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Plasmid#180627PurposeBacterial expression for SPASTIN MIT, residues 112-196. Has N-term HIS-SUMO tag. Used for X-ray crystallography. Internal ID:WISP21-111.DepositorInsertSPASTIN
UseTagsHIS-SUMOExpressionBacterialMutationResidues 112-196PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA2_ZIKV C
Plasmid#145359PurposeCell-free/mammalian expression vector for Zika virus capsid protein with N-term mCherryDepositorInsertZIKV Capsid
UseTags8xHis and mCherryExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TGFB1
Plasmid#67080PurposeStandard for cell-free expression benchmarking.DepositorInsertTGFB1 (TGFB1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO-TtAgocatdead
Plasmid#210322PurposeBacterial expression of the His- and SUMO-tagged catalytically inactive Argonaute protein of Thermus thermophilusDepositorInsertTtAgo (TT_P0026 Thermus thermophilus)
UseTags6x His tag and SUMO tagExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pCDF-NtXNtTcR1AtR2NtB2
Plasmid#160889PurposeExpression of N. tabacum Rubisco small subunit (rbcS-Tc), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-Tc
UseTagsExpressionBacterialMutationPromoterrbcS-Tc: T7, chaperones: T12Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-sapphireAKAR(T/A)
Plasmid#118465PurposeNegative-control mutant for sapphireAKAR biosensor.DepositorInsertsapphireAKAR(T/A)
UseTags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
K401-iLid
Plasmid#122484PurposeComponent of optically-induced motor dimerization systemDepositorInsertKinesin 1-401 iLid His tag
UseTagsHis6 and iLidExpressionBacterialMutationKinesin amino acids 1-401PromoterT7Available sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a - APX
Plasmid#89618PurposeExpresses recombinant 6HIS tagged T. cruzi ascorbate-dependent tryparedoxin peroxidase protein in E. coli upon IPTG inductionDepositorInsertAscorbate dependent Tryparedoxin peroxidase
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBSFI-myr-akt-S473A
Plasmid#49190PurposecDNA of Akt1 with myristyolation signal and point mutation from S to A at position 473 in cloning vectorDepositorInsertAkt1 (Akt1 Mouse)
UseAdaptor plasmidTagsHA tag and myristolation signalExpressionMutationS473APromoterT7Available sinceDec. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
His-TEV-GB1 Leu5Met
Plasmid#128686PurposeExpresses GB1 Leu5Met mutantDepositorInsertspg
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pT7-th-sgRNA
Plasmid#65565Purposein vitro trancription of sgRNA targeting the zebrafish th locusDepositorInsertth sgRNA (th Zebrafish)
UseIn vitro rna transcriptionTagsExpressionMutationPromoterT7Available sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pmCellFree_KA2_ZIKV PrM
Plasmid#145360PurposeCell-free/mammalian expression vector for Zika virus PrM protein with N-term mCherryDepositorInsertZIKV PrM
UseTags8xHis and mCherryExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceMarch 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKCsub-YPet-NES
Plasmid#138207PurposeEncodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertPKCsub-YPet-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET14b_tev_PSGU
Plasmid#59738PurposeExpresses a.a. 417-887 of human ZO-1DepositorInsertzonula occludens-1 (TJP1 Human)
UseTagsHIS tag and thrombin clvgExpressionBacterialMutationPromoterT7Available sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
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NpT7-5-ERK1
Plasmid#39229DepositorInsertERK1 (MAPK3 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-HA2-GFP-HiBit
Plasmid#171017PurposeFor bacterial expression of bdSUMO tagged muGFP with HA2 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-HA2-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP6_rpoA-C
Plasmid#190966PurposeTest the C-to-U editing activity of KP6 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP6
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
IFI16-FL-(1-729)
Plasmid#35051DepositorInsertIFI16 (IFI16 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TFAM
Plasmid#67064PurposeStandard for cell-free expression benchmarking.DepositorInsertTFAM (TFAM Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationS115CPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
Affibody EGFR:1907.BirA*-pET301/NT-DEST
Plasmid#174691PurposePlasmid encoding for the bacterial expression of a bispecific coding for the anti-EGFR affibody ZEGFR:1907 fused to the mutated form of BirA enzyme, BirA*DepositorInsertAnti-EGFR affibody ZEGFR:1907 fused to mutated form of BirA, BirA*
UseTagsHIS tagExpressionBacterialMutationR118G mutation in BirAPromoterT7 promoterAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
6xHis-TEV-UBC-9
Plasmid#87055PurposeExpresses 6xHis-tagged ubc-9, with a TEV protease cleavage site between the tag and the ORFDepositorInsertUBC-9 (ubc-9 Nematode)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-baPrs-hdNadV
Plasmid#91950PurposepET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyiDepositorInsertsPutative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
Phosphoribosyl Pyrophosphate Synthetases
UseTagsExpressionBacterialMutationchanged Leucine 135 to IsoleucinePromoterT7Available sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - TR
Plasmid#89621Purpose[Edit] Expresses recombinant 6HIS tagged T. cruzi trypanothione reductase protein in E. coli upon IPTG inductionDepositorInsertThioredoxin
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtT2R1AtR2NtB2
Plasmid#160886PurposeExpression of N. tabacum Rubisco small subunit (rbcS-T2), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-T2
UseTagsExpressionBacterialMutationPromoterrbcS-T2: T7, chaperones: T9Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
TTR C10S S85C K80D
Plasmid#184743Purposebacterial expression of human TTR mutantsDepositorInsertTTR (TTR Human)
UseTagsExpressionBacterialMutationC10S, S85C, K80DPromoterT7Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM6-At4CL-CmCHS-MsCHI
Plasmid#73394PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsAt4CL
CmCHS
MsCHI
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-HIS-ddFLN4-K10
Plasmid#101718PurposeK10 peptide with ddFLN4 fingerprint domain and N-terminal ybbr tag for covalent immobilization - resulting in high forcesDepositorInsertddFLN4 fingerprint, K10 peptide (KRT10 Dictyostelium discoideum, Human)
UseTagsHIS and ybbrExpressionBacterialMutationC18S in ddFLN4PromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK_ToeRepG2_N02_trigger
Plasmid#132745PurposeT7 RNAP-driven expression of second-generation toehold repressor index 2 trigger RNADepositorInsertToeRepG2_N02_trigger
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA2_ZIKV NS4B
Plasmid#145367PurposeCell-free/mammalian expression vector for Zika virus NS4Bprotein with N-term mCherryDepositorInsertZIKV NS4B
UseTags8xHis and mCherryExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBTK102
Plasmid#110574PurposeBTK Type 2 promoter for golden gate assemblyDepositorInsertT7 promoter with RBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
E. coli 16S toehold switch sensor
Plasmid#110698PurposeToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP outputDepositorInsertE. coli 16S toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-blueCKAR(T/A)
Plasmid#118473PurposeNegative-control mutant for blueCKAR biosensor.DepositorInsertblueCKAR(T/A)
UseTags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-rsGreen1
Plasmid#78180PurposeExpresses rsGreen1 in E. coli strains.DepositorInsertrsGreen1
UseTags6xHis-tag; Xpress tagExpressionBacterialMutationPromoterT7Available sinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
Foxn1-pBSII SK
Plasmid#41032DepositorInsertFoxn1 in situ probe (Foxn1 Mouse)
UseIn situ probeTagsExpressionMutationPromoterT7 sense; T3 antisenseAvailable sinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pETM6-At4CL-CmCHS-CmCHI
Plasmid#73395PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsAt4CL
CmCHS
CmCHI
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
B. fragilis 16S toehold switch sensor
Plasmid#110696PurposeToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP outputDepositorInsertB. fragilis 16S toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only