse FAM19A1DepositorInsertFAM19A1 (Tafa1 Mouse)
UseTagsV5-tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-IRES-blasticidin-Sox2 S248A/T258A
Plasmid#182049Purposeexpression of Sox2 mutantsDepositorInsertSox2 (Sox2 Mouse)
UseTagsFLAGExpressionMammalianMutationS248A/T258APromoterCAGAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-IRES-blasticidin-Sox2 T258A
Plasmid#182048Purposeexpression of Sox2 mutantsDepositorInsertSox2 (Sox2 Mouse)
UseTagsFLAGExpressionMammalianMutationT258APromoterCAGAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV_PIG_3XHA-Ago1
Plasmid#170916PurposeExpresses 3X-HA-AGO1DepositorInsertAgo1 (Ago1 Mouse)
UseTags3X-HAExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Px458-Cas9n-Trp63-Exon4-2sgRNA
Plasmid#88849PurposeCRISPR KO of Trp63DepositorInsertTrp63 (Trp63 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET32b-FLJ23445
Plasmid#15434DepositorInsertFLJ23445
UseTagsHis and TrxExpressionBacterialMutationN/APromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
DT050A
Plasmid#159411PurposeConstitutive murine mature BDNF expression, controlled under E.coli J23101 (strong) promoterDepositorInsertpr.J23101-BDNF (Bdnf, Mouse) (Bdnf Synthetic, Mouse)
UseSynthetic BiologyTagsFLAGExpressionBacterialMutationPromoterJ23101 promoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCWXPG-UBI-PDX1:VP16
Plasmid#114305PurposeConstitutive expression of a PDX1:VP16 fusion proteinDepositorInsertPDX1-VP16 (Pdx1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
HBM-mVEGF-C-HisTag-pENTR
Plasmid#64066PurposePlasmid encodes mature form of murine VEGF-C preceded by honey bee melittin secretion signal; entry vector to Gateway cloning system.DepositorInsertvascular endothelial growth factor C (Vegfc Mouse)
UseTagsHis-tagExpressionInsectMutationPromoterAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-TMEM216-N-18
Plasmid#54280PurposeLocalization: Cilium, Excitation: 487, Emission: 509DepositorInsertTMEM216 (Tmem216 Mouse)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mCardinal-MannII-N-10
Plasmid#56167PurposeLocalization: Mannosidase/Golgi, Excitation: 604, Emission: 659DepositorInsertMannII
UseTagsmCardinalExpressionMammalianMutationaa 1-112 of MannIIPromoterCMVAvailable sinceMarch 4, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mILK-L207W-EFGP
Plasmid#176900Purposemouse ILK with L207W point mutation cloned into pEGFP-N1DepositorInsertIntegrin-linked kinase (Ilk Mouse)
UseTagsEGFPExpressionMammalianMutationLeucine 207 to TryptophanePromoterCNVAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only
